Analytical methods for assay of ellagic acid and its solubility studies
Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted u...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2006-01, Vol.40 (1), p.206-210 |
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| creator | Bala, I. Bhardwaj, V. Hariharan, S. Kumar, M.N.V. Ravi |
| description | Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5
mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1
μg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and
N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively. |
| doi_str_mv | 10.1016/j.jpba.2005.07.006 |
| format | Article |
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mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1
μg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and
N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2005.07.006</identifier><identifier>PMID: 16111850</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical method ; Analytical, structural and metabolic biochemistry ; Antioxidants ; Antioxidants - analysis ; Biological and medical sciences ; Chemistry Techniques, Analytical - methods ; Chemistry, Pharmaceutical - methods ; Chromatography, High Pressure Liquid ; Dosage Forms ; Ellagic acid ; Ellagic Acid - analysis ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC ; Hydrogen-Ion Concentration ; Medical sciences ; Models, Chemical ; Pharmaceutical Preparations ; Pharmacology. Drug treatments ; Reproducibility of Results ; Sensitivity and Specificity ; Solubility ; Solvents - analysis ; Spectrophotometry, Ultraviolet ; Thermodynamics ; Time Factors ; Ultraviolet Rays ; Validation ; Water</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2006-01, Vol.40 (1), p.206-210</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-36b1de615894b4f754f31bde966d7bbd01c33945613b609b68336f748b2ba95e3</citedby><cites>FETCH-LOGICAL-c450t-36b1de615894b4f754f31bde966d7bbd01c33945613b609b68336f748b2ba95e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708505004371$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17508288$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16111850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bala, I.</creatorcontrib><creatorcontrib>Bhardwaj, V.</creatorcontrib><creatorcontrib>Hariharan, S.</creatorcontrib><creatorcontrib>Kumar, M.N.V. Ravi</creatorcontrib><title>Analytical methods for assay of ellagic acid and its solubility studies</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5
mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1
μg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and
N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.</description><subject>Analysis</subject><subject>Analytical method</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Biological and medical sciences</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dosage Forms</subject><subject>Ellagic acid</subject><subject>Ellagic Acid - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC</subject><subject>Hydrogen-Ion Concentration</subject><subject>Medical sciences</subject><subject>Models, Chemical</subject><subject>Pharmaceutical Preparations</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Solubility</subject><subject>Solvents - analysis</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Thermodynamics</subject><subject>Time Factors</subject><subject>Ultraviolet Rays</subject><subject>Validation</subject><subject>Water</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90D1r3EAQgOElJNgXx38gRdgm6aTMaLUfgjTGJLbBkCYGd8t-KdlDJ112JMP9--i4A3eppnlmGF7GPiLUCKi-buvt3ru6AZA16BpAvWEbNFpUjWqf37INaIGVBiMv2XuiLawQu_aCXaJCRCNhw-5uRjcc5hzcwHdp_jNF4v1UuCNyBz71PA2D-50DdyFH7sbI80ycpmHxecjzgdO8xJzoA3vXu4HS9Xlesacf33_d3lePP-8ebm8eq9BKmCuhPMakUJqu9W2vZdsL9DF1SkXtfQQMQnStVCi8gs4rI4TqdWt8410nk7hiX05392X6uySa7S5TOD45pmkhq0HpBk23wuYEQ5mISurtvuSdKweLYI_57NYe89ljPgvarvnWpU_n64vfpfi6cu61gs9n4GhN1hc3hkyvTkswjTGr-3ZyaW3xklOxFHIaQ4q5pDDbOOX__fEP4E6NFQ</recordid><startdate>20060123</startdate><enddate>20060123</enddate><creator>Bala, I.</creator><creator>Bhardwaj, V.</creator><creator>Hariharan, S.</creator><creator>Kumar, M.N.V. Ravi</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060123</creationdate><title>Analytical methods for assay of ellagic acid and its solubility studies</title><author>Bala, I. ; Bhardwaj, V. ; Hariharan, S. ; Kumar, M.N.V. Ravi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-36b1de615894b4f754f31bde966d7bbd01c33945613b609b68336f748b2ba95e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis</topic><topic>Analytical method</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Antioxidants</topic><topic>Antioxidants - analysis</topic><topic>Biological and medical sciences</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dosage Forms</topic><topic>Ellagic acid</topic><topic>Ellagic Acid - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC</topic><topic>Hydrogen-Ion Concentration</topic><topic>Medical sciences</topic><topic>Models, Chemical</topic><topic>Pharmaceutical Preparations</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Solubility</topic><topic>Solvents - analysis</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Thermodynamics</topic><topic>Time Factors</topic><topic>Ultraviolet Rays</topic><topic>Validation</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bala, I.</creatorcontrib><creatorcontrib>Bhardwaj, V.</creatorcontrib><creatorcontrib>Hariharan, S.</creatorcontrib><creatorcontrib>Kumar, M.N.V. 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Ravi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analytical methods for assay of ellagic acid and its solubility studies</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2006-01-23</date><risdate>2006</risdate><volume>40</volume><issue>1</issue><spage>206</spage><epage>210</epage><pages>206-210</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5
mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1
μg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and
N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16111850</pmid><doi>10.1016/j.jpba.2005.07.006</doi><tpages>5</tpages></addata></record> |
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| subjects | Analysis Analytical method Analytical, structural and metabolic biochemistry Antioxidants Antioxidants - analysis Biological and medical sciences Chemistry Techniques, Analytical - methods Chemistry, Pharmaceutical - methods Chromatography, High Pressure Liquid Dosage Forms Ellagic acid Ellagic Acid - analysis Fundamental and applied biological sciences. Psychology General pharmacology HPLC Hydrogen-Ion Concentration Medical sciences Models, Chemical Pharmaceutical Preparations Pharmacology. Drug treatments Reproducibility of Results Sensitivity and Specificity Solubility Solvents - analysis Spectrophotometry, Ultraviolet Thermodynamics Time Factors Ultraviolet Rays Validation Water |
| title | Analytical methods for assay of ellagic acid and its solubility studies |
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