Preoperative evaluation of microencapsulated human parathyroid tissue aids selection of the optimal bioartificial graft for human parathyroid allotransplantation
Summary Allotransplantation of microencapsulated parathyroid tissue is a promising approach to the treatment of permanent hypoparathyroidism. Preoperative assessment of the quality of microencapsulated parathyroid tissue could facilitate selection of the optimal bioartifical graft for human parathyr...
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| Veröffentlicht in: | Transplant international 2007-08, Vol.20 (8), p.688-696 |
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| creator | Moskalenko, Vasiliy Ulrichs, Karin Kerscher, Andreas Blind, Eberhard Otto, Christoph Hamelmann, Wulf Demidchik, Yurij Timm, Stephan |
| description | Summary
Allotransplantation of microencapsulated parathyroid tissue is a promising approach to the treatment of permanent hypoparathyroidism. Preoperative assessment of the quality of microencapsulated parathyroid tissue could facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation. Parathyroid tissue from patients with secondary hyperparathyroidism (n = 15) was processed mechanically or enzymatically (collagenase type II). Tissue particles and single cells/cell clusters were routinely microencapsulated with amitogenic Ba2+ alginate. Parathyroid secretion dynamics in response to stimulation of nonencapsulated and microencapsulated parathyroid tissue with Ca2+ were evaluated in a perifusion system. The stability of the different types of microcapsule was assessed using an osmotic pressure test. Mechanical cutting of parathyroid tissue led to peripheral necrosis of tissue particles and impaired their vitality. Collagenase digestion, in contrast, resulted in single cells and cell clusters without peripheral necrosis. The quality of microencapsulation of single cells/cell clusters was significantly better than that of tissue particles (deformed and imperfect capsules). Microencapsulation itself did not decrease cell vitality. Nonencapsulated and microencapsulated tissue particles and single cells/cell clusters from different donors maintained their own levels of response to stimulation with low Ca2+. Microcapsules containing tissue particles showed poor stability compared with those containing single cells/cell clusters. Preoperative evaluation of microencapsulated parathyroid tissue can disclose differences in vitality and function and thus facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation. |
| doi_str_mv | 10.1111/j.1432-2277.2007.00496.x |
| format | Article |
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Allotransplantation of microencapsulated parathyroid tissue is a promising approach to the treatment of permanent hypoparathyroidism. Preoperative assessment of the quality of microencapsulated parathyroid tissue could facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation. Parathyroid tissue from patients with secondary hyperparathyroidism (n = 15) was processed mechanically or enzymatically (collagenase type II). Tissue particles and single cells/cell clusters were routinely microencapsulated with amitogenic Ba2+ alginate. Parathyroid secretion dynamics in response to stimulation of nonencapsulated and microencapsulated parathyroid tissue with Ca2+ were evaluated in a perifusion system. The stability of the different types of microcapsule was assessed using an osmotic pressure test. Mechanical cutting of parathyroid tissue led to peripheral necrosis of tissue particles and impaired their vitality. Collagenase digestion, in contrast, resulted in single cells and cell clusters without peripheral necrosis. The quality of microencapsulation of single cells/cell clusters was significantly better than that of tissue particles (deformed and imperfect capsules). Microencapsulation itself did not decrease cell vitality. Nonencapsulated and microencapsulated tissue particles and single cells/cell clusters from different donors maintained their own levels of response to stimulation with low Ca2+. Microcapsules containing tissue particles showed poor stability compared with those containing single cells/cell clusters. Preoperative evaluation of microencapsulated parathyroid tissue can disclose differences in vitality and function and thus facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation.</description><identifier>ISSN: 0934-0874</identifier><identifier>EISSN: 1432-2277</identifier><identifier>DOI: 10.1111/j.1432-2277.2007.00496.x</identifier><identifier>PMID: 17511831</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alginates - pharmacology ; Biocompatible Materials - pharmacology ; Capsules ; Cell Transplantation - methods ; Cells, Cultured ; Drug Carriers ; Follow-Up Studies ; Glucuronic Acid - pharmacology ; Hexuronic Acids - pharmacology ; Humans ; Hypoparathyroidism - pathology ; Hypoparathyroidism - surgery ; in vitro function ; Matrix Metalloproteinase 8 - pharmacology ; microencapsulation ; parathyroid allotransplantation ; Parathyroid Glands - cytology ; Parathyroid Glands - metabolism ; Parathyroid Glands - transplantation ; Parathyroid Hormone - secretion ; parathyroid insufficiency ; Preoperative Care - methods ; Tissue and Organ Procurement - methods ; Transplantation, Homologous ; Treatment Outcome</subject><ispartof>Transplant international, 2007-08, Vol.20 (8), p.688-696</ispartof><rights>Copyright Blackwell Publishing Ltd. Aug 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4746-4b18efad88637c8b06152cc413a4efab3d3faa4fdbb8b6374fb8df16f27d19b43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1432-2277.2007.00496.