Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
Noncoding RNAs (ncRNA) participate in epigenetic regulation but are poorly understood. Here we characterize the transcriptional landscape of the four human HOX loci at five base pair resolution in 11 anatomic sites and identify 231 HOX ncRNAs that extend known transcribed regions by more than 30 kil...
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| Veröffentlicht in: | Cell 2007-06, Vol.129 (7), p.1311-1323 |
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| container_title | Cell |
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| creator | Rinn, John L. Kertesz, Michael Wang, Jordon K. Squazzo, Sharon L. Xu, Xiao Brugmann, Samantha A. Goodnough, L. Henry Helms, Jill A. Farnham, Peggy J. Segal, Eran Chang, Howard Y. |
| description | Noncoding RNAs (ncRNA) participate in epigenetic regulation but are poorly understood. Here we characterize the transcriptional landscape of the four human
HOX loci at five base pair resolution in 11 anatomic sites and identify 231
HOX ncRNAs that extend known transcribed regions by more than 30 kilobases.
HOX ncRNAs are spatially expressed along developmental axes and possess unique sequence motifs, and their expression demarcates broad chromosomal domains of differential histone methylation and RNA polymerase accessibility. We identified a 2.2 kilobase ncRNA residing in the
HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the
HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of
HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states. |
| doi_str_mv | 10.1016/j.cell.2007.05.022 |
| format | Article |
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HOX loci at five base pair resolution in 11 anatomic sites and identify 231
HOX ncRNAs that extend known transcribed regions by more than 30 kilobases.
HOX ncRNAs are spatially expressed along developmental axes and possess unique sequence motifs, and their expression demarcates broad chromosomal domains of differential histone methylation and RNA polymerase accessibility. We identified a 2.2 kilobase ncRNA residing in the
HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the
HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of
HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2007.05.022</identifier><identifier>PMID: 17604720</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Base Sequence - genetics ; Body Patterning - genetics ; Cells, Cultured ; Chromatin - genetics ; DNA Methylation ; Embryonic Development - genetics ; Epigenesis, Genetic - genetics ; Gene Expression Regulation, Developmental - genetics ; Genes, Homeobox - genetics ; Histones - genetics ; Humans ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Polycomb-Group Proteins ; Regulatory Elements, Transcriptional - genetics ; Repressor Proteins - genetics ; RNA Interference - physiology ; RNA, Untranslated - chemistry ; RNA, Untranslated - genetics</subject><ispartof>Cell, 2007-06, Vol.129 (7), p.1311-1323</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-17f18dd6dfbd3811db77de73d915b31b34b044b0f4c146bed0e94080fd20ca9b3</citedby><cites>FETCH-LOGICAL-c495t-17f18dd6dfbd3811db77de73d915b31b34b044b0f4c146bed0e94080fd20ca9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0092867407006599$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17604720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rinn, John L.</creatorcontrib><creatorcontrib>Kertesz, Michael</creatorcontrib><creatorcontrib>Wang, Jordon K.</creatorcontrib><creatorcontrib>Squazzo, Sharon L.</creatorcontrib><creatorcontrib>Xu, Xiao</creatorcontrib><creatorcontrib>Brugmann, Samantha A.</creatorcontrib><creatorcontrib>Goodnough, L. Henry</creatorcontrib><creatorcontrib>Helms, Jill A.</creatorcontrib><creatorcontrib>Farnham, Peggy J.</creatorcontrib><creatorcontrib>Segal, Eran</creatorcontrib><creatorcontrib>Chang, Howard Y.</creatorcontrib><title>Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs</title><title>Cell</title><addtitle>Cell</addtitle><description>Noncoding RNAs (ncRNA) participate in epigenetic regulation but are poorly understood. Here we characterize the transcriptional landscape of the four human
HOX loci at five base pair resolution in 11 anatomic sites and identify 231
HOX ncRNAs that extend known transcribed regions by more than 30 kilobases.
HOX ncRNAs are spatially expressed along developmental axes and possess unique sequence motifs, and their expression demarcates broad chromosomal domains of differential histone methylation and RNA polymerase accessibility. We identified a 2.2 kilobase ncRNA residing in the
HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the
HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of
HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states.</description><subject>Animals</subject><subject>Base Sequence - genetics</subject><subject>Body Patterning - genetics</subject><subject>Cells, Cultured</subject><subject>Chromatin - genetics</subject><subject>DNA Methylation</subject><subject>Embryonic Development - genetics</subject><subject>Epigenesis, Genetic - genetics</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Genes, Homeobox - genetics</subject><subject>Histones - genetics</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Polycomb-Group Proteins</subject><subject>Regulatory Elements, Transcriptional - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>RNA Interference - physiology</subject><subject>RNA, Untranslated - chemistry</subject><subject>RNA, Untranslated - genetics</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1r3DAQFSWl2Sb5AzkUnXKzO7Jly4Zelt18FJYE2gZyE_oYp1psKbXsQP59ZHaht_agmdHovYd4j5BLBjkDVn_d5wb7Pi8ARA5VDkXxgawYtCLjTBQnZAXQFllTC35KPse4B4CmqqpP5JSJGrgoYEXUzezN5IJXPd3ioEajlhsNHV2n_StS5S396Xr0E938HsOQ3j3dpu58pGm8mweV6sMT3QXjqH6j98GbYJ1_pj_u1_GcfOxUH_Hi2M_I4831r81dtnu4_b5Z7zLD22rKmOhYY21tO23LhjGrhbAoStuySpdMl1wDT6fjhvFaowVsOTTQ2QKManV5Rq4Oui9j-DNjnOTg4mKQ8hjmKAXUFdQN-y-QtaKE9IMELA5AM4YYR-zky-iSRW-SgVwSkHu58OSSgIRKpgQS6ctRfdYD2r-Uo-UJ8O0AwGTGq8NRRuPQG7RuRDNJG9y_9N8BtTSWkA</recordid><startdate>20070629</startdate><enddate>20070629</enddate><creator>Rinn, John L.</creator><creator>Kertesz, Michael</creator><creator>Wang, Jordon K.</creator><creator>Squazzo, Sharon L.</creator><creator>Xu, Xiao</creator><creator>Brugmann, Samantha A.</creator><creator>Goodnough, L. 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HOX loci at five base pair resolution in 11 anatomic sites and identify 231
HOX ncRNAs that extend known transcribed regions by more than 30 kilobases.
HOX ncRNAs are spatially expressed along developmental axes and possess unique sequence motifs, and their expression demarcates broad chromosomal domains of differential histone methylation and RNA polymerase accessibility. We identified a 2.2 kilobase ncRNA residing in the
HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the
HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of
HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17604720</pmid><doi>10.1016/j.cell.2007.05.022</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Base Sequence - genetics Body Patterning - genetics Cells, Cultured Chromatin - genetics DNA Methylation Embryonic Development - genetics Epigenesis, Genetic - genetics Gene Expression Regulation, Developmental - genetics Genes, Homeobox - genetics Histones - genetics Humans Mice Mice, Inbred C57BL Molecular Sequence Data Polycomb-Group Proteins Regulatory Elements, Transcriptional - genetics Repressor Proteins - genetics RNA Interference - physiology RNA, Untranslated - chemistry RNA, Untranslated - genetics |
| title | Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs |
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