Cervical lymph cannulation to investigate the efflux and effects of intracerebroventricular cytokine infusions
It is well documented that there is communication between the cerebral spinal fluid (CSF) and cervical lymphatics. Recently, it has been demonstrated that tumor necrosis factor α (TNF-α) introduced into the CSF appears in the cervical lymph. However, the functional significance of this is less clear...
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| Veröffentlicht in: | Brain research. Brain research protocols 2001-02, Vol.6 (3), p.172-177 |
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| creator | Seabrook, Timothy J Dickstein, Jodi B Hay, John B |
| description | It is well documented that there is communication between the cerebral spinal fluid (CSF) and cervical lymphatics. Recently, it has been demonstrated that tumor necrosis factor α (TNF-α) introduced into the CSF appears in the cervical lymph. However, the functional significance of this is less clear. Here we describe a protocol to quantitate the efflux of TNF-α from the CSF into cervical lymph. In addition, we describe a methodology to examine the effects of an intracerebroventricular (i.c.v.) infusion of TNF-α on lymph volume, cellularity and cell phenotype. While TNF-α was recovered in the cervical lymph following infusion of
125-I labeled TNF-α, the dosage of TNF-α used in this study had no effect on cervical lymph flow, cellularity or cell subsets. This protocol can be used to study the efflux of i.c.v. injected macromolecules and their effects on lymphocytes in cervical lymph and the regional lymph nodes. |
| doi_str_mv | 10.1016/S1385-299X(00)00052-0 |
| format | Article |
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125-I labeled TNF-α, the dosage of TNF-α used in this study had no effect on cervical lymph flow, cellularity or cell subsets. This protocol can be used to study the efflux of i.c.v. injected macromolecules and their effects on lymphocytes in cervical lymph and the regional lymph nodes.</description><identifier>ISSN: 1385-299X</identifier><identifier>DOI: 10.1016/S1385-299X(00)00052-0</identifier><identifier>PMID: 11223417</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Catheterization - methods ; Cerebrospinal Fluid - metabolism ; Female ; Injections, Intraventricular - methods ; Intracerebroventricular injection ; Iodine Radioisotopes ; Lymph ; Lymph - metabolism ; Lymphatic ; Lymphocyte ; Neuroimmunomodulation - physiology ; Sheep ; Tumor necrosis factor α ; Tumor Necrosis Factor-alpha - pharmacokinetics</subject><ispartof>Brain research. Brain research protocols, 2001-02, Vol.6 (3), p.172-177</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c309t-b15045d78cb030f817469d8e410fa2b0394e1cb458196bb08b53f6f8561eb43c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11223417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seabrook, Timothy J</creatorcontrib><creatorcontrib>Dickstein, Jodi B</creatorcontrib><creatorcontrib>Hay, John B</creatorcontrib><title>Cervical lymph cannulation to investigate the efflux and effects of intracerebroventricular cytokine infusions</title><title>Brain research. Brain research protocols</title><addtitle>Brain Res Brain Res Protoc</addtitle><description>It is well documented that there is communication between the cerebral spinal fluid (CSF) and cervical lymphatics. Recently, it has been demonstrated that tumor necrosis factor α (TNF-α) introduced into the CSF appears in the cervical lymph. However, the functional significance of this is less clear. Here we describe a protocol to quantitate the efflux of TNF-α from the CSF into cervical lymph. In addition, we describe a methodology to examine the effects of an intracerebroventricular (i.c.v.) infusion of TNF-α on lymph volume, cellularity and cell phenotype. While TNF-α was recovered in the cervical lymph following infusion of
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| ispartof | Brain research. Brain research protocols, 2001-02, Vol.6 (3), p.172-177 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Catheterization - methods Cerebrospinal Fluid - metabolism Female Injections, Intraventricular - methods Intracerebroventricular injection Iodine Radioisotopes Lymph Lymph - metabolism Lymphatic Lymphocyte Neuroimmunomodulation - physiology Sheep Tumor necrosis factor α Tumor Necrosis Factor-alpha - pharmacokinetics |
| title | Cervical lymph cannulation to investigate the efflux and effects of intracerebroventricular cytokine infusions |
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