Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes
Keratinocyte gene expression was surveyed more comprehensively than before, by means of serial analysis of gene expression. A total of 25,694 tags derived from expressed mRNA, were analyzed in a model for normal differentiation and in a model where cultured keratinocytes were stimulated for a prolon...
Gespeichert in:
| Veröffentlicht in: | Journal of investigative dermatology 2001-01, Vol.116 (1), p.12-22 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 22 |
|---|---|
| container_issue | 1 |
| container_start_page | 12 |
| container_title | Journal of investigative dermatology |
| container_volume | 116 |
| creator | Jansen, Bastiaan J.H. van Ruissen, Fred de Jongh, Gys Zeeuwen, Patrick L.J.M. Schalkwijk, Joost |
| description | Keratinocyte gene expression was surveyed more comprehensively than before, by means of serial analysis of gene expression. A total of 25,694 tags derived from expressed mRNA, were analyzed in a model for normal differentiation and in a model where cultured keratinocytes were stimulated for a prolonged period of time with tumor necrosis factor-α, thus mimicking aberrant differentiation in the context of cutaneous inflammation. Serial analysis of gene expression revealed many transcripts derived from unknown genes and a large number of genes that are not known to be expressed in keratinocytes; furthermore, these data provide quantitative information about the relative abundance of transcripts, allowing the identification of differentially expressed genes. A major part of the identified transcripts accounted for genes involved in energy metabolism and protein synthesis. A large proportion of all transcripts (6%) corresponded to genes associated with terminal differentiation and barrier formation. Another highly expressed functional group of genes (2% of all transcripts) corresponded to proteins involved in host protection such as antimicrobial proteins and proteinase inhibitors. Three of these genes were not known to be expressed in keratinocytes, and some were upregulated after prolonged tumor necrosis factor-α exposure. Our data on expressed genes in keratinocytes are consistent with the known function of human epidermis, and provide a first step to generate a transcriptome of human keratinocytes. |
| doi_str_mv | 10.1046/j.1523-1747.2001.00218.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70646105</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022202X1541111X</els_id><sourcerecordid>1038735811</sourcerecordid><originalsourceid>FETCH-LOGICAL-c497t-32dfad1a64de4dc02dfcb86124a2273bb21be31dd2b369de143f4051c617c9ad3</originalsourceid><addsrcrecordid>eNqFkVGL1DAUhYMo7rj6E5Si4Fu7uWmatI-747i7uLAPKvhkSJNbyNCmY9LKzL83dYYRfNmnkJzvHm7OISQDWgDl4mpbQMXKHCSXBaMUCkoZ1MX-GVmdhedklV5Zzij7cUFexbhNoOBV_ZJcAICoZVOuyM-vGJzus2uv-0N0MRu77BY9Zpv9LmCMbvSZ89kn13UY0E9OT2iz9dxPc5IX-m4etM82O2cxDMnpCwY9OT-aw4TxNXnR6T7im9N5Sb5_3nxb3-UPj7f36-uH3PBGTnnJbKctaMEtcmtoupq2FsC4ZkyWbcugxRKsZW0pGovAy47TCowAaRpty0vy8ei7C-OvGeOkBhcN9r32OM5RSSq4AFol8P1_4HacQ_p8VAxoyWsOIkH1ETJhjDFgp3bBDTocFFC1FKC2aslZLTmrpQD1twC1T6PvTv5zO6D9N3hKPAEfToCORvdd0N64eOaaRki5bPD2SHm9BH2WK0Fpw2nSb446plB_OwwqGofeoHUBzaTs6J7e9Q8VMq3b</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>210348416</pqid></control><display><type>article</type><title>Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>ProQuest Central UK/Ireland</source><source>Alma/SFX Local Collection</source><creator>Jansen, Bastiaan J.H. ; van Ruissen, Fred ; de Jongh, Gys ; Zeeuwen, Patrick L.J.M. ; Schalkwijk, Joost</creator><creatorcontrib>Jansen, Bastiaan J.H. ; van Ruissen, Fred ; de Jongh, Gys ; Zeeuwen, Patrick L.J.M. ; Schalkwijk, Joost</creatorcontrib><description>Keratinocyte gene expression was surveyed more comprehensively than before, by means of serial analysis of gene expression. A total of 25,694 tags derived from expressed mRNA, were analyzed in a model for normal differentiation and in a model where cultured keratinocytes were stimulated for a prolonged period of time with tumor necrosis factor-α, thus mimicking aberrant differentiation in the context of cutaneous inflammation. Serial analysis of gene expression revealed many transcripts derived from unknown genes and a large number of genes that are not known to be expressed in keratinocytes; furthermore, these data provide quantitative information about the relative abundance of transcripts, allowing the identification of differentially expressed genes. A major part of the identified transcripts accounted for genes involved in energy metabolism and protein synthesis. A large proportion of all transcripts (6%) corresponded to genes associated with terminal differentiation and barrier formation. Another highly expressed functional group of genes (2% of all transcripts) corresponded to proteins involved in host protection such as antimicrobial proteins and proteinase inhibitors. Three of these genes were not known to be expressed in keratinocytes, and some were upregulated after prolonged tumor necrosis factor-α exposure. Our data on expressed genes in keratinocytes are consistent with the known function of human epidermis, and provide a first step to generate a transcriptome of human keratinocytes.