Angiotensin II and Its Receptor Subtypes in the Human Retina
To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina. Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina an...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2007-07, Vol.48 (7), p.3301-3311 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Senanayake, Preenie deS Drazba, Judy Shadrach, Karen Milsted, Amy Rungger-Brandle, Elisabeth Nishiyama, Kazutoshi Miura, Shin-Ichiro Karnik, Sadashiva Sears, Jonathan E Hollyfield, Joe G |
| description | To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina.
Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina and vitreous was quantified by RIA. Ang II receptors were characterized and quantified by competitive membrane-binding assays. Ang II, its heptapeptide metabolite Ang-(1-7), and AT1 and AT2 receptors were localized by immunohistochemistry and confocal imaging.
Levels of Ang II in the retina were significantly higher than in vitreous (P < 0.05). Ang II in the diabetic retina had a higher median compared with that in the nondiabetic retina. Ang II and Ang-(1-7) colocalized in retinal Müller cells. The retina had the highest levels of Ang II receptors that were significantly higher than the optic nerve, retinal pigment epithelium-choroid complex, and ciliary body-iris complex (P < 0.05). AT1 receptors were more abundant than AT2 receptors in the retina. Immunoreactivity for AT1 was detected in Müller cells and on blood vessels. AT2 receptors were localized throughout the Müller cells and nuclei of ganglion cells and neurons in the inner nuclear layer.
In the human retina, identification of Ang II and its bioactive metabolite Ang-(1-7) in Müller cells suggests that these glial cells are able to produce and process Ang II. Ang receptors were localized in the blood vessels and neural cells. Local Ang II signaling may thus allow for autoregulation of neurovascular activity. Such an autonomous system could modulate the onset and severity of retinovascular disease. |
| doi_str_mv | 10.1167/iovs.06-1024 |
| format | Article |
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Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina and vitreous was quantified by RIA. Ang II receptors were characterized and quantified by competitive membrane-binding assays. Ang II, its heptapeptide metabolite Ang-(1-7), and AT1 and AT2 receptors were localized by immunohistochemistry and confocal imaging.
Levels of Ang II in the retina were significantly higher than in vitreous (P < 0.05). Ang II in the diabetic retina had a higher median compared with that in the nondiabetic retina. Ang II and Ang-(1-7) colocalized in retinal Müller cells. The retina had the highest levels of Ang II receptors that were significantly higher than the optic nerve, retinal pigment epithelium-choroid complex, and ciliary body-iris complex (P < 0.05). AT1 receptors were more abundant than AT2 receptors in the retina. Immunoreactivity for AT1 was detected in Müller cells and on blood vessels. AT2 receptors were localized throughout the Müller cells and nuclei of ganglion cells and neurons in the inner nuclear layer.
In the human retina, identification of Ang II and its bioactive metabolite Ang-(1-7) in Müller cells suggests that these glial cells are able to produce and process Ang II. Ang receptors were localized in the blood vessels and neural cells. Local Ang II signaling may thus allow for autoregulation of neurovascular activity. Such an autonomous system could modulate the onset and severity of retinovascular disease.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.06-1024</identifier><identifier>PMID: 17591902</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Angiotensin I - metabolism ; Angiotensin II - metabolism ; Binding, Competitive ; Biological and medical sciences ; Blotting, Western ; Eye and associated structures. Visual pathways and centers. Vision ; Female ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Microscopy, Confocal ; Middle Aged ; Peptide Fragments - metabolism ; Peptidyl-Dipeptidase A - metabolism ; Radioimmunoassay ; Receptor, Angiotensin, Type 1 - metabolism ; Receptor, Angiotensin, Type 2 - metabolism ; Retina - metabolism ; Tissue Donors ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 2007-07, Vol.48 (7), p.3301-3311</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-c6d3f7b3ab6ebdd76fc2cf926fd3ad04b3801cbf8c93995dd054b5574392cc303</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18897687$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17591902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Senanayake, Preenie deS</creatorcontrib><creatorcontrib>Drazba, Judy</creatorcontrib><creatorcontrib>Shadrach, Karen</creatorcontrib><creatorcontrib>Milsted, Amy</creatorcontrib><creatorcontrib>Rungger-Brandle, Elisabeth</creatorcontrib><creatorcontrib>Nishiyama, Kazutoshi</creatorcontrib><creatorcontrib>Miura, Shin-Ichiro</creatorcontrib><creatorcontrib>Karnik, Sadashiva</creatorcontrib><creatorcontrib>Sears, Jonathan E</creatorcontrib><creatorcontrib>Hollyfield, Joe G</creatorcontrib><title>Angiotensin II and Its Receptor Subtypes in the Human Retina</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina.
Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina and vitreous was quantified by RIA. Ang II receptors were characterized and quantified by competitive membrane-binding assays. Ang II, its heptapeptide metabolite Ang-(1-7), and AT1 and AT2 receptors were localized by immunohistochemistry and confocal imaging.
Levels of Ang II in the retina were significantly higher than in vitreous (P < 0.05). Ang II in the diabetic retina had a higher median compared with that in the nondiabetic retina. Ang II and Ang-(1-7) colocalized in retinal Müller cells. The retina had the highest levels of Ang II receptors that were significantly higher than the optic nerve, retinal pigment epithelium-choroid complex, and ciliary body-iris complex (P < 0.05). AT1 receptors were more abundant than AT2 receptors in the retina. Immunoreactivity for AT1 was detected in Müller cells and on blood vessels. AT2 receptors were localized throughout the Müller cells and nuclei of ganglion cells and neurons in the inner nuclear layer.
In the human retina, identification of Ang II and its bioactive metabolite Ang-(1-7) in Müller cells suggests that these glial cells are able to produce and process Ang II. Ang receptors were localized in the blood vessels and neural cells. Local Ang II signaling may thus allow for autoregulation of neurovascular activity. Such an autonomous system could modulate the onset and severity of retinovascular disease.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Angiotensin I - metabolism</subject><subject>Angiotensin II - metabolism</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Middle Aged</subject><subject>Peptide Fragments - metabolism</subject><subject>Peptidyl-Dipeptidase A - metabolism</subject><subject>Radioimmunoassay</subject><subject>Receptor, Angiotensin, Type 1 - metabolism</subject><subject>Receptor, Angiotensin, Type 2 - metabolism</subject><subject>Retina - metabolism</subject><subject>Tissue Donors</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LwzAYh4Mobk5vnqUXPdn55qNJA17GUFcYCH6cQ5qkW6Qfs2kd--_t2GCn9_B7eHh5ELrFMMWYiyff_IUp8BgDYWdojJOExIlI6TkaA2Y8BgZshK5C-AEgGBO4RCMsEoklkDF6ntUr33SuDr6OsizStY2yLkQfzrhN17TRZ593u40L0bB3axct-krXw9z5Wl-ji0KXwd0c7wR9v758zRfx8v0tm8-WsWGYd7HhlhYipzrnLrdW8MIQU0jCC0u1BZbTFLDJi9RIKmViLSQsTxLBqCTGUKAT9HDwbtrmt3ehU5UPxpWlrl3TByWAc8KlHMDHA2jaJoTWFWrT-kq3O4VB7WupfS0FXO1rDfjd0dvnlbMn-JhnAO6PgA5Gl0Wra-PDiUtTKXgqTg-u_Wq99a1TodJlOWix2m63LFVCUQqY_gMG-H9O</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Senanayake, Preenie deS</creator><creator>Drazba, Judy</creator><creator>Shadrach, Karen</creator><creator>Milsted, Amy</creator><creator>Rungger-Brandle, Elisabeth</creator><creator>Nishiyama, Kazutoshi</creator><creator>Miura, Shin-Ichiro</creator><creator>Karnik, Sadashiva</creator><creator>Sears, Jonathan E</creator><creator>Hollyfield, Joe G</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Angiotensin II and Its Receptor Subtypes in the Human Retina</title><author>Senanayake, Preenie deS ; Drazba, Judy ; Shadrach, Karen ; Milsted, Amy ; Rungger-Brandle, Elisabeth ; Nishiyama, Kazutoshi ; Miura, Shin-Ichiro ; Karnik, Sadashiva ; Sears, Jonathan E ; Hollyfield, Joe G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-c6d3f7b3ab6ebdd76fc2cf926fd3ad04b3801cbf8c93995dd054b5574392cc303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Angiotensin I - metabolism</topic><topic>Angiotensin II - metabolism</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Middle Aged</topic><topic>Peptide Fragments - metabolism</topic><topic>Peptidyl-Dipeptidase A - metabolism</topic><topic>Radioimmunoassay</topic><topic>Receptor, Angiotensin, Type 1 - metabolism</topic><topic>Receptor, Angiotensin, Type 2 - metabolism</topic><topic>Retina - metabolism</topic><topic>Tissue Donors</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Senanayake, Preenie deS</creatorcontrib><creatorcontrib>Drazba, Judy</creatorcontrib><creatorcontrib>Shadrach, Karen</creatorcontrib><creatorcontrib>Milsted, Amy</creatorcontrib><creatorcontrib>Rungger-Brandle, Elisabeth</creatorcontrib><creatorcontrib>Nishiyama, Kazutoshi</creatorcontrib><creatorcontrib>Miura, Shin-Ichiro</creatorcontrib><creatorcontrib>Karnik, Sadashiva</creatorcontrib><creatorcontrib>Sears, Jonathan E</creatorcontrib><creatorcontrib>Hollyfield, Joe G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Senanayake, Preenie deS</au><au>Drazba, Judy</au><au>Shadrach, Karen</au><au>Milsted, Amy</au><au>Rungger-Brandle, Elisabeth</au><au>Nishiyama, Kazutoshi</au><au>Miura, Shin-Ichiro</au><au>Karnik, Sadashiva</au><au>Sears, Jonathan E</au><au>Hollyfield, Joe G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin II and Its Receptor Subtypes in the Human Retina</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>48</volume><issue>7</issue><spage>3301</spage><epage>3311</epage><pages>3301-3311</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To quantify and evaluate the distribution of angiotensin II (Ang II) and its receptors in the human retina.
Donor eyes were obtained within 12 hours postmortem and classified as hypertensive or normotensive and diabetic or nondiabetic, based on the donors' medical histories. Ang II in retina and vitreous was quantified by RIA. Ang II receptors were characterized and quantified by competitive membrane-binding assays. Ang II, its heptapeptide metabolite Ang-(1-7), and AT1 and AT2 receptors were localized by immunohistochemistry and confocal imaging.
Levels of Ang II in the retina were significantly higher than in vitreous (P < 0.05). Ang II in the diabetic retina had a higher median compared with that in the nondiabetic retina. Ang II and Ang-(1-7) colocalized in retinal Müller cells. The retina had the highest levels of Ang II receptors that were significantly higher than the optic nerve, retinal pigment epithelium-choroid complex, and ciliary body-iris complex (P < 0.05). AT1 receptors were more abundant than AT2 receptors in the retina. Immunoreactivity for AT1 was detected in Müller cells and on blood vessels. AT2 receptors were localized throughout the Müller cells and nuclei of ganglion cells and neurons in the inner nuclear layer.
In the human retina, identification of Ang II and its bioactive metabolite Ang-(1-7) in Müller cells suggests that these glial cells are able to produce and process Ang II. Ang receptors were localized in the blood vessels and neural cells. Local Ang II signaling may thus allow for autoregulation of neurovascular activity. Such an autonomous system could modulate the onset and severity of retinovascular disease.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>17591902</pmid><doi>10.1167/iovs.06-1024</doi><tpages>11</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Angiotensin I - metabolism Angiotensin II - metabolism Binding, Competitive Biological and medical sciences Blotting, Western Eye and associated structures. Visual pathways and centers. Vision Female Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Humans Male Microscopy, Confocal Middle Aged Peptide Fragments - metabolism Peptidyl-Dipeptidase A - metabolism Radioimmunoassay Receptor, Angiotensin, Type 1 - metabolism Receptor, Angiotensin, Type 2 - metabolism Retina - metabolism Tissue Donors Vertebrates: nervous system and sense organs |
| title | Angiotensin II and Its Receptor Subtypes in the Human Retina |
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