Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni
To develop a method for assessing the relative epidemiological significance of possible infection sources for human campylobacteriosis. Using fluorescent amplified fragment length polymorphism (AFLP), 243 apparently epidemiologically unrelated Campylobacter jejuni isolates were genotyped (77 human,...
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| Veröffentlicht in: | Journal of applied microbiology 2006-02, Vol.100 (2), p.316-324 |
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| creator | Wieland, B Wittwer, M Regula, G Wassenaar, T.M Burnens, A.P Keller, J Stärk, K.D.C |
| description | To develop a method for assessing the relative epidemiological significance of possible infection sources for human campylobacteriosis. Using fluorescent amplified fragment length polymorphism (AFLP), 243 apparently epidemiologically unrelated Campylobacter jejuni isolates were genotyped (77 human, 46 cattle, 49 pet and 71 poultry isolates). In total 136 different phena were identified, of which 48 were clusters grouping at least two isolates. Isolates from different sources were frequently clustered together, underlining the high degree of source mixing and the lack of host specificity of C. jejuni. The phena were classified into different phenon types according to the sources of the isolates they contained. The occurrence of these phenon types was analysed using an area-proportional Euler diagram to describe epidemiological relatedness among C. jejuni isolates. Group separation statistics revealed that 43% of analysed human isolates expressed maximum similarity to other human isolates, 9% to cattle isolates, 21% to pet isolates and 27% to poultry isolates; these results were in accordance with the pattern observed in the phenon cluster analysis. Based on the grouping of strains into molecular similarity clusters, ecological patterns between sources can be investigated. This approach is a new methodological contribution to establish the relative epidemiological significance of concurrent infection sources. |
| doi_str_mv | 10.1111/j.1365-2672.2005.02788.x |
| format | Article |
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Using fluorescent amplified fragment length polymorphism (AFLP), 243 apparently epidemiologically unrelated Campylobacter jejuni isolates were genotyped (77 human, 46 cattle, 49 pet and 71 poultry isolates). In total 136 different phena were identified, of which 48 were clusters grouping at least two isolates. Isolates from different sources were frequently clustered together, underlining the high degree of source mixing and the lack of host specificity of C. jejuni. The phena were classified into different phenon types according to the sources of the isolates they contained. The occurrence of these phenon types was analysed using an area-proportional Euler diagram to describe epidemiological relatedness among C. jejuni isolates. Group separation statistics revealed that 43% of analysed human isolates expressed maximum similarity to other human isolates, 9% to cattle isolates, 21% to pet isolates and 27% to poultry isolates; these results were in accordance with the pattern observed in the phenon cluster analysis. Based on the grouping of strains into molecular similarity clusters, ecological patterns between sources can be investigated. This approach is a new methodological contribution to establish the relative epidemiological significance of concurrent infection sources.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2005.02788.x</identifier><identifier>PMID: 16430508</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>amplified fragment length polymorphism ; Animals ; Animals, Domestic - microbiology ; Biological and medical sciences ; Campylobacter Infections - epidemiology ; Campylobacter Infections - genetics ; Campylobacter Infections - transmission ; Campylobacter jejuni ; Campylobacter jejuni - isolation & purification ; campylobacteriosis ; Cat Diseases - epidemiology ; Cat Diseases - genetics ; Cat Diseases - transmission ; Cats ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - genetics ; Cattle Diseases - transmission ; Cluster Analysis ; Dog Diseases - epidemiology ; Dog Diseases - genetics ; Dog Diseases - transmission ; Dogs ; fluorescence ; Fundamental and applied biological sciences. Psychology ; Genotype ; host specificity ; Humans ; Microbiology ; mixed infection ; molecular epidemiology ; pets ; Polymorphism, Genetic - genetics ; Poultry ; Poultry Diseases - epidemiology ; Poultry Diseases - genetics ; Poultry Diseases - transmission ; source identification ; zoonoses ; Zoonoses - epidemiology ; Zoonoses - transmission</subject><ispartof>Journal of applied microbiology, 2006-02, Vol.100 (2), p.316-324</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Feb 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5298-9aa03b07bd11cec6cfae064f449f118d54d59c446b6ca8f760aa145c12661b3e3</citedby><cites>FETCH-LOGICAL-c5298-9aa03b07bd11cec6cfae064f449f118d54d59c446b6ca8f760aa145c12661b3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2005.02788.