Cutting Edge: Identification of E-Cadherin as a Ligand for the Murine Killer Cell Lectin-Like Receptor G1
The killer cell lectin-like receptor G1 (KLRG1) is expressed by NK cells and by T cells. In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a l...
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| Veröffentlicht in: | The Journal of immunology (1950) 2006-02, Vol.176 (3), p.1311-1315 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 176 |
| creator | Grundemann, Carsten Bauer, Monika Schweier, Oliver von Oppen, Nanette Lassing, Ute Saudan, Philippe Becker, Karl-Friedrich Karp, Klaus Hanke, Thomas Bachmann, Martin F Pircher, Hanspeter |
| description | The killer cell lectin-like receptor G1 (KLRG1) is expressed by NK cells and by T cells. In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. KLRG1 ligation by E-cadherin in healthy tissue may thus exert an inhibitory effect on primed T cells. |
| doi_str_mv | 10.4049/jimmunol.176.3.1311 |
| format | Article |
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In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. 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In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. KLRG1 ligation by E-cadherin in healthy tissue may thus exert an inhibitory effect on primed T cells.</description><subject>Animals</subject><subject>Antigens - immunology</subject><subject>Cadherins - biosynthesis</subject><subject>Cadherins - metabolism</subject><subject>Cadherins - physiology</subject><subject>CD8-Positive T-Lymphocytes - cytology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell Division - immunology</subject><subject>Cell Line</subject><subject>Growth Inhibitors - biosynthesis</subject><subject>Growth Inhibitors - metabolism</subject><subject>Growth Inhibitors - physiology</subject><subject>Killer Cells, Lymphokine-Activated - immunology</subject><subject>Killer Cells, Lymphokine-Activated - metabolism</subject><subject>Ligands</subject><subject>Melanoma, Experimental</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, Immunologic - metabolism</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LAzEQhoMotn78AkFy0tPWZJPdjd5kqVVcEUTPIU0mbep-1GSX4r83pRXNZSDzzMvMg9AFJRNO-O3NyjXN0Hb1hBb5hE0oo_QAjWmWkSTPSX6IxoSkaRK7xQidhLAihOQk5cdoRHOe8kiOkSuHvnftAk_NAu7wk4G2d9Zp1buuxZ3F06RUZgnetVgFrHDlFqo12HYe90vAL0PsAH52dQ0el1DXuAIdE5PKfQJ-Aw3rPrIzeoaOrKoDnO_rKfp4mL6Xj0n1Onsq76tEc573iVZZRo0VyhZE6NTEX6sFo9pyXVgAroUQzKhM3zKRCaVIZrJUmbmIRzEl2Cm62uWuffc1QOhl44KOi6kWuiHIguTxCRpBtgO170LwYOXau0b5b0mJ3BqWv4ZldCiZ3BqOU5f7-GHegPmb2SuNwPUOWLrFcuM8yNCouo44lZvN5l_UD52ChqA</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Grundemann, Carsten</creator><creator>Bauer, Monika</creator><creator>Schweier, Oliver</creator><creator>von Oppen, Nanette</creator><creator>Lassing, Ute</creator><creator>Saudan, Philippe</creator><creator>Becker, Karl-Friedrich</creator><creator>Karp, Klaus</creator><creator>Hanke, Thomas</creator><creator>Bachmann, Martin F</creator><creator>Pircher, Hanspeter</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060201</creationdate><title>Cutting Edge: Identification of E-Cadherin as a Ligand for the Murine Killer Cell Lectin-Like Receptor G1</title><author>Grundemann, Carsten ; 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In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antigens - immunology Cadherins - biosynthesis Cadherins - metabolism Cadherins - physiology CD8-Positive T-Lymphocytes - cytology CD8-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - metabolism Cell Division - immunology Cell Line Growth Inhibitors - biosynthesis Growth Inhibitors - metabolism Growth Inhibitors - physiology Killer Cells, Lymphokine-Activated - immunology Killer Cells, Lymphokine-Activated - metabolism Ligands Melanoma, Experimental Mice Mice, Inbred C57BL Mice, Transgenic Receptors, Immunologic - genetics Receptors, Immunologic - metabolism |
| title | Cutting Edge: Identification of E-Cadherin as a Ligand for the Murine Killer Cell Lectin-Like Receptor G1 |
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