Control of interferon-τ secretion by in vitro-derived bovine blastocysts during extended culture and outgrowth formation

A series of experiments was conducted to examine the pattern of interferon‐τ (IFN‐τ) secretion by bovine blastocysts during extended culture in vitro. In the first experiment, blastocysts were cultured individually for three 48‐hour periods. The day of blastocyst formation affected how much IFN‐τ wa...

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Veröffentlicht in:Molecular reproduction and development 2001-04, Vol.58 (4), p.390-397
Hauptverfasser: Kubisch, H. Michael, Larson, Melissa A., Kiesling, David O.
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Kiesling, David O.
description A series of experiments was conducted to examine the pattern of interferon‐τ (IFN‐τ) secretion by bovine blastocysts during extended culture in vitro. In the first experiment, blastocysts were cultured individually for three 48‐hour periods. The day of blastocyst formation affected how much IFN‐τ was produced during the first two culture periods, but not during the third period. The overall secretion of IFN‐τ during the 6‐day period increased significantly and well beyond what could be accounted for by the concomitant increase in cell numbers. In the second experiment, blastocysts were initially cultured in individual droplets for 48 hr, then plated into 48‐well plates. Medium concentrations of IFN‐τ were determined after 48 hr and again after 6 and 12 days of culture. Initial IFN‐τ secretion did not affect the ability to form outgrowths or their final size, and initial differences in secretion between groups of blastocysts had disappeared by the second and third analyses. In the third experiment, blastocysts were cultured individually for 48 hr in droplets containing the medium that had been flushed through the uteri of non‐pregnant sheep on days 10, 12, and 15 of the estrous cycle. Culture in the medium obtained from the Day 15 flush significantly increased the number of cells that blastocysts contained, as well as IFN‐τ secretion. Mol. Reprod. Dev. 58:390–397, 2001. © 2001 Wiley‐Liss, Inc.
doi_str_mv 10.1002/1098-2795(20010401)58:4<390::AID-MRD6>3.0.CO;2-V
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Initial IFN‐τ secretion did not affect the ability to form outgrowths or their final size, and initial differences in secretion between groups of blastocysts had disappeared by the second and third analyses. In the third experiment, blastocysts were cultured individually for 48 hr in droplets containing the medium that had been flushed through the uteri of non‐pregnant sheep on days 10, 12, and 15 of the estrous cycle. Culture in the medium obtained from the Day 15 flush significantly increased the number of cells that blastocysts contained, as well as IFN‐τ secretion. Mol. Reprod. 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Michael</creatorcontrib><creatorcontrib>Larson, Melissa A.</creatorcontrib><creatorcontrib>Kiesling, David O.</creatorcontrib><title>Control of interferon-τ secretion by in vitro-derived bovine blastocysts during extended culture and outgrowth formation</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>A series of experiments was conducted to examine the pattern of interferon‐τ (IFN‐τ) secretion by bovine blastocysts during extended culture in vitro. In the first experiment, blastocysts were cultured individually for three 48‐hour periods. The day of blastocyst formation affected how much IFN‐τ was produced during the first two culture periods, but not during the third period. The overall secretion of IFN‐τ during the 6‐day period increased significantly and well beyond what could be accounted for by the concomitant increase in cell numbers. In the second experiment, blastocysts were initially cultured in individual droplets for 48 hr, then plated into 48‐well plates. Medium concentrations of IFN‐τ were determined after 48 hr and again after 6 and 12 days of culture. Initial IFN‐τ secretion did not affect the ability to form outgrowths or their final size, and initial differences in secretion between groups of blastocysts had disappeared by the second and third analyses. In the third experiment, blastocysts were cultured individually for 48 hr in droplets containing the medium that had been flushed through the uteri of non‐pregnant sheep on days 10, 12, and 15 of the estrous cycle. Culture in the medium obtained from the Day 15 flush significantly increased the number of cells that blastocysts contained, as well as IFN‐τ secretion. Mol. Reprod. Dev. 58:390–397, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - metabolism</subject><subject>Cattle - embryology</subject><subject>Culture Media, Conditioned</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>embryo development</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Estrus</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gestational