Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography
The objective of this research was to develop and validate analytical methods for quantitative determination of fluoroquinolones of third generation. Simple and rapid chromatographic method was developed and validated for quantitative determination of four quinolone antibiotics in tablets and inject...
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| creator | Santoro, Maria Inês R.M. Kassab, Nájla M. Singh, Anil K. Kedor-Hackmam, Erika R.M. |
| description | The objective of this research was to develop and validate analytical methods for quantitative determination of fluoroquinolones of third generation. Simple and rapid chromatographic method was developed and validated for quantitative determination of four quinolone antibiotics in tablets and injection preparations. The fluoroquinolones studied were gatifloxacin (GAT), levofloxacin (LEV), lomefloxacin (LOM) and pefloxacin (PEF). The quinolones were analyzed by using a LiChrospher
® 100 RP-18 column (5
μm, 125
mm
×
4
mm) and a mobile phase constituted of water:acetonitrile (80:20, v/v) with 0.3% of triethylamine and pH adjusted to 3.3 with phosphoric acid. The flow rate was 1.0
mL/min and the analyses were performed using UV detector with wavelengths varying from 279 to 295
nm. The analyses were performed at room temperature (24
±
2
°C). All fluoroquinolones were separated within 5
min. The calibration curves were linear (
r
≥
0.9999) over a concentration range from 4.0 to 24.0
μg/mL. The relative standard deviation (R.S.D.) was |
| doi_str_mv | 10.1016/j.jpba.2005.06.018 |
| format | Article |
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® 100 RP-18 column (5
μm, 125
mm
×
4
mm) and a mobile phase constituted of water:acetonitrile (80:20, v/v) with 0.3% of triethylamine and pH adjusted to 3.3 with phosphoric acid. The flow rate was 1.0
mL/min and the analyses were performed using UV detector with wavelengths varying from 279 to 295
nm. The analyses were performed at room temperature (24
±
2
°C). All fluoroquinolones were separated within 5
min. The calibration curves were linear (
r
≥
0.9999) over a concentration range from 4.0 to 24.0
μg/mL. The relative standard deviation (R.S.D.) was <1.0% and average recovery was above 99.54%.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2005.06.018</identifier><identifier>PMID: 16095864</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Anti-Bacterial Agents - analysis ; Biological and medical sciences ; Calibration ; Chemistry Techniques, Analytical - methods ; Chemistry, Pharmaceutical - methods ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Ethylamines - analysis ; Fluoroquinolones ; Fluoroquinolones - analysis ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC ; Hydrogen-Ion Concentration ; Levofloxacin ; Medical sciences ; Models, Chemical ; Models, Statistical ; Ofloxacin - analysis ; Pefloxacin - analysis ; Pharmaceutical preparations ; Pharmaceutical Preparations - analysis ; Pharmacology. Drug treatments ; Quinolones - analysis ; Regression Analysis ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrophotometry, Ultraviolet - methods ; Tablets ; Temperature ; Time Factors</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2006-01, Vol.40 (1), p.179-184</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-2699cd2b94418959d450b60ed68dbce61e448a23b8d0fc49b92108f68bfd7e9b3</citedby><cites>FETCH-LOGICAL-c450t-2699cd2b94418959d450b60ed68dbce61e448a23b8d0fc49b92108f68bfd7e9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2005.06.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17508283$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16095864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santoro, Maria Inês R.M.</creatorcontrib><creatorcontrib>Kassab, Nájla M.</creatorcontrib><creatorcontrib>Singh, Anil K.</creatorcontrib><creatorcontrib>Kedor-Hackmam, Erika R.M.</creatorcontrib><title>Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>The objective of this research was to develop and validate analytical methods for quantitative determination of fluoroquinolones of third generation. Simple and rapid chromatographic method was developed and validated for quantitative determination of four quinolone antibiotics in tablets and injection preparations. The fluoroquinolones studied were gatifloxacin (GAT), levofloxacin (LEV), lomefloxacin (LOM) and pefloxacin (PEF). The quinolones were analyzed by using a LiChrospher
® 100 RP-18 column (5
μm, 125
mm
×
4
mm) and a mobile phase constituted of water:acetonitrile (80:20, v/v) with 0.3% of triethylamine and pH adjusted to 3.3 with phosphoric acid. The flow rate was 1.0
mL/min and the analyses were performed using UV detector with wavelengths varying from 279 to 295
nm. The analyses were performed at room temperature (24
±
2
°C). All fluoroquinolones were separated within 5
min. The calibration curves were linear (
r
≥
0.9999) over a concentration range from 4.0 to 24.0
