Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells
We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage colony-...
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| Veröffentlicht in: | Cell death and differentiation 2006-02, Vol.13 (2), p.250-259 |
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| creator | Montesoro, E Castelli, G Morsilli, O Nisini, R Stafsnes, M H Carè, A Peschle, C Chelucci, C |
| description | We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage colony-stimulating factor or IL-4+c-Kit-ligand+FLT-3 ligand switched the Mo precursors into dendritic cells (DCs). The switching capacity decreased only at the end of the culture, when most Mo cells matured to macrophages. Moreover, the Mo precursors were highly susceptible to transduction with lentiviral vectors: once switched to DCs, they maintained the transgene expression, as well as the phenotype and function of the DC lineage. Our results provide new insight into the potential role of the Mo lineage as a reservoir of DCs
in vivo
. Furthermore, the methodology for transduction of Mo precursors provides a tool to generate genetically modified, normally functioning DCs potentially useful for immunotherapy. |
| doi_str_mv | 10.1038/sj.cdd.4401748 |
| format | Article |
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in vivo
. Furthermore, the methodology for transduction of Mo precursors provides a tool to generate genetically modified, normally functioning DCs potentially useful for immunotherapy.</description><identifier>ISSN: 1350-9047</identifier><identifier>EISSN: 1476-5403</identifier><identifier>DOI: 10.1038/sj.cdd.4401748</identifier><identifier>PMID: 16110321</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Antigens ; Apoptosis ; Biochemistry ; Biomedical and Life Sciences ; Bone marrow ; Cell Biology ; Cell Cycle Analysis ; Cell death ; Cell growth ; Cell Lineage ; Cell Proliferation - drug effects ; Cells, Cultured ; Cytokines ; Cytokines - pharmacology ; Dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - physiology ; Gene Expression Regulation ; Gene Transfer Techniques ; Genetic Vectors ; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology ; Granulocytes ; Hematology ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - drug effects ; Humans ; Immunotherapy ; Interleukin-4 - pharmacology ; Lentivirus - genetics ; Leukocytes ; Life Sciences ; Ligands ; Membrane Proteins - pharmacology ; Monocytes - chemistry ; Monocytes - cytology ; Monocytes - drug effects ; Myelopoiesis - drug effects ; Oncology ; original-paper ; Phenotype ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - analysis ; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics ; Stem Cell Factor - pharmacology ; Stem Cells ; Transduction, Genetic ; Transgenes ; Tumor necrosis factor-TNF</subject><ispartof>Cell death and differentiation, 2006-02, Vol.13 (2), p.250-259</ispartof><rights>Springer Nature Limited 2006</rights><rights>Copyright Nature Publishing Group Feb 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-1afdff962c15e23d7af496193698c9d623b62272dfe65dd1cc8b810c68f0f7413</citedby><cites>FETCH-LOGICAL-c422t-1afdff962c15e23d7af496193698c9d623b62272dfe65dd1cc8b810c68f0f7413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16110321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Montesoro, E</creatorcontrib><creatorcontrib>Castelli, G</creatorcontrib><creatorcontrib>Morsilli, O</creatorcontrib><creatorcontrib>Nisini, R</creatorcontrib><creatorcontrib>Stafsnes, M H</creatorcontrib><creatorcontrib>Carè, A</creatorcontrib><creatorcontrib>Peschle, C</creatorcontrib><creatorcontrib>Chelucci, C</creatorcontrib><title>Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells</title><title>Cell death and differentiation</title><addtitle>Cell Death Differ</addtitle><addtitle>Cell Death Differ</addtitle><description>We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage colony-stimulating factor or IL-4+c-Kit-ligand+FLT-3 ligand switched the Mo precursors into dendritic cells (DCs). The switching capacity decreased only at the end of the culture, when most Mo cells matured to macrophages. Moreover, the Mo precursors were highly susceptible to transduction with lentiviral vectors: once switched to DCs, they maintained the transgene expression, as well as the phenotype and function of the DC lineage. Our results provide new insight into the potential role of the Mo lineage as a reservoir of DCs
in vivo
. Furthermore, the methodology for transduction of Mo precursors provides a tool to generate genetically modified, normally functioning DCs potentially useful for immunotherapy.</description><subject>Antigens</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Bone marrow</subject><subject>Cell Biology</subject><subject>Cell Cycle Analysis</subject><subject>Cell death</subject><subject>Cell growth</subject><subject>Cell Lineage</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Cytokines - pharmacology</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - physiology</subject><subject>Gene Expression Regulation</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Vectors</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</subject><subject>Granulocytes</subject><subject>Hematology</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Humans</subject><subject>Immunotherapy</subject><subject>Interleukin-4 - pharmacology</subject><subject>Lentivirus - genetics</subject><subject>Leukocytes</subject><subject>Life Sciences</subject><subject>Ligands</subject><subject>Membrane Proteins - pharmacology</subject><subject>Monocytes - chemistry</subject><subject>Monocytes - cytology</subject><subject>Monocytes - drug effects</subject><subject>Myelopoiesis - drug effects</subject><subject>Oncology</subject><subject>original-paper</subject><subject>Phenotype</subject><subject>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - analysis</subject><subject>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics</subject><subject>Stem