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2005.02788.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17577419$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16430508$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wieland, B</creatorcontrib><creatorcontrib>Wittwer, M</creatorcontrib><creatorcontrib>Regula, G</creatorcontrib><creatorcontrib>Wassenaar, T.M</creatorcontrib><creatorcontrib>Burnens, A.P</creatorcontrib><creatorcontrib>Keller, J</creatorcontrib><creatorcontrib>Stärk, K.D.C</creatorcontrib><title>Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>To develop a method for assessing the relative epidemiological significance of possible infection sources for human campylobacteriosis. Using fluorescent amplified fragment length polymorphism (AFLP), 243 apparently epidemiologically unrelated Campylobacter jejuni isolates were genotyped (77 human, 46 cattle, 49 pet and 71 poultry isolates). In total 136 different phena were identified, of which 48 were clusters grouping at least two isolates. Isolates from different sources were frequently clustered together, underlining the high degree of source mixing and the lack of host specificity of C. jejuni. The phena were classified into different phenon types according to the sources of the isolates they contained. The occurrence of these phenon types was analysed using an area-proportional Euler diagram to describe epidemiological relatedness among C. jejuni isolates. Group separation statistics revealed that 43% of analysed human isolates expressed maximum similarity to other human isolates, 9% to cattle isolates, 21% to pet isolates and 27% to poultry isolates; these results were in accordance with the pattern observed in the phenon cluster analysis. Based on the grouping of strains into molecular similarity clusters, ecological patterns between sources can be investigated. This approach is a new methodological contribution to establish the relative epidemiological significance of concurrent infection sources.</description><subject>amplified fragment length polymorphism</subject><subject>Animals</subject><subject>Animals, Domestic - microbiology</subject><subject>Biological and medical sciences</subject><subject>Campylobacter Infections - epidemiology</subject><subject>Campylobacter Infections - genetics</subject><subject>Campylobacter Infections - transmission</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - isolation & purification</subject><subject>campylobacteriosis</subject><subject>Cat Diseases - epidemiology</subject><subject>Cat Diseases - genetics</subject><subject>Cat Diseases - transmission</subject><subject>Cats</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - genetics</subject><subject>Cattle Diseases - transmission</subject><subject>Cluster Analysis</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - genetics</subject><subject>Dog Diseases - transmission</subject><subject>Dogs</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>host specificity</subject><subject>Humans</subject><subject>Microbiology</subject><subject>mixed infection</subject><subject>molecular epidemiology</subject><subject>pets</subject><subject>Polymorphism, Genetic - genetics</subject><subject>Poultry</subject><subject>Poultry Diseases - epidemiology</subject><subject>Poultry Diseases - genetics</subject><subject>Poultry Diseases - transmission</subject><subject>source identification</subject><subject>zoonoses</subject><subject>Zoonoses - epidemiology</subject><subject>Zoonoses - transmission</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEoqXwF8BCKrcEj-OP5MChWvGpIpCgZ8txJltHSbzYSdv99zjdFZW4gGXJI_t5Zzx-nWUEaAFpvO0LKKXImVSsYJSKgjJVVcXdo-z0z8Hj-5jngip2kj2LsacUSirk0-wEJE8RrU6zm-_XOPmJ2GGJMwZiJjPso4vEpElGnK99S2ZP3HSDcXZbMyPBnWtxdH7wW2fNQAIOabudMEbS4HyLOJHol2AxEt-RjRl3-8E3xq4FeuyXyT3PnnRmiPjiuJ5lVx_e_9x8yi-_ffy8ubjMrWB1ldfG0LKhqmkBLFppO4NU8o7zugOoWsFbUVvOZSOtqTolqTHAhQUmJTQllmfZm0PeXfC_ltSBHl20OAxmQr9ErahUSV7-EwQFTACvEvj6L7BPraZXi5qVrOYASiWoOkA2-BgDdnoX3GjCXgPVq4O616tRejVKrw7qewf1XZK-POZfmhHbB-HRsgScHwET0-t3wUzWxQdOCaU41Il7d-Bu3YD7_76A_nLxdY2S_tVB3xmvzTakGlc_GAWRvhEVNajyN_FqwV8</recordid><startdate>200602</startdate><enddate>200602</enddate><creator>Wieland, B</creator><creator>Wittwer, M</creator><creator>Regula, G</creator><creator>Wassenaar, T.M</creator><creator>Burnens, A.P</creator><creator>Keller, J</creator><creator>Stärk, K.D.C</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200602</creationdate><title>Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni</title><author>Wieland, B ; Wittwer, M ; Regula, G ; Wassenaar, T.M ; Burnens, A.P ; Keller, J ; Stärk, K.D.