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1432-2277.2007.00496.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17511831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moskalenko, Vasiliy</creatorcontrib><creatorcontrib>Ulrichs, Karin</creatorcontrib><creatorcontrib>Kerscher, Andreas</creatorcontrib><creatorcontrib>Blind, Eberhard</creatorcontrib><creatorcontrib>Otto, Christoph</creatorcontrib><creatorcontrib>Hamelmann, Wulf</creatorcontrib><creatorcontrib>Demidchik, Yurij</creatorcontrib><creatorcontrib>Timm, Stephan</creatorcontrib><title>Preoperative evaluation of microencapsulated human parathyroid tissue aids selection of the optimal bioartificial graft for human parathyroid allotransplantation</title><title>Transplant international</title><addtitle>Transpl Int</addtitle><description>Summary
Allotransplantation of microencapsulated parathyroid tissue is a promising approach to the treatment of permanent hypoparathyroidism. Preoperative assessment of the quality of microencapsulated parathyroid tissue could facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation. Parathyroid tissue from patients with secondary hyperparathyroidism (n = 15) was processed mechanically or enzymatically (collagenase type II). Tissue particles and single cells/cell clusters were routinely microencapsulated with amitogenic Ba2+ alginate. Parathyroid secretion dynamics in response to stimulation of nonencapsulated and microencapsulated parathyroid tissue with Ca2+ were evaluated in a perifusion system. The stability of the different types of microcapsule was assessed using an osmotic pressure test. Mechanical cutting of parathyroid tissue led to peripheral necrosis of tissue particles and impaired their vitality. Collagenase digestion, in contrast, resulted in single cells and cell clusters without peripheral necrosis. The quality of microencapsulation of single cells/cell clusters was significantly better than that of tissue particles (deformed and imperfect capsules). Microencapsulation itself did not decrease cell vitality. Nonencapsulated and microencapsulated tissue particles and single cells/cell clusters from different donors maintained their own levels of response to stimulation with low Ca2+. Microcapsules containing tissue particles showed poor stability compared with those containing single cells/cell clusters. Preoperative evaluation of microencapsulated parathyroid tissue can disclose differences in vitality and function and thus facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation.</description><subject>Alginates - pharmacology</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Capsules</subject><subject>Cell Transplantation - methods</subject><subject>Cells, Cultured</subject><subject>Drug Carriers</subject><subject>Follow-Up Studies</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Humans</subject><subject>Hypoparathyroidism - pathology</subject><subject>Hypoparathyroidism - surgery</subject><subject>in vitro function</subject><subject>Matrix Metalloproteinase 8 - pharmacology</subject><subject>microencapsulation</subject><subject>parathyroid allotransplantation</subject><subject>Parathyroid Glands - cytology</subject><subject>Parathyroid Glands - metabolism</subject><subject>Parathyroid Glands - transplantation</subject><subject>Parathyroid Hormone - secretion</subject><subject>parathyroid insufficiency</subject><subject>Preoperative Care - methods</subject><subject>Tissue and Organ Procurement - methods</subject><subject>Transplantation, Homologous</subject><subject>Treatment Outcome</subject><issn>0934-0874</issn><issn>1432-2277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFZDFgl2C_xI7EhtU8VOpEqgqa8tObMYjJw6203YehzfF6QwgVZWoN762v3N0fQ8AEKMal_VuV2NGSUUI5zVBiNcIsa6tb5-Azd-Hp2CDOsoqJDg7AS9S2iGEiGjQc3CCeYOxoHgDfn2LJswmquyuDTTXyi-lDBMMFo6uj8FMvZrT4lU2A9wuo5rgrAq-3cfgBphdSouByg0JJuNN_0ectwaGObtReahdUDE763pXTj-ishnaEB-wU96HHNWUZq-mfNfJS_DMKp_Mq-N-Cr5_-nh19qW6-Pr5_OzDRdUzztqKaSyMVYMQLeW90KjFDel7hqli5V7TgVqlmB20FrogzGoxWNxawgfcaUZPwduD7xzDz8WkLEeXeuNLIyYsSXLUiq4j_L8gQR1CbbOCb-6Bu7DEqXxCEtw1FDeoKZA4QGXWKUVj5RzL0OJeYiTXsOVOrpnKNVO5hi3vwpa3Rfr66L_o0Qz_hMd0C_D-ANw4b_aPNpZXl-eloL8BxI-9yQ</recordid><startdate>200708</startdate><enddate>200708</enddate><creator>Moskalenko, Vasiliy</creator><creator>Ulrichs, Karin</creator><creator>Kerscher, Andreas</creator><creator>Blind, Eberhard</creator><creator>Otto, Christoph</creator><creator>Hamelmann, Wulf</creator><creator>Demidchik, Yurij</creator><creator>Timm, Stephan</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7U9</scope><scope>7X8</scope></search><sort><creationdate>200708</creationdate><title>Preoperative evaluation of microencapsulated human parathyroid tissue aids selection of the optimal bioartificial graft for human parathyroid