CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish ( Danio rerio)
We report a new cytochrome P450 (CYP) subfamily CYP3C and the cloning through PCR from zebrafish ( Danio rerio) of the first member, CYP3C1 ( AY499002). The CYP3C1 gene is on Chromosome 3 with 13 ORF exons encoding a 505 amino acid protein which has 44–54% identities with mammalian and teleost CYP3A...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-02, Vol.340 (4), p.1039-1046 |
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| creator | Corley-Smith, Graham E. Su, Hsiao-Ting Wang-Buhler, Jun-Lan Tseng, Hua-Pin Hu, Chin-Hwa Hoang, Thuy Chung, Woon-Gye Buhler, Donald R. |
| description | We report a new cytochrome P450 (CYP) subfamily CYP3C and the cloning through PCR from zebrafish (
Danio rerio) of the first member, CYP3C1 (
AY499002). The
CYP3C1 gene is on Chromosome 3 with 13 ORF exons encoding a 505 amino acid protein which has 44–54% identities with mammalian and teleost CYP3A and CYP3B forms. As evidenced by spectral analysis, the CYP3C1 protein heterologously expressed in yeast is functional. In silico analysis identified, on the same region of the chromosome, three more genes encoding CYP3C1-like proteins that formed a clade with CYP3C1 in a phylogenetic tree. Using RT-PCR, the CYP3C1 mRNA was detected in 1–6
dpf embryo/larvae and in adult fish liver and seven extrahepatic tissues. Whole-mount in situ hybridization using a riboprobe demonstrated expression in the brain during 12–120
hpf. At the 120
hpf larval stage, CYP3C1 mRNA was also detected in the pharynx and gastrointestinal tract. TCDD, dexamethasone, and rifampicin, which up-regulated CYP3A65 mRNA in zebrafish larvae, did not alter the CYP3C1 transcript levels suggesting regulatory differences between CYP3A and CYP3C enzymes in this species. |
| doi_str_mv | 10.1016/j.bbrc.2005.12.110 |
| format | Article |
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Danio rerio) of the first member, CYP3C1 (
AY499002). The
CYP3C1 gene is on Chromosome 3 with 13 ORF exons encoding a 505 amino acid protein which has 44–54% identities with mammalian and teleost CYP3A and CYP3B forms. As evidenced by spectral analysis, the CYP3C1 protein heterologously expressed in yeast is functional. In silico analysis identified, on the same region of the chromosome, three more genes encoding CYP3C1-like proteins that formed a clade with CYP3C1 in a phylogenetic tree. Using RT-PCR, the CYP3C1 mRNA was detected in 1–6
dpf embryo/larvae and in adult fish liver and seven extrahepatic tissues. Whole-mount in situ hybridization using a riboprobe demonstrated expression in the brain during 12–120
hpf. At the 120
hpf larval stage, CYP3C1 mRNA was also detected in the pharynx and gastrointestinal tract. TCDD, dexamethasone, and rifampicin, which up-regulated CYP3A65 mRNA in zebrafish larvae, did not alter the CYP3C1 transcript levels suggesting regulatory differences between CYP3A and CYP3C enzymes in this species.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.12.110</identifier><identifier>PMID: 16414346</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; AY499002 ; Base Sequence ; CYP3C1 ; Cytochrome P-450 Enzyme System - chemistry ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Cytochrome P450 ; Cytochromes - chemistry ; Cytochromes - genetics ; Cytochromes - metabolism ; Danio rerio ; Embryo ; Evolution, Molecular ; Molecular Sequence Data ; Organ Specificity ; Phylogenetic tree ; Phylogeny ; Sequence Homology, Amino Acid ; Teleostei ; Tissue Distribution ; Tissues ; Yeast ; Zebrafish ; Zebrafish - genetics ; Zebrafish - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2006-02, Vol.340 (4), p.1039-1046</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-3ecf95e5d112962f24c1be2a2c4c8d79935a5c1bacf6a1296fa9b7a79db116023</citedby><cites>FETCH-LOGICAL-c451t-3ecf95e5d112962f24c1be2a2c4c8d79935a5c1bacf6a1296fa9b7a79db116023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.12.110$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16414346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corley-Smith, Graham E.