Isolation of Endothelial Cells from Brain, Lung, and Kidney: Expression of the Multidrug Resistance P-Glycoprotein Isoforms

Endothelial cells (EC) were isolated from brain, lung, and renal cortex using magnetic microbeads cross-linked to an antibody directed against the platelet-endothelial cell adhesion molecule-1 (PECAM-1). Levels of endothelial nitric oxide synthase (eNOS) and PECAM-1 were measured by Western blots an...

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Veröffentlicht in:	Biochemical and biophysical research communications 2001-03, Vol.281 (3), p.827-834
Hauptverfasser:	Demeule, Michel, Labelle, Myriam, Régina, Anthony, Berthelet, France, Béliveau, Richard
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals astrocytes ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism brain Brain - cytology Brain - metabolism endothelial cells Endothelium - cytology Endothelium - metabolism eNOS GFAP Immunomagnetic Separation kidney Kidney - cytology Kidney - metabolism lung Lung - cytology Lung - metabolism MDR P-glycoprotein PECAM-1 Platelet Endothelial Cell Adhesion Molecule-1 - immunology Protein Isoforms - metabolism Rats Reverse Transcriptase Polymerase Chain Reaction
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