Integration of active human β-galactosidase gene (100 kb) into genome using HSV/AAV amplicon vector

Vectors based on herpes simplex virus type-1 (HSV-1) permit delivery of transgenes of up to 150 kb, while the inverted terminal repeats and Rep of the adeno-associated virus (AAV) can confer site-specific integration into the AAVS1 site, which allows sustained expression of a transgene. In this stud...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Gene therapy 2007-07, Vol.14 (14), p.1078-1091
Hauptverfasser:	Oehmig, A, Cortés, M L, Perry, K F, Sena-Esteves, M, Fraefel, Cornel, Breakefield, X O
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Applied cell therapy and gene therapy beta-Galactosidase - analysis beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences Biomedical and Life Sciences Biomedicine Biotechnology Blotting, Southern - methods Cell Biology Cell Line, Transformed Cercopithecus aethiops Cloning Dependovirus - genetics Design and construction Errors of metabolism Expression vectors Fibroblasts - virology Fundamental and applied biological sciences. Psychology Gangliosidosis, GM1 - therapy Gene Expression Gene Therapy Genetic aspects Genetic Engineering Genetic Therapy - methods Genetic vectors Genetic Vectors - administration & dosage Genetic Vectors - genetics Genome Glioblastoma Green Fluorescent Proteins - genetics Health. Pharmaceutical industry Herpes simplex Herpesvirus 1, Human - genetics Human Genetics Humans Industrial applications and implications. Economical aspects Lipids (lysosomal enzyme disorders, storage diseases) Medical sciences Metabolic diseases Methods Nanotechnology original-article Physiological aspects Time Factors Transduction, Genetic - methods Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transgenes Virus Integration Viruses β-Galactosidase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!