Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses
Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicostephanus longibursatus, Cylicostephanus goldi and a...
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| Veröffentlicht in: | International journal for parasitology 2001-02, Vol.31 (2), p.197-204 |
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| container_title | International journal for parasitology |
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| creator | Hodgkinson, J.E. Love, S. Lichtenfels, J.R. Palfreman, S. Ramsey, Y.H. Matthews, J.B. |
| description | Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of
Cylicocyclus ashworthi,
Cylicocyclus nassatus,
Cylicostephanus longibursatus,
Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus
Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes. |
| doi_str_mv | 10.1016/S0020-7519(00)00161-2 |
| format | Article |
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Cylicocyclus ashworthi,
Cylicocyclus nassatus,
Cylicostephanus longibursatus,
Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus
Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(00)00161-2</identifier><identifier>PMID: 11239940</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cyathostominae ; DNA, Helminth - analysis ; DNA, Helminth - genetics ; DNA, Ribosomal Spacer - analysis ; DNA, Ribosomal Spacer - genetics ; Fundamental and applied biological sciences. Psychology ; Horse Diseases - parasitology ; Horses ; Intergenic spacer region ; Invertebrates ; Molecular Sequence Data ; Nemathelminthia. Plathelmintha ; Nematoda ; Oligonucleotide Probes ; Polymerase Chain Reaction - methods ; Sequence Alignment ; Sequence Analysis, DNA ; Species Specificity ; Species-specific DNA probes ; Strongylida Infections - parasitology ; Strongylida Infections - veterinary ; Strongyloidea - classification ; Strongyloidea - genetics ; Strongyloidea - isolation & purification ; Systematics. Geographical distribution</subject><ispartof>International journal for parasitology, 2001-02, Vol.31 (2), p.197-204</ispartof><rights>2001 Australian Society for Parasitology Inc</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-a026125c128d36c208830e9fab75cd0645ad35c5486305e3f7447e7a646755093</citedby><cites>FETCH-LOGICAL-c389t-a026125c128d36c208830e9fab75cd0645ad35c5486305e3f7447e7a646755093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0020-7519(00)00161-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=964501$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11239940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hodgkinson, J.E.</creatorcontrib><creatorcontrib>Love, S.</creatorcontrib><creatorcontrib>Lichtenfels, J.R.</creatorcontrib><creatorcontrib>Palfreman, S.</creatorcontrib><creatorcontrib>Ramsey, Y.H.</creatorcontrib><creatorcontrib>Matthews, J.B.</creatorcontrib><title>Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of
Cylicocyclus ashworthi,
Cylicocyclus nassatus,
Cylicostephanus longibursatus,
Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus
Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cyathostominae</subject><subject>DNA, Helminth - analysis</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Ribosomal Spacer - analysis</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horse Diseases - parasitology</subject><subject>Horses</subject><subject>Intergenic spacer region</subject><subject>Invertebrates</subject><subject>Molecular Sequence Data</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Nematoda</subject><subject>Oligonucleotide Probes</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Species-specific DNA probes</subject><subject>Strongylida Infections - parasitology</subject><subject>Strongylida Infections - veterinary</subject><subject>Strongyloidea - classification</subject><subject>Strongyloidea - genetics</subject><subject>Strongyloidea - isolation & purification</subject><subject>Systematics. Geographical distribution</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVoSRy3P6FloRCSw7ajz909hRCStBDooe1ZyNpRrbK7ciXZ4H8fbWzcY08Dw_POjB4R8oHCZwpUffkBwKBuJO2uAW6gtGjNzsiCtk1XA-XyDVmckAtymdKfAkkuxDm5oJTxrhOwIOuHnRm2JvswVcFVeY1V2qD1zluf93PL-R1WYfC_wyaGFabKhVj5Hqc8Q6ek3Zu8DimH0U-HETMaw1itQ0yY3pG3zgwJ3x_rkvx6fPh5_7V-_v707f7uuba87XJtgCnKpKWs7bmyDNqWA3bOrBppe1BCmp5LK0WrOEjkrhGiwcYooRopoeNLcnWYW679u8WU9eiTxWEwE4Zt0g0oxaSQBZQH0MaQUkSnN9GPJu41BT0r1q-K9exPA-hXxZqV3Mfjgu1qxP5f6ui0AJ-OgEnWDC6ayfp04rryhvI_S3J7oLDI2HmMOhVlk8XeR7RZ98H_55AXXC2X9w</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Hodgkinson, J.E.