Lysobacter koreensis sp. nov., isolated from a ginseng field

Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, South Korea Correspondence Deok-Chun Yang dcyang{at}khu.ac.kr Strain Dae16 T , a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from the soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain Dae16 T belongs to the Gammaproteobacteria and had the highest degree of sequence similarity to Lysobacter gummosus ATCC 29489 T (97·1 %), Lysobacter antibioticus DSM 2044 T (96·6 %), Lysobacter enzymogenes DSM 2043 T (96·2 %), Lysobacter concretionis KCTC 12205 T (94·7 %) and Lysobacter brunescens ATCC 29482 T (93·7 %). Chemotaxonomic data revealed that strain Dae16 T possesses a quinone system with Q-8 as the predominant compound and C 15 : 0 iso, C 16 : 0 iso and C 17 : 1 iso 9 c as the predominant iso-branched fatty acids, all of which corroborated the assignment of the strain to the genus Lysobacter . Results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain Dae16 T represents a distinct species. Based on these data, it is proposed that Dae16 T (=KCTC 12204 T =NBRC 101156 T ) should be classified as the type strain of a novel Lysobacter species, Lysobacter koreensis sp. nov. Published online ahead of print on 4 November 2005 as DOI 10.1099/ijs.0.63955-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Dae16 T is AB166878 .