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5298-9aa03b07bd11cec6cfae064f449f118d54d59c446b6ca8f760aa145c12661b3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>amplified fragment length polymorphism</topic><topic>Animals</topic><topic>Animals, Domestic - microbiology</topic><topic>Biological and medical sciences</topic><topic>Campylobacter Infections - epidemiology</topic><topic>Campylobacter Infections - genetics</topic><topic>Campylobacter Infections - transmission</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - isolation & purification</topic><topic>campylobacteriosis</topic><topic>Cat Diseases - epidemiology</topic><topic>Cat Diseases - genetics</topic><topic>Cat Diseases - transmission</topic><topic>Cats</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - genetics</topic><topic>Cattle Diseases - transmission</topic><topic>Cluster Analysis</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - genetics</topic><topic>Dog Diseases - transmission</topic><topic>Dogs</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>host specificity</topic><topic>Humans</topic><topic>Microbiology</topic><topic>mixed infection</topic><topic>molecular epidemiology</topic><topic>pets</topic><topic>Polymorphism, Genetic - genetics</topic><topic>Poultry</topic><topic>Poultry Diseases - epidemiology</topic><topic>Poultry Diseases - genetics</topic><topic>Poultry Diseases - transmission</topic><topic>source identification</topic><topic>zoonoses</topic><topic>Zoonoses - epidemiology</topic><topic>Zoonoses - transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wieland, B</creatorcontrib><creatorcontrib>Wittwer, M</creatorcontrib><creatorcontrib>Regula, G</creatorcontrib><creatorcontrib>Wassenaar, T.M</creatorcontrib><creatorcontrib>Burnens, A.P</creatorcontrib><creatorcontrib>Keller, J</creatorcontrib><creatorcontrib>Stärk, K.D.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wieland, B</au><au>Wittwer, M</au><au>Regula, G</au><au>Wassenaar, T.M</au><au>Burnens, A.P</au><au>Keller, J</au><au>Stärk, K.D.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2006-02</date><risdate>2006</risdate><volume>100</volume><issue>2</issue><spage>316</spage><epage>324</epage><pages>316-324</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>To develop a method for assessing the relative epidemiological significance of possible infection sources for human campylobacteriosis. Using fluorescent amplified fragment length polymorphism (AFLP), 243 apparently epidemiologically unrelated Campylobacter jejuni isolates were genotyped (77 human, 46 cattle, 49 pet and 71 poultry isolates). In total 136 different phena were identified, of which 48 were clusters grouping at least two isolates. Isolates from different sources were frequently clustered together, underlining the high degree of source mixing and the lack of host specificity of C. jejuni. The phena were classified into different phenon types according to the sources of the isolates they contained. The occurrence of these phenon types was analysed using an area-proportional Euler diagram to describe epidemiological relatedness among C. jejuni isolates. Group separation statistics revealed that 43% of analysed human isolates expressed maximum similarity to other human isolates, 9% to cattle isolates, 21% to pet isolates and 27% to poultry isolates; these results were in accordance with the pattern observed in the phenon cluster analysis. Based on the grouping of strains into molecular similarity clusters, ecological patterns between sources can be investigated. This approach is a new methodological contribution to establish the relative epidemiological significance of concurrent infection sources.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16430508</pmid><doi>10.1111/j.1365-2672.2005.02788.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | amplified fragment length polymorphism Animals Animals, Domestic - microbiology Biological and medical sciences Campylobacter Infections - epidemiology Campylobacter Infections - genetics Campylobacter Infections - transmission Campylobacter jejuni Campylobacter jejuni - isolation & purification campylobacteriosis Cat Diseases - epidemiology Cat Diseases - genetics Cat Diseases - transmission Cats Cattle Cattle Diseases - epidemiology Cattle Diseases - genetics Cattle Diseases - transmission Cluster Analysis Dog Diseases - epidemiology Dog Diseases - genetics Dog Diseases - transmission Dogs fluorescence Fundamental and applied biological sciences. Psychology Genotype host specificity Humans Microbiology mixed infection molecular epidemiology pets Polymorphism, Genetic - genetics Poultry Poultry Diseases - epidemiology Poultry Diseases - genetics Poultry Diseases - transmission source identification zoonoses Zoonoses - epidemiology Zoonoses - transmission |
| title | Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni |
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