</creator><creator>Love, S.</creator><creator>Lichtenfels, J.R.</creator><creator>Palfreman, S.</creator><creator>Ramsey, Y.H.</creator><creator>Matthews, J.B.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses</title><author>Hodgkinson, J.E. ; Love, S. ; Lichtenfels, J.R. ; Palfreman, S. ; Ramsey, Y.H. ; Matthews, J.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-a026125c128d36c208830e9fab75cd0645ad35c5486305e3f7447e7a646755093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cyathostominae</topic><topic>DNA, Helminth - analysis</topic><topic>DNA, Helminth - genetics</topic><topic>DNA, Ribosomal Spacer - analysis</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Horse Diseases - parasitology</topic><topic>Horses</topic><topic>Intergenic spacer region</topic><topic>Invertebrates</topic><topic>Molecular Sequence Data</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Nematoda</topic><topic>Oligonucleotide Probes</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Species-specific DNA probes</topic><topic>Strongylida Infections - parasitology</topic><topic>Strongylida Infections - veterinary</topic><topic>Strongyloidea - classification</topic><topic>Strongyloidea - genetics</topic><topic>Strongyloidea - isolation & purification</topic><topic>Systematics. Geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hodgkinson, J.E.</creatorcontrib><creatorcontrib>Love, S.</creatorcontrib><creatorcontrib>Lichtenfels, J.R.</creatorcontrib><creatorcontrib>Palfreman, S.</creatorcontrib><creatorcontrib>Ramsey, Y.H.</creatorcontrib><creatorcontrib>Matthews, J.B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hodgkinson, J.E.</au><au>Love, S.</au><au>Lichtenfels, J.R.</au><au>Palfreman, S.</au><au>Ramsey, Y.H.</au><au>Matthews, J.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>31</volume><issue>2</issue><spage>197</spage><epage>204</epage><pages>197-204</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Here, we report evaluation of five oligoprobes designed from intergenic spacer (IGS) region sequences for identification of cyathostomin species. Oligoprobes were designed for identification of
Cylicocyclus ashworthi,
Cylicocyclus nassatus,
Cylicostephanus longibursatus,
Cylicostephanus goldi and a fifth probe designed to identify all members of this tribe. PCR amplification of IGS DNA from 16 cyathostomin species allowed sequence comparison and identification of four putative species-specific probes. Southern blotting of amplified products from 16 species showed that all probes were species-specific. The fifth probe recognised all 16 cyathostomin species but did not bind to members of the genus
Strongylus. Furthermore, these probes were used to identify individual infective L3, eggs and L4 indicating that they will be invaluable to furthering the study of the epidemiology and pathogenesis of these important equine nematodes.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11239940</pmid><doi>10.1016/S0020-7519(00)00161-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Biological and medical sciences Cyathostominae DNA, Helminth - analysis DNA, Helminth - genetics DNA, Ribosomal Spacer - analysis DNA, Ribosomal Spacer - genetics Fundamental and applied biological sciences. Psychology Horse Diseases - parasitology Horses Intergenic spacer region Invertebrates Molecular Sequence Data Nemathelminthia. Plathelmintha Nematoda Oligonucleotide Probes Polymerase Chain Reaction - methods Sequence Alignment Sequence Analysis, DNA Species Specificity Species-specific DNA probes Strongylida Infections - parasitology Strongylida Infections - veterinary Strongyloidea - classification Strongyloidea - genetics Strongyloidea - isolation & purification Systematics. Geographical distribution |
| title | Evaluation of the specificity of five oligoprobes for identification of cyathostomin species from horses |
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