allotransplantation</title><author>Moskalenko, Vasiliy ; Ulrichs, Karin ; Kerscher, Andreas ; Blind, Eberhard ; Otto, Christoph ; Hamelmann, Wulf ; Demidchik, Yurij ; Timm, Stephan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4746-4b18efad88637c8b06152cc413a4efab3d3faa4fdbb8b6374fb8df16f27d19b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Alginates - pharmacology</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Capsules</topic><topic>Cell Transplantation - methods</topic><topic>Cells, Cultured</topic><topic>Drug Carriers</topic><topic>Follow-Up Studies</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Humans</topic><topic>Hypoparathyroidism - pathology</topic><topic>Hypoparathyroidism - surgery</topic><topic>in vitro function</topic><topic>Matrix Metalloproteinase 8 - pharmacology</topic><topic>microencapsulation</topic><topic>parathyroid allotransplantation</topic><topic>Parathyroid Glands - cytology</topic><topic>Parathyroid Glands - metabolism</topic><topic>Parathyroid Glands - transplantation</topic><topic>Parathyroid Hormone - secretion</topic><topic>parathyroid insufficiency</topic><topic>Preoperative Care - methods</topic><topic>Tissue and Organ Procurement - methods</topic><topic>Transplantation, Homologous</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moskalenko, Vasiliy</creatorcontrib><creatorcontrib>Ulrichs, Karin</creatorcontrib><creatorcontrib>Kerscher, Andreas</creatorcontrib><creatorcontrib>Blind, Eberhard</creatorcontrib><creatorcontrib>Otto, Christoph</creatorcontrib><creatorcontrib>Hamelmann, Wulf</creatorcontrib><creatorcontrib>Demidchik, Yurij</creatorcontrib><creatorcontrib>Timm, Stephan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplant international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moskalenko, Vasiliy</au><au>Ulrichs, Karin</au><au>Kerscher, Andreas</au><au>Blind, Eberhard</au><au>Otto, Christoph</au><au>Hamelmann, Wulf</au><au>Demidchik, Yurij</au><au>Timm, Stephan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preoperative evaluation of microencapsulated human parathyroid tissue aids selection of the optimal bioartificial graft for human parathyroid allotransplantation</atitle><jtitle>Transplant international</jtitle><addtitle>Transpl Int</addtitle><date>2007-08</date><risdate>2007</risdate><volume>20</volume><issue>8</issue><spage>688</spage><epage>696</epage><pages>688-696</pages><issn>0934-0874</issn><eissn>1432-2277</eissn><abstract>Summary
Allotransplantation of microencapsulated parathyroid tissue is a promising approach to the treatment of permanent hypoparathyroidism. Preoperative assessment of the quality of microencapsulated parathyroid tissue could facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation. Parathyroid tissue from patients with secondary hyperparathyroidism (n = 15) was processed mechanically or enzymatically (collagenase type II). Tissue particles and single cells/cell clusters were routinely microencapsulated with amitogenic Ba2+ alginate. Parathyroid secretion dynamics in response to stimulation of nonencapsulated and microencapsulated parathyroid tissue with Ca2+ were evaluated in a perifusion system. The stability of the different types of microcapsule was assessed using an osmotic pressure test. Mechanical cutting of parathyroid tissue led to peripheral necrosis of tissue particles and impaired their vitality. Collagenase digestion, in contrast, resulted in single cells and cell clusters without peripheral necrosis. The quality of microencapsulation of single cells/cell clusters was significantly better than that of tissue particles (deformed and imperfect capsules). Microencapsulation itself did not decrease cell vitality. Nonencapsulated and microencapsulated tissue particles and single cells/cell clusters from different donors maintained their own levels of response to stimulation with low Ca2+. Microcapsules containing tissue particles showed poor stability compared with those containing single cells/cell clusters. Preoperative evaluation of microencapsulated parathyroid tissue can disclose differences in vitality and function and thus facilitate selection of the optimal bioartifical graft for human parathyroid allotransplantation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17511831</pmid><doi>10.1111/j.1432-2277.2007.00496.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alginates - pharmacology Biocompatible Materials - pharmacology Capsules Cell Transplantation - methods Cells, Cultured Drug Carriers Follow-Up Studies Glucuronic Acid - pharmacology Hexuronic Acids - pharmacology Humans Hypoparathyroidism - pathology Hypoparathyroidism - surgery in vitro function Matrix Metalloproteinase 8 - pharmacology microencapsulation parathyroid allotransplantation Parathyroid Glands - cytology Parathyroid Glands - metabolism Parathyroid Glands - transplantation Parathyroid Hormone - secretion parathyroid insufficiency Preoperative Care - methods Tissue and Organ Procurement - methods Transplantation, Homologous Treatment Outcome |
| title | Preoperative evaluation of microencapsulated human parathyroid tissue aids selection of the optimal bioartificial graft for human parathyroid allotransplantation |
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