</creatorcontrib><creatorcontrib>Su, Hsiao-Ting</creatorcontrib><creatorcontrib>Wang-Buhler, Jun-Lan</creatorcontrib><creatorcontrib>Tseng, Hua-Pin</creatorcontrib><creatorcontrib>Hu, Chin-Hwa</creatorcontrib><creatorcontrib>Hoang, Thuy</creatorcontrib><creatorcontrib>Chung, Woon-Gye</creatorcontrib><creatorcontrib>Buhler, Donald R.</creatorcontrib><title>CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish ( Danio rerio)</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We report a new cytochrome P450 (CYP) subfamily CYP3C and the cloning through PCR from zebrafish (
Danio rerio) of the first member, CYP3C1 (
AY499002). The
CYP3C1 gene is on Chromosome 3 with 13 ORF exons encoding a 505 amino acid protein which has 44–54% identities with mammalian and teleost CYP3A and CYP3B forms. As evidenced by spectral analysis, the CYP3C1 protein heterologously expressed in yeast is functional. In silico analysis identified, on the same region of the chromosome, three more genes encoding CYP3C1-like proteins that formed a clade with CYP3C1 in a phylogenetic tree. Using RT-PCR, the CYP3C1 mRNA was detected in 1–6
dpf embryo/larvae and in adult fish liver and seven extrahepatic tissues. Whole-mount in situ hybridization using a riboprobe demonstrated expression in the brain during 12–120
hpf. At the 120
hpf larval stage, CYP3C1 mRNA was also detected in the pharynx and gastrointestinal tract. TCDD, dexamethasone, and rifampicin, which up-regulated CYP3A65 mRNA in zebrafish larvae, did not alter the CYP3C1 transcript levels suggesting regulatory differences between CYP3A and CYP3C enzymes in this species.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>AY499002</subject><subject>Base Sequence</subject><subject>CYP3C1</subject><subject>Cytochrome P-450 Enzyme System - chemistry</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochrome P450</subject><subject>Cytochromes - chemistry</subject><subject>Cytochromes - genetics</subject><subject>Cytochromes - metabolism</subject><subject>Danio rerio</subject><subject>Embryo</subject><subject>Evolution, Molecular</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Phylogenetic tree</subject><subject>Phylogeny</subject><subject>Sequence Homology, Amino Acid</subject><subject>Teleostei</subject><subject>Tissue Distribution</subject><subject>Tissues</subject><subject>Yeast</subject><subject>Zebrafish</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE-LFDEQR4O4uOPqF_AgOYmC3Val0-kJeJHxzwoL7kFBDxKSdIXJMN3ZTbpdxk_vjDPgzT0VFO_3Do-xZwg1Aqo3m9q57GsB0NYoakR4wBYIGiqBIB-yBQCoSmj8fs4el7IBQJRKP2LnqCTKRqoF-7n6cd2s8DWf1sRDzGXiAw2OMk-BWz7SHfe7Kfl1TgPxa9kCL7MLdojbHQ9pHnseR_6bXLYhljV_yd_bMSaeKcf06gk7C3Zb6OnpXrBvHz98XV1WV18-fV69u6q8bHGqGvJBt9T2iEIrEYT06EhY4aVf9p3WTWvb_cv6oOwBCVa7zna6d4gKRHPBXhy9NzndzlQmM8Tiabu1I6W5mA5UJ2W3vBcUsFSgEPagOII-p1IyBXOT42DzziCYQ32zMYf65lDfoDD4d_T8ZJ_dQP2_ySn3Hnh7BGgf41ekbIqPNHrqYyY_mT7F__n_AONDk7g</recordid><startdate>20060224</startdate><enddate>20060224</enddate><creator>Corley-Smith, Graham E.