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1046/j.1523-1747.2001.00218.x</identifier><identifier>PMID: 11168793</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>Apoptosis - genetics ; Biological and medical sciences ; Blotting, Northern ; cell culture ; Cell Differentiation - genetics ; Cell Survival - genetics ; Cells, Cultured - drug effects ; Cells, Cultured - physiology ; cytokines ; Cytoskeletal Proteins - genetics ; Dermatology ; differentiation ; epidermis ; Epidermis - cytology ; Gene Expression - drug effects ; genetics ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; RNA, Messenger - genetics ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Journal of investigative dermatology, 2001-01, Vol.116 (1), p.12-22</ispartof><rights>2001 The Society for Investigative Dermatology, Inc</rights><rights>2001 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jan 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-32dfad1a64de4dc02dfcb86124a2273bb21be31dd2b369de143f4051c617c9ad3</citedby><cites>FETCH-LOGICAL-c497t-32dfad1a64de4dc02dfcb86124a2273bb21be31dd2b369de143f4051c617c9ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/210348416?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=996776$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11168793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jansen, Bastiaan J.H.</creatorcontrib><creatorcontrib>van Ruissen, Fred</creatorcontrib><creatorcontrib>de Jongh, Gys</creatorcontrib><creatorcontrib>Zeeuwen, Patrick L.J.M.</creatorcontrib><creatorcontrib>Schalkwijk, Joost</creatorcontrib><title>Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Keratinocyte gene expression was surveyed more comprehensively than before, by means of serial analysis of gene expression. A total of 25,694 tags derived from expressed mRNA, were analyzed in a model for normal differentiation and in a model where cultured keratinocytes were stimulated for a prolonged period of time with tumor necrosis factor-α, thus mimicking aberrant differentiation in the context of cutaneous inflammation. Serial analysis of gene expression revealed many transcripts derived from unknown genes and a large number of genes that are not known to be expressed in keratinocytes; furthermore, these data provide quantitative information about the relative abundance of transcripts, allowing the identification of differentially expressed genes. A major part of the identified transcripts accounted for genes involved in energy metabolism and protein synthesis. A large proportion of all transcripts (6%) corresponded to genes associated with terminal differentiation and barrier formation. Another highly expressed functional group of genes (2% of all transcripts) corresponded to proteins involved in host protection such as antimicrobial proteins and proteinase inhibitors. Three of these genes were not known to be expressed in keratinocytes, and some were upregulated after prolonged tumor necrosis factor-α exposure. Our data on expressed genes in keratinocytes are consistent with the known function of human epidermis, and provide a first step to generate a transcriptome of human keratinocytes.</description><subject>Apoptosis - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>cell culture</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Survival - genetics</subject><subject>Cells, Cultured - drug effects</subject><subject>Cells, Cultured - physiology</subject><subject>cytokines</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Dermatology</subject><subject>differentiation</subject><subject>epidermis</subject><subject>Epidermis - cytology</subject><subject>Gene Expression - drug effects</subject><subject>genetics</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkVGL1DAUhYMo7rj6E5Si4Fu7uWmatI-747i7uLAPKvhkSJNbyNCmY9LKzL83dYYRfNmnkJzvHm7OISQDWgDl4mpbQMXKHCSXBaMUCkoZ1MX-GVmdhedklV5Zzij7cUFexbhNoOBV_ZJcAICoZVOuyM-vGJzus2uv-0N0MRu77BY9Zpv9LmCMbvSZ89kn13UY0E9OT2iz9dxPc5IX-m4etM82O2cxDMnpCwY9OT-aw4TxNXnR6T7im9N5Sb5_3nxb3-UPj7f36-uH3PBGTnnJbKctaMEtcmtoupq2FsC4ZkyWbcugxRKsZW0pGovAy47TCowAaRpty0vy8ei7C-OvGeOkBhcN9r32OM5RSSq4AFol8P1_4HacQ_p8VAxoyWsOIkH1ETJhjDFgp3bBDTocFFC1FKC2aslZLTmrpQD1twC1T6PvTv5zO6D9N3hKPAEfToCORvdd0N64eOaaRki5bPD2SHm9BH2WK0Fpw2nSb446plB_OwwqGofeoHUBzaTs6J7e9Q8VMq3b</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Jansen, Bastiaan J.H.</creator><creator>van Ruissen, Fred</creator><creator>de Jongh, Gys</creator><creator>Zeeuwen, Patrick L.J.M.