Mitochondria in human offspring derived from ooplasmic transplantation

Ooplasmic transfer from fertile donor oocytes into potentially compromised recipient patient oocytes has led to the birth of nearly 30 babies worldwide. Cytoplasmic transplantation has caused apprehension, since the mixing of human ooplasm from two different maternal sources may generate mitochondri...

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Veröffentlicht in:	Human reproduction (Oxford) 2001-03, Vol.16 (3), p.513-516
Hauptverfasser:	BARRITT, Jason A, BRENNER, Carol A, MALTER, Henry E, COHEN, Jacques
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Birth control Cytoplasm - physiology Cytoplasm - transplantation DNA Fingerprinting DNA, Mitochondrial Female Gynecology. Andrology. Obstetrics Humans Infant Medical sciences Microscopy, Confocal Mitochondria - physiology Mitochondria - ultrastructure Oocytes Sterility. Assisted procreation
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creator	BARRITT, Jason A BRENNER, Carol A MALTER, Henry E COHEN, Jacques
description	Ooplasmic transfer from fertile donor oocytes into potentially compromised recipient patient oocytes has led to the birth of nearly 30 babies worldwide. Cytoplasmic transplantation has caused apprehension, since the mixing of human ooplasm from two different maternal sources may generate mitochondrial (mt) heteroplasmy (both recipient and donor mtDNA) in offspring. This investigation traced the mitochondrial donor population both during the ooplasmic transfer technique and in the bloods of two 1 year old children using mtDNA fingerprinting. Donor ooplasm stained for active mitochondria was transferred into recipient ooplasm and the mitochondria were visualized by confocal microscopy after the microinjection procedure and fertilization. Heteroplasmy was found in the blood from each of the children. This report is the first case of human germline genetic modification resulting in normal healthy children.
doi_str_mv	10.1093/humrep/16.3.513
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Cytoplasmic transplantation has caused apprehension, since the mixing of human ooplasm from two different maternal sources may generate mitochondrial (mt) heteroplasmy (both recipient and donor mtDNA) in offspring. This investigation traced the mitochondrial donor population both during the ooplasmic transfer technique and in the bloods of two 1 year old children using mtDNA fingerprinting. Donor ooplasm stained for active mitochondria was transferred into recipient ooplasm and the mitochondria were visualized by confocal microscopy after the microinjection procedure and fertilization. Heteroplasmy was found in the blood from each of the children. 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Cytoplasmic transplantation has caused apprehension, since the mixing of human ooplasm from two different maternal sources may generate mitochondrial (mt) heteroplasmy (both recipient and donor mtDNA) in offspring. This investigation traced the mitochondrial donor population both during the ooplasmic transfer technique and in the bloods of two 1 year old children using mtDNA fingerprinting. Donor ooplasm stained for active mitochondria was transferred into recipient ooplasm and the mitochondria were visualized by confocal microscopy after the microinjection procedure and fertilization. Heteroplasmy was found in the blood from each of the children. This report is the first case of human germline genetic modification resulting in normal healthy children.</description><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cytoplasm - physiology</subject><subject>Cytoplasm - transplantation</subject><subject>DNA Fingerprinting</subject><subject>DNA, Mitochondrial</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Infant</subject><subject>Medical sciences</subject><subject>Microscopy, Confocal</subject><subject>Mitochondria - physiology</subject><subject>Mitochondria - ultrastructure</subject><subject>Oocytes</subject><subject>Sterility. 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source	Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals
subjects	Biological and medical sciences Birth control Cytoplasm - physiology Cytoplasm - transplantation DNA Fingerprinting DNA, Mitochondrial Female Gynecology. Andrology. Obstetrics Humans Infant Medical sciences Microscopy, Confocal Mitochondria - physiology Mitochondria - ultrastructure Oocytes Sterility. Assisted procreation
title	Mitochondria in human offspring derived from ooplasmic transplantation
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