Osmotic responses and tolerance limits to changes in external osmolalities, and oolemma permeability characteristics, of human in vitro matured MII oocytes

BACKGROUND Oocyte cryopreservation remains a realistic objective, provided that more systematic approaches are applied, such as thorough analysis of the oocyte oolemma permeability to water and diverse cryoprotectants. METHODS We prospectively investigated volume changes over time at different tempe...

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Veröffentlicht in:	Human reproduction (Oxford) 2007-07, Vol.22 (7), p.1959-1972
Hauptverfasser:	Van den Abbeel, Etienne, Schneider, Ulrich, Liu, Jun, Agca, Yuksel, Critser, John K, Van Steirteghem, André
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Membrane - metabolism Cryopreservation - methods Embryo Culture Techniques Ethylene Glycol Gynecology. Andrology. Obstetrics Humans Medical sciences Metaphase Oocytes - metabolism Osmolar Concentration Osmosis Perfusion Permeability Prospective Studies Specimen Handling Temperature Time Factors
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