</creator><creator>Su, Hsiao-Ting</creator><creator>Wang-Buhler, Jun-Lan</creator><creator>Tseng, Hua-Pin</creator><creator>Hu, Chin-Hwa</creator><creator>Hoang, Thuy</creator><creator>Chung, Woon-Gye</creator><creator>Buhler, Donald R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060224</creationdate><title>CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish ( Danio rerio)</title><author>Corley-Smith, Graham E. ; Su, Hsiao-Ting ; Wang-Buhler, Jun-Lan ; Tseng, Hua-Pin ; Hu, Chin-Hwa ; Hoang, Thuy ; Chung, Woon-Gye ; Buhler, Donald R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-3ecf95e5d112962f24c1be2a2c4c8d79935a5c1bacf6a1296fa9b7a79db116023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>AY499002</topic><topic>Base Sequence</topic><topic>CYP3C1</topic><topic>Cytochrome P-450 Enzyme System - chemistry</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Cytochrome P450</topic><topic>Cytochromes - chemistry</topic><topic>Cytochromes - genetics</topic><topic>Cytochromes - metabolism</topic><topic>Danio rerio</topic><topic>Embryo</topic><topic>Evolution, Molecular</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Phylogenetic tree</topic><topic>Phylogeny</topic><topic>Sequence Homology, Amino Acid</topic><topic>Teleostei</topic><topic>Tissue Distribution</topic><topic>Tissues</topic><topic>Yeast</topic><topic>Zebrafish</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corley-Smith, Graham E.</creatorcontrib><creatorcontrib>Su, Hsiao-Ting</creatorcontrib><creatorcontrib>Wang-Buhler, Jun-Lan</creatorcontrib><creatorcontrib>Tseng, Hua-Pin</creatorcontrib><creatorcontrib>Hu, Chin-Hwa</creatorcontrib><creatorcontrib>Hoang, Thuy</creatorcontrib><creatorcontrib>Chung, Woon-Gye</creatorcontrib><creatorcontrib>Buhler, Donald R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corley-Smith, Graham E.</au><au>Su, Hsiao-Ting</au><au>Wang-Buhler, Jun-Lan</au><au>Tseng, Hua-Pin</au><au>Hu, Chin-Hwa</au><au>Hoang, Thuy</au><au>Chung, Woon-Gye</au><au>Buhler, Donald R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish ( Danio rerio)</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2006-02-24</date><risdate>2006</risdate><volume>340</volume><issue>4</issue><spage>1039</spage><epage>1046</epage><pages>1039-1046</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We report a new cytochrome P450 (CYP) subfamily CYP3C and the cloning through PCR from zebrafish (
Danio rerio) of the first member, CYP3C1 (
AY499002). The
CYP3C1 gene is on Chromosome 3 with 13 ORF exons encoding a 505 amino acid protein which has 44–54% identities with mammalian and teleost CYP3A and CYP3B forms. As evidenced by spectral analysis, the CYP3C1 protein heterologously expressed in yeast is functional. In silico analysis identified, on the same region of the chromosome, three more genes encoding CYP3C1-like proteins that formed a clade with CYP3C1 in a phylogenetic tree. Using RT-PCR, the CYP3C1 mRNA was detected in 1–6
dpf embryo/larvae and in adult fish liver and seven extrahepatic tissues. Whole-mount in situ hybridization using a riboprobe demonstrated expression in the brain during 12–120
hpf. At the 120
hpf larval stage, CYP3C1 mRNA was also detected in the pharynx and gastrointestinal tract. TCDD, dexamethasone, and rifampicin, which up-regulated CYP3A65 mRNA in zebrafish larvae, did not alter the CYP3C1 transcript levels suggesting regulatory differences between CYP3A and CYP3C enzymes in this species.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16414346</pmid><doi>10.1016/j.bbrc.2005.12.110</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2006-02, Vol.340 (4), p.1039-1046 |
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| subjects | Amino Acid Sequence Animals AY499002 Base Sequence CYP3C1 Cytochrome P-450 Enzyme System - chemistry Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Cytochrome P450 Cytochromes - chemistry Cytochromes - genetics Cytochromes - metabolism Danio rerio Embryo Evolution, Molecular Molecular Sequence Data Organ Specificity Phylogenetic tree Phylogeny Sequence Homology, Amino Acid Teleostei Tissue Distribution Tissues Yeast Zebrafish Zebrafish - genetics Zebrafish - metabolism |
| title | CYP3C1, the first member of a new cytochrome P450 subfamily found in zebrafish ( Danio rerio) |
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