Age</subject><subject>in vitro fertilization</subject><subject>Interferon Type I - metabolism</subject><subject>Organ Culture Techniques</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - metabolism</subject><subject>Sheep - physiology</subject><subject>Therapeutic Irrigation</subject><subject>Time Factors</subject><subject>Uterus - chemistry</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkd1u0zAYQCMEYmPwCsgSEoKLlM9_sT0Q0tRBN6mjgKBwZzmJMzzSeNhOt97zfrwSCe3KlW35fOfiO1kmMUwwAHmFQcmcCMVfEAAMDPBLLo_ZG6rg-Pjk_DS_-HxavKUTmEwXr0m-vJcd7kfuj3cGOePk-0H2KMYrAFBKwsPsAGPCsBD8MNtMfZeCb5FvkOuSDY0Nvsv__EbRVsEm5ztUboYvtHYDl9c2uLWtUenXrrOobE1MvtrEFFHdB9ddInubbFcPSNW3qQ8Wma5Gvk-Xwd-kH6jxYWVG7ePsQWPaaJ_szqPs6_t3X6Zn-XwxO5-ezHNHmSjyWuK6wRJTBg1nJW0ks5VSvMZUcaUEp4aVrCJgy5IUVSmFYIKAwFJaJSSlR9nzrfc6-F-9jUmvXKxs25rO-j5qAQUXw9AAPt2Bfbmytb4ObmXCRt8tawCe7QATK9M2wXSVi3tOAWdUDdSnLXXjWrv5bwE9JtVjHz320XdJNZea6SGpHorqsaimGvR0oYle_nsPznzrdDHZ273ThJ-6EFRw_e3DTM-WrDj7OCf6gv4FrxyoqA</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Kubisch, H. 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Michael ; Larson, Melissa A. ; Kiesling, David O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3476-d81df181340f54b3f84ec995d139599753a4b4c20ebb26cb87747207188e97833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - metabolism</topic><topic>Cattle - embryology</topic><topic>Culture Media, Conditioned</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>embryo development</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Estrus</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gestational Age</topic><topic>in vitro fertilization</topic><topic>Interferon Type I - metabolism</topic><topic>Organ Culture Techniques</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - metabolism</topic><topic>Sheep - physiology</topic><topic>Therapeutic Irrigation</topic><topic>Time Factors</topic><topic>Uterus - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kubisch, H. Michael</creatorcontrib><creatorcontrib>Larson, Melissa A.</creatorcontrib><creatorcontrib>Kiesling, David O.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kubisch, H. Michael</au><au>Larson, Melissa A.</au><au>Kiesling, David O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control of interferon-τ secretion by in vitro-derived bovine blastocysts during extended culture and outgrowth formation</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>58</volume><issue>4</issue><spage>390</spage><epage>397</epage><pages>390-397</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>A series of experiments was conducted to examine the pattern of interferon‐τ (IFN‐τ) secretion by bovine blastocysts during extended culture in vitro. In the first experiment, blastocysts were cultured individually for three 48‐hour periods. The day of blastocyst formation affected how much IFN‐τ was produced during the first two culture periods, but not during the third period. The overall secretion of IFN‐τ during the 6‐day period increased significantly and well beyond what could be accounted for by the concomitant increase in cell numbers. In the second experiment, blastocysts were initially cultured in individual droplets for 48 hr, then plated into 48‐well plates. Medium concentrations of IFN‐τ were determined after 48 hr and again after 6 and 12 days of culture. Initial IFN‐τ secretion did not affect the ability to form outgrowths or their final size, and initial differences in secretion between groups of blastocysts had disappeared by the second and third analyses. In the third experiment, blastocysts were cultured individually for 48 hr in droplets containing the medium that had been flushed through the uteri of non‐pregnant sheep on days 10, 12, and 15 of the estrous cycle. Culture in the medium obtained from the Day 15 flush significantly increased the number of cells that blastocysts contained, as well as IFN‐τ secretion. Mol. Reprod. Dev. 58:390–397, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>11241775</pmid><doi>10.1002/1098-2795(20010401)58:4&lt;390::AID-MRD6&gt;3.0.CO;2-V</doi><tpages>8</tpages></addata></record>
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subjects Animals
Biological and medical sciences
Blastocyst - metabolism
Cattle - embryology
Culture Media, Conditioned
Early stages. Segmentation. Gastrulation. Neurulation
embryo development
Embryology: invertebrates and vertebrates. Teratology
Estrus
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
gene expression
Gestational Age
in vitro fertilization
Interferon Type I - metabolism
Organ Culture Techniques
Pregnancy
Pregnancy Proteins - metabolism
Sheep - physiology
Therapeutic Irrigation
Time Factors
Uterus - chemistry
title Control of interferon-τ secretion by in vitro-derived bovine blastocysts during extended culture and outgrowth formation
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