Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments
A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharos...
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| Veröffentlicht in: | Journal of microbiological methods 2001-02, Vol.44 (1), p.49-58 |
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| creator | Miller, Daniel N. |
| description | A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtration resins, and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns. A method for purifying large solution volumes of DNA extract economically was also developed using low-cost disposable Disposaflex columns. Crude DNA extracts of cattle feedlot soil and aquifer sediment impacted by animal and human wastes were easily purified using the Disposaflex column method regardless of whether a gentle chemical lysis or a bead mill homogenization DNA extraction method was employed. |
| doi_str_mv | 10.1016/S0167-7012(00)00228-1 |
| format | Article |
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Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtration resins, and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns. A method for purifying large solution volumes of DNA extract economically was also developed using low-cost disposable Disposaflex columns. Crude DNA extracts of cattle feedlot soil and aquifer sediment impacted by animal and human wastes were easily purified using the Disposaflex column method regardless of whether a gentle chemical lysis or a bead mill homogenization DNA extraction method was employed.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/S0167-7012(00)00228-1</identifier><identifier>PMID: 11166099</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Agronomy. 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Psychology ; Gels ; Geologic Sediments - microbiology ; Humic Substances - isolation & purification ; Methods and techniques ; Microbial ecology ; PCR ; Polymerase Chain Reaction - methods ; Purification ; Resins, Plant - analysis ; Sediment ; Size exclusion chromatography ; Soil ; Soil Microbiology ; Soil science</subject><ispartof>Journal of microbiological methods, 2001-02, Vol.44 (1), p.49-58</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-8ac500f12f9f5e55719ea93c10f11c8ab80c38faffdae6f9b28842dd4ebe93103</citedby><cites>FETCH-LOGICAL-c535t-8ac500f12f9f5e55719ea93c10f11c8ab80c38faffdae6f9b28842dd4ebe93103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0167-7012(00)00228-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14225945$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11166099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, Daniel N.</creatorcontrib><title>Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtration resins, and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns. A method for purifying large solution volumes of DNA extract economically was also developed using low-cost disposable Disposaflex columns. Crude DNA extracts of cattle feedlot soil and aquifer sediment impacted by animal and human wastes were easily purified using the Disposaflex column method regardless of whether a gentle chemical lysis or a bead mill homogenization DNA extraction method was employed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Chromatography, Gel - methods</subject><subject>Dextrans - analysis</subject><subject>DNA Extraction</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Geologic Sediments - microbiology</subject><subject>Humic Substances - isolation & purification</subject><subject>Methods and techniques</subject><subject>Microbial ecology</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Purification</subject><subject>Resins, Plant - analysis</subject><subject>Sediment</subject><subject>Size exclusion chromatography</subject><subject>Soil</subject><subject>Soil Microbiology</subject><subject>Soil science</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgehS-AmgXKjgkOJx4sQ-IbRqC1IlKj7OluOMqVESF49Tqf--3u6KHnux5dEztvUOY2-BnwKH7tPPsvR1z0F84Pwj50KoGp6xDahe1KqR-jnb_CdH7BXRX85BNq16yY4AoOu41hsWzm7ttNoc4lJFX_3BqfJhymlfSUhhocrHVOVrLMc5Fr6DV9sfdViuwxByTHcVrQNluzgsOMW5ohgmquwyVoRjmHHJ9Jq98HYifHPYj9nv87Nf26_15feLb9svl7WTjcy1sk5y7kF47SVK2YNGqxsHpQZO2UFx1yhvvR8tdl4PQqlWjGOLA-oGeHPMTvb33qT4b0XKZg7kcJrsgnEl0_OubbQST0Lo-14ovYNyD12KRAm9uUlhtunOADe7YZiHYZhd0oZz8zAMA6Xv3eGBdZhxfOw6pF_A-wOw5OzkU0kw0KNrhZC6lcV93jssud0GTIZcwJL2GBK6bMYYnvjKPf2Xp5A</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Miller, Daniel N.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments</title><author>Miller, Daniel N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-8ac500f12f9f5e55719ea93c10f11c8ab80c38faffdae6f9b28842dd4ebe93103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Chromatography, Gel - methods</topic><topic>Dextrans - analysis</topic><topic>DNA Extraction</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Geologic Sediments - microbiology</topic><topic>Humic Substances - isolation & purification</topic><topic>Methods and techniques</topic><topic>Microbial ecology</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Purification</topic><topic>Resins, Plant - analysis</topic><topic>Sediment</topic><topic>Size exclusion chromatography</topic><topic>Soil</topic><topic>Soil Microbiology</topic><topic>Soil science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Daniel N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Daniel N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>44</volume><issue>1</issue><spage>49</spage><epage>58</epage><pages>49-58</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtration resins, and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns. A method for purifying large solution volumes of DNA extract economically was also developed using low-cost disposable Disposaflex columns. Crude DNA extracts of cattle feedlot soil and aquifer sediment impacted by animal and human wastes were easily purified using the Disposaflex column method regardless of whether a gentle chemical lysis or a bead mill homogenization DNA extraction method was employed.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>11166099</pmid><doi>10.1016/S0167-7012(00)00228-1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of microbiological methods, 2001-02, Vol.44 (1), p.49-58 |
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| language | eng |
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| subjects | Agronomy. Soil science and plant productions Animal, plant and microbial ecology Animals Biological and medical sciences Cattle Chromatography, Gel - methods Dextrans - analysis DNA Extraction DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Gels Geologic Sediments - microbiology Humic Substances - isolation & purification Methods and techniques Microbial ecology PCR Polymerase Chain Reaction - methods Purification Resins, Plant - analysis Sediment Size exclusion chromatography Soil Soil Microbiology Soil science |
| title | Evaluation of gel filtration resins for the removal of PCR-inhibitory substances from soils and sediments |
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