Human factor H-related protein 5 (FHR-5). A new complement-associated protein
A novel human plasma protein has been identified as a universal component of complement deposits, when complement is detected immunohistochemically in vivo. The protein is homologous to complement factor H and related proteins and has been designated factor H-related protein 5 (FHR-5). FHR-5 was ide...
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| Veröffentlicht in: | The Journal of biological chemistry 2001-03, Vol.276 (9), p.6747-6754 |
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| container_end_page | 6754 |
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| container_issue | 9 |
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| container_title | The Journal of biological chemistry |
| container_volume | 276 |
| creator | McRae, J L Cowan, P J Power, D A Mitchelhill, K I Kemp, B E Morgan, B P Murphy, B F |
| description | A novel human plasma protein has been identified as a universal component of complement deposits, when complement is detected immunohistochemically in vivo. The protein is homologous to complement factor H and related proteins and has been designated factor H-related protein 5 (FHR-5). FHR-5 was identified by a monoclonal antibody raised using pathologic human glomerular preparations as the immunogen. FHR-5 was purified by affinity chromatography from complement-lysed erythrocytes, and the peptide sequence was obtained. The cDNA was cloned from a human liver library, and FHR-5 was deduced to be a protein containing 551 amino acids organized into nine short consensus repeat motifs. The short consensus repeats of FHR-5 show homology to Factor H and to other Factor H-related proteins, with some unique features demonstrated. Recombinant FHR-5, expressed in insect cells, was shown to bind C3b in vitro. The strong association of FHR-5 with tissue complement deposits in vivo suggests that this additional member of the Factor H family of proteins has a function in complement regulation. |
| doi_str_mv | 10.1074/jbc.M007495200 |
| format | Article |
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The cDNA was cloned from a human liver library, and FHR-5 was deduced to be a protein containing 551 amino acids organized into nine short consensus repeat motifs. The short consensus repeats of FHR-5 show homology to Factor H and to other Factor H-related proteins, with some unique features demonstrated. Recombinant FHR-5, expressed in insect cells, was shown to bind C3b in vitro. 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A new complement-associated protein</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>A novel human plasma protein has been identified as a universal component of complement deposits, when complement is detected immunohistochemically in vivo. The protein is homologous to complement factor H and related proteins and has been designated factor H-related protein 5 (FHR-5). FHR-5 was identified by a monoclonal antibody raised using pathologic human glomerular preparations as the immunogen. FHR-5 was purified by affinity chromatography from complement-lysed erythrocytes, and the peptide sequence was obtained. The cDNA was cloned from a human liver library, and FHR-5 was deduced to be a protein containing 551 amino acids organized into nine short consensus repeat motifs. The short consensus repeats of FHR-5 show homology to Factor H and to other Factor H-related proteins, with some unique features demonstrated. Recombinant FHR-5, expressed in insect cells, was shown to bind C3b in vitro. The strong association of FHR-5 with tissue complement deposits in vivo suggests that this additional member of the Factor H family of proteins has a function in complement regulation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Apolipoproteins - analysis</subject><subject>Blood Proteins - analysis</subject><subject>Blood Proteins - chemistry</subject><subject>Blood Proteins - genetics</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>complement factor H</subject><subject>Complement Factor H - metabolism</subject><subject>complement-associated proteins</subject><subject>FHR-5 protein</subject><subject>Molecular Sequence Data</subject><subject>Rabbits</subject><subject>Recombinant Proteins - analysis</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1Lw0AQxfeg2Fq9epQ9iR4SZ3ezm82xlNYILYLoOezHBFLyZTZB_O8NWMGbc5k5_N68xyPkhkHMIE0ej9bFB5ivTHKAM7IE4CzKuNQLchnCEeZJMnZBFoyB1DLjS3LIp8a0tDRu7AaaRwPWZkRP-6EbsWqppPe7_DWSDzFd0xY_qeuavsYG2zEyIXSu-otfkfPS1AGvT3tF3nfbt00e7V-enjfrfdRzoWehSZAJhw55yjEFz-fInktUiUPrQGRCaAO29Ex4qxKluE299aIsQShTihW5-_k7-35MGMaiqYLDujYtdlMoUlASZJr8CzINoLVWM3h7AifboC_6oWrM8FX8NiW-AQSwZ7Q</recordid><startdate>20010302</startdate><enddate>20010302</enddate><creator>McRae, J L</creator><creator>Cowan, P J</creator><creator>Power, D A</creator><creator>Mitchelhill, K I</creator><creator>Kemp, B E</creator><creator>Morgan, B P</creator><creator>Murphy, B F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010302</creationdate><title>Human factor H-related protein 5 (FHR-5). 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The short consensus repeats of FHR-5 show homology to Factor H and to other Factor H-related proteins, with some unique features demonstrated. Recombinant FHR-5, expressed in insect cells, was shown to bind C3b in vitro. The strong association of FHR-5 with tissue complement deposits in vivo suggests that this additional member of the Factor H family of proteins has a function in complement regulation.</abstract><cop>United States</cop><pmid>11058592</pmid><doi>10.1074/jbc.M007495200</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Apolipoproteins - analysis Blood Proteins - analysis Blood Proteins - chemistry Blood Proteins - genetics Blotting, Northern Blotting, Western Cloning, Molecular complement factor H Complement Factor H - metabolism complement-associated proteins FHR-5 protein Molecular Sequence Data Rabbits Recombinant Proteins - analysis |
| title | Human factor H-related protein 5 (FHR-5). A new complement-associated protein |
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