Cell Factor - pharmacology</subject><subject>Stem Cells</subject><subject>Transduction, Genetic</subject><subject>Transgenes</subject><subject>Tumor necrosis factor-TNF</subject><issn>1350-9047</issn><issn>1476-5403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UUtvFSEUJsbG1urWnYa4cDe3wMzAjDvT-ErauLFrwoXDLVcGrsBo-o_8mTKZq02aNCE55JzvceBD6BUlG0ra4SLvN9qYTdcRKrrhCTqjneBN35H2ab23PWlG0olT9DznPSGEi5E_Q6eU08pm9Az9uQnOuwBqB3iKIeq7Eg_RQXYZu4BvYVJroziNDynuILgSE9azL3OC9zj_dkXfurDDC_VHlcIlgSoThIJVPd7j64gN_AIfD0tXeZxL9VsMzKxrNc5aSHXkVHEx1H5ZGMEkt9hq8D6_QCdW-Qwvj_Uc3Xz6-P3yS3P17fPXyw9Xje4YKw1V1lg7cqZpD6w1Qtlu5HRs-Tjo0XDWbjljghkLvDeGaj1sB0o0HyyxoqPtOXq36tbH_pwhFzm5vGygAsQ5S1H_kAtBKvDtA-A-zinU3SSjQrSc0L6CNitIp5hzAisPyU0q3UlK5BKgzHtZA5THACvhzVF13k5g7uHHxCrgYgXkOgo7SPe2j0q-XhlBLZH9l_w3_wsIpLYs</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Montesoro, E</creator><creator>Castelli, G</creator><creator>Morsilli, O</creator><creator>Nisini, R</creator><creator>Stafsnes, M H</creator><creator>Carè, A</creator><creator>Peschle, C</creator><creator>Chelucci, C</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060201</creationdate><title>Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells</title><author>Montesoro, E ; Castelli, G ; Morsilli, O ; Nisini, R ; Stafsnes, M H ; Carè, A ; Peschle, C ; Chelucci, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-1afdff962c15e23d7af496193698c9d623b62272dfe65dd1cc8b810c68f0f7413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Antigens</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Bone marrow</topic><topic>Cell Biology</topic><topic>Cell Cycle Analysis</topic><topic>Cell death</topic><topic>Cell growth</topic><topic>Cell Lineage</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>Cytokines - pharmacology</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - physiology</topic><topic>Gene Expression Regulation</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Vectors</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology</topic><topic>Granulocytes</topic><topic>Hematology</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Humans</topic><topic>Immunotherapy</topic><topic>Interleukin-4 - pharmacology</topic><topic>Lentivirus - genetics</topic><topic>Leukocytes</topic><topic>Life Sciences</topic><topic>Ligands</topic><topic>Membrane Proteins - pharmacology</topic><topic>Monocytes - chemistry</topic><topic>Monocytes - cytology</topic><topic>Monocytes - drug effects</topic><topic>Myelopoiesis - drug effects</topic><topic>Oncology</topic><topic>original-paper</topic><topic>Phenotype</topic><topic>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - analysis</topic><topic>Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics</topic><topic>Stem Cell Factor - pharmacology</topic><topic>Stem Cells</topic><topic>Transduction, Genetic</topic><topic>Transgenes</topic><topic>Tumor necrosis factor-TNF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Montesoro, E</creatorcontrib><creatorcontrib>Castelli, G</creatorcontrib><creatorcontrib>Morsilli, O</creatorcontrib><creatorcontrib>Nisini, R</creatorcontrib><creatorcontrib>Stafsnes, M H</creatorcontrib><creatorcontrib>Carè, A</creatorcontrib><creatorcontrib>Peschle, C</creatorcontrib><creatorcontrib>Chelucci, C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell death and differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Montesoro, E</au><au>Castelli, G</au><au>Morsilli, O</au><au>Nisini, R</au><au>Stafsnes, M H</au><au>Carè, A</au><au>Peschle, C</au><au>Chelucci, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells</atitle><jtitle>Cell death and differentiation</jtitle><stitle>Cell Death Differ</stitle><addtitle>Cell Death Differ</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>13</volume><issue>2</issue><spage>250</spage><epage>259</epage><pages>250-259</pages><issn>1350-9047</issn><eissn>1476-5403</eissn><abstract>We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage colony-stimulating factor or IL-4+c-Kit-ligand+FLT-3 ligand switched the Mo precursors into dendritic cells (DCs). The switching capacity decreased only at the end of the culture, when most Mo cells matured to macrophages. Moreover, the Mo precursors were highly susceptible to transduction with lentiviral vectors: once switched to DCs, they maintained the transgene expression, as well as the phenotype and function of the DC lineage. Our results provide new insight into the potential role of the Mo lineage as a reservoir of DCs
in vivo
. Furthermore, the methodology for transduction of Mo precursors provides a tool to generate genetically modified, normally functioning DCs potentially useful for immunotherapy.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>16110321</pmid><doi>10.1038/sj.cdd.4401748</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Apoptosis Biochemistry Biomedical and Life Sciences Bone marrow Cell Biology Cell Cycle Analysis Cell death Cell growth Cell Lineage Cell Proliferation - drug effects Cells, Cultured Cytokines Cytokines - pharmacology Dendritic cells Dendritic Cells - cytology Dendritic Cells - physiology Gene Expression Regulation Gene Transfer Techniques Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology Granulocytes Hematology Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - drug effects Humans Immunotherapy Interleukin-4 - pharmacology Lentivirus - genetics Leukocytes Life Sciences Ligands Membrane Proteins - pharmacology Monocytes - chemistry Monocytes - cytology Monocytes - drug effects Myelopoiesis - drug effects Oncology original-paper Phenotype Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - analysis Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - genetics Stem Cell Factor - pharmacology Stem Cells Transduction, Genetic Transgenes Tumor necrosis factor-TNF |
| title | Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells |
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