μg/mL. The relative standard deviation (R.S.D.) was <1.0% and average recovery was above 99.54%.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Ethylamines - analysis</subject><subject>Fluoroquinolones</subject><subject>Fluoroquinolones - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Hydrogen-Ion Concentration</subject><subject>Levofloxacin</subject><subject>Medical sciences</subject><subject>Models, Chemical</subject><subject>Models, Statistical</subject><subject>Ofloxacin - analysis</subject><subject>Pefloxacin - analysis</subject><subject>Pharmaceutical preparations</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmacology. Drug treatments</subject><subject>Quinolones - analysis</subject><subject>Regression Analysis</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrophotometry, Ultraviolet - methods</subject><subject>Tablets</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMoTs_oC7iQbHQ1VSZ1SSXgZhi8wYAICu5CLifdaaoqZVLV2M_my5myW9qVq-Q_-XJO8v8IvaCkpISyN_tyP2lVVoS0JWElofwR2lDe1UXFmu-P0YZ0NS06wtsrdJ3SnmSQiuYpuqKMiJazZoN-fVnUOPtZzf4A2MIMcfBjVmHEweFt3rk-_FTGj7e4h0P4R4UB_iqsRouni3T9EmL4sfgx9GH0Bq9DtA-zNwnn82mn4qAMLLmgejxFmFT8MzVhfcQ7v90VE0QXMjUawL3PvSw2uxgGNYdtVNPu-Aw9capP8Py83qBv7999vf9YPHz-8On-7qEwTUvmbIYQxlZaNA3lohU2VzUjYBm32gCj0DRcVbXmljjTCC0qSrhjXDvbgdD1DXp96jutX4I0y8EnA32vRghLkh1hnLacZ7A6gSaGlCI4OUU_qHiUlMg1MrmXa2RyjUwSJnNk-dLLc_dFD2AvV84ZZeDVGVApm-VidsSnC9e1hFe8ztzbEwfZi4OHKJPxkN2zPoKZpQ3-f-_4DWErvG4</recordid><startdate>20060123</startdate><enddate>20060123</enddate><creator>Santoro, Maria Inês R.M.</creator><creator>Kassab, Nájla M.</creator><creator>Singh, Anil K.</creator><creator>Kedor-Hackmam, Erika R.M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060123</creationdate><title>Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography</title><author>Santoro, Maria Inês R.M. ; Kassab, Nájla M. ; Singh, Anil K. ; Kedor-Hackmam, Erika R.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-2699cd2b94418959d450b60ed68dbce61e448a23b8d0fc49b92108f68bfd7e9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Anti-Bacterial Agents - analysis</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Ethylamines - analysis</topic><topic>Fluoroquinolones</topic><topic>Fluoroquinolones - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Hydrogen-Ion Concentration</topic><topic>Levofloxacin</topic><topic>Medical sciences</topic><topic>Models, Chemical</topic><topic>Models, Statistical</topic><topic>Ofloxacin - analysis</topic><topic>Pefloxacin - analysis</topic><topic>Pharmaceutical preparations</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Pharmacology. Drug treatments</topic><topic>Quinolones - analysis</topic><topic>Regression Analysis</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Spectrophotometry, Ultraviolet - methods</topic><topic>Tablets</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santoro, Maria Inês R.M.</creatorcontrib><creatorcontrib>Kassab, Nájla M.</creatorcontrib><creatorcontrib>Singh, Anil K.</creatorcontrib><creatorcontrib>Kedor-Hackmam, Erika R.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santoro, Maria Inês R.M.</au><au>Kassab, Nájla M.</au><au>Singh, Anil K.</au><au>Kedor-Hackmam, Erika R.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2006-01-23</date><risdate>2006</risdate><volume>40</volume><issue>1</issue><spage>179</spage><epage>184</epage><pages>179-184</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>The objective of this research was to develop and validate analytical methods for quantitative determination of fluoroquinolones of third generation. Simple and rapid chromatographic method was developed and validated for quantitative determination of four quinolone antibiotics in tablets and injection preparations. The fluoroquinolones studied were gatifloxacin (GAT), levofloxacin (LEV), lomefloxacin (LOM) and pefloxacin (PEF). The quinolones were analyzed by using a LiChrospher
® 100 RP-18 column (5
μm, 125
mm
×
4
mm) and a mobile phase constituted of water:acetonitrile (80:20, v/v) with 0.3% of triethylamine and pH adjusted to 3.3 with phosphoric acid. The flow rate was 1.0
mL/min and the analyses were performed using UV detector with wavelengths varying from 279 to 295
nm. The analyses were performed at room temperature (24
±
2
°C). All fluoroquinolones were separated within 5
min. The calibration curves were linear (
r
≥
0.9999) over a concentration range from 4.0 to 24.0
μg/mL. The relative standard deviation (R.S.D.) was <1.0% and average recovery was above 99.54%.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16095864</pmid><doi>10.1016/j.jpba.2005.06.018</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0731-7085 |
| ispartof | Journal of pharmaceutical and biomedical analysis, 2006-01, Vol.40 (1), p.179-184 |
| issn | 0731-7085 1873-264X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70681588 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis Analytical, structural and metabolic biochemistry Anti-Bacterial Agents - analysis Biological and medical sciences Calibration Chemistry Techniques, Analytical - methods Chemistry, Pharmaceutical - methods Chromatography Chromatography, High Pressure Liquid - methods Ethylamines - analysis Fluoroquinolones Fluoroquinolones - analysis Fundamental and applied biological sciences. Psychology General pharmacology HPLC Hydrogen-Ion Concentration Levofloxacin Medical sciences Models, Chemical Models, Statistical Ofloxacin - analysis Pefloxacin - analysis Pharmaceutical preparations Pharmaceutical Preparations - analysis Pharmacology. Drug treatments Quinolones - analysis Regression Analysis Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet - methods Tablets Temperature Time Factors |
| title | Quantitative determination of gatifloxacin, levofloxacin, lomefloxacin and pefloxacin fluoroquinolonic antibiotics in pharmaceutical preparations by high-performance liquid chromatography |
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