Culture and fusion of pollen protoplasts of Brassica oleracea L. var. italica with haploid mesophyll protoplasts of B. rapa L. ssp. pekinensis

Hybrid callus was formed from the successful protoplast fusion between pollen protoplasts of Brassica oleracea var. italica and haploid mesophyll protoplasts of Brassica rapa. The pollen protoplast isolation frequency in broccoli was highly related to the ratio of trinucleate pollens in the male gam...

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Veröffentlicht in:	Protoplasma 2007-07, Vol.231 (1-2), p.89-97
Hauptverfasser:	Liu, Fan, Ryschka, U, Marthe, F, Klocke, E, Schumann, G, Zhao, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Alginic acid Brassica - classification Brassica - cytology Brassica oleracea Brassica rapa Callus Cell culture Cell Division Cell Fusion Cells, Cultured Centrifugation DNA, Plant Embedding Flowers & plants Fusion Haploid mesophyll protoplast Haploidy Hybrid callus Hybridization, Genetic Mesophyll Osmotic pressure Pollen Pollen - cytology Pollen protoplast Polyethylene glycol Primordia Protoplast fusion Protoplasts Protoplasts - cytology Random Amplified Polymorphic DNA Technique
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