</creator><creator>Schalkwijk, Joost</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes</title><author>Jansen, Bastiaan J.H. ; van Ruissen, Fred ; de Jongh, Gys ; Zeeuwen, Patrick L.J.M. ; Schalkwijk, Joost</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-32dfad1a64de4dc02dfcb86124a2273bb21be31dd2b369de143f4051c617c9ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Apoptosis - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>cell culture</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Survival - genetics</topic><topic>Cells, Cultured - drug effects</topic><topic>Cells, Cultured - physiology</topic><topic>cytokines</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Dermatology</topic><topic>differentiation</topic><topic>epidermis</topic><topic>Epidermis - cytology</topic><topic>Gene Expression - drug effects</topic><topic>genetics</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jansen, Bastiaan J.H.</creatorcontrib><creatorcontrib>van Ruissen, Fred</creatorcontrib><creatorcontrib>de Jongh, Gys</creatorcontrib><creatorcontrib>Zeeuwen, Patrick L.J.M.</creatorcontrib><creatorcontrib>Schalkwijk, Joost</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jansen, Bastiaan J.H.</au><au>van Ruissen, Fred</au><au>de Jongh, Gys</au><au>Zeeuwen, Patrick L.J.M.</au><au>Schalkwijk, Joost</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>2001-01</date><risdate>2001</risdate><volume>116</volume><issue>1</issue><spage>12</spage><epage>22</epage><pages>12-22</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Keratinocyte gene expression was surveyed more comprehensively than before, by means of serial analysis of gene expression. A total of 25,694 tags derived from expressed mRNA, were analyzed in a model for normal differentiation and in a model where cultured keratinocytes were stimulated for a prolonged period of time with tumor necrosis factor-α, thus mimicking aberrant differentiation in the context of cutaneous inflammation. Serial analysis of gene expression revealed many transcripts derived from unknown genes and a large number of genes that are not known to be expressed in keratinocytes; furthermore, these data provide quantitative information about the relative abundance of transcripts, allowing the identification of differentially expressed genes. A major part of the identified transcripts accounted for genes involved in energy metabolism and protein synthesis. A large proportion of all transcripts (6%) corresponded to genes associated with terminal differentiation and barrier formation. Another highly expressed functional group of genes (2% of all transcripts) corresponded to proteins involved in host protection such as antimicrobial proteins and proteinase inhibitors. Three of these genes were not known to be expressed in keratinocytes, and some were upregulated after prolonged tumor necrosis factor-α exposure. Our data on expressed genes in keratinocytes are consistent with the known function of human epidermis, and provide a first step to generate a transcriptome of human keratinocytes.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>11168793</pmid><doi>10.1046/j.1523-1747.2001.00218.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-202X |
| ispartof | Journal of investigative dermatology, 2001-01, Vol.116 (1), p.12-22 |
| issn | 0022-202X 1523-1747 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70646105 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | Apoptosis - genetics Biological and medical sciences Blotting, Northern cell culture Cell Differentiation - genetics Cell Survival - genetics Cells, Cultured - drug effects Cells, Cultured - physiology cytokines Cytoskeletal Proteins - genetics Dermatology differentiation epidermis Epidermis - cytology Gene Expression - drug effects genetics Humans Investigative techniques, diagnostic techniques (general aspects) Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques RNA, Messenger - genetics Tumor Necrosis Factor-alpha - pharmacology |
| title | Serial Analysis of Gene Expression in Differentiated Cultures of Human Epidermal Keratinocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T18%3A26%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serial%20Analysis%20of%20Gene%20Expression%20in%20Differentiated%20Cultures%20of%20Human%20Epidermal%20Keratinocytes&rft.jtitle=Journal%20of%20investigative%20dermatology&rft.au=Jansen,%20Bastiaan%20J.H.&rft.date=2001-01&rft.volume=116&rft.issue=1&rft.spage=12&rft.epage=22&rft.pages=12-22&rft.issn=0022-202X&rft.eissn=1523-1747&rft.coden=JIDEAE&rft_id=info:doi/10.1046/j.1523-1747.2001.00218.x&rft_dat=%3Cproquest_cross%3E1038735811%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=210348416&rft_id=info:pmid/11168793&rft_els_id=S0022202X1541111X&rfr_iscdi=true |