CHARACTERIZATION OF OTOSTRONGYLUS CIRCUMLITUS FROM PACIFIC HARBOR AND NORTHERN ELEPHANT SEALS
Otostrongylus circumlitus (Railliet, 1899) from Pacific harbor seals (Phoca vitulina richardsi) and northern elephant seals (Mirounga angustirostris) were examined using morphological and molecular methods to determine whether northern elephant seals along the central California coast are infected b...
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| Veröffentlicht in: | The Journal of parasitology 2001-02, Vol.87 (1), p.73-78 |
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| creator | Elson-Riggins, Jocelyn G Al-Banna, Luma Platzer, Edward G Kaloshian, Isgouhi |
| description | Otostrongylus circumlitus (Railliet, 1899) from Pacific harbor seals (Phoca vitulina richardsi) and northern elephant seals (Mirounga angustirostris) were examined using morphological and molecular methods to determine whether northern elephant seals along the central California coast are infected by the same species of Otostrongylus as are Pacific harbor seals in the same area. Fixed nematodes were examined and measured using light microscopy. The polymerase chain reaction (PCR) was used to amplify and sequence the second internal transcribed spacer (ITS-2) and D3 expansion (26S) regions of ribosomal DNA of O. circumlitus from Pacific harbor and northern elephant seals. The ITS-2 region was also amplified from Parafilaroides sp. from the Pacific harbor seal, northern elephant seal, and California sea lion (Zalophus californianus) and used for restriction fragment length polymorphism (RFLP) analysis. Morphologically, it was not possible to distinguish O. circumlitus from Pacific harbor and northern elephant seals, and over a consensus length of 443 base pairs (bp) for ITS-2 and 321 bp for D3 the sequences of O. circumlitus from both hosts were identical. With the PCR-RFLP assay, it was possible to distinguish O. circumlitus from Parafilaroides sp. The results suggest that O. circumlitus is the same species in Pacific harbor and northern elephant seals, and molecular methods make it possible to distinguish this nematode from related nematodes. |
| doi_str_mv | 10.1645/0022-3395(2001)087[0073:COOCFP]2.0.CO;2 |
| format | Article |
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Fixed nematodes were examined and measured using light microscopy. The polymerase chain reaction (PCR) was used to amplify and sequence the second internal transcribed spacer (ITS-2) and D3 expansion (26S) regions of ribosomal DNA of O. circumlitus from Pacific harbor and northern elephant seals. The ITS-2 region was also amplified from Parafilaroides sp. from the Pacific harbor seal, northern elephant seal, and California sea lion (Zalophus californianus) and used for restriction fragment length polymorphism (RFLP) analysis. Morphologically, it was not possible to distinguish O. circumlitus from Pacific harbor and northern elephant seals, and over a consensus length of 443 base pairs (bp) for ITS-2 and 321 bp for D3 the sequences of O. circumlitus from both hosts were identical. With the PCR-RFLP assay, it was possible to distinguish O. circumlitus from Parafilaroides sp. The results suggest that O. circumlitus is the same species in Pacific harbor and northern elephant seals, and molecular methods make it possible to distinguish this nematode from related nematodes.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/0022-3395(2001)087[0073:COOCFP]2.0.CO;2</identifier><identifier>PMID: 11227905</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Biological and medical sciences ; DNA, Helminth - analysis ; DNA, Helminth - genetics ; DNA, Ribosomal - analysis ; DNA, Ribosomal - genetics ; DNA, Ribosomal Spacer - genetics ; Elephants ; Female ; Fundamental and applied biological sciences. Psychology ; GENETICS-EVOLUTION ; Invertebrates ; Male ; Metastrongyloidea - anatomy & histology ; Metastrongyloidea - classification ; Metastrongyloidea - genetics ; Nemathelminthia. Plathelmintha ; Nematode larvae ; Nematodes ; Nerves ; Parasite hosts ; Parasitology ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Ribosomal DNA ; RNA, Ribosomal - genetics ; Seals ; Seals, Earless - parasitology ; Sequence Analysis, DNA ; Strongylida Infections - parasitology ; Strongylida Infections - veterinary ; Systematics. Geographical distribution ; Vulva</subject><ispartof>The Journal of parasitology, 2001-02, Vol.87 (1), p.73-78</ispartof><rights>American Society of Parasitologists</rights><rights>Copyright 2001 American Society of Parasitologists</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b375t-bf302e681dfc48519ea54c4c91af921b4bda5abf0792a3a19b9426f1516ebb523</citedby><cites>FETCH-LOGICAL-b375t-bf302e681dfc48519ea54c4c91af921b4bda5abf0792a3a19b9426f1516ebb523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1645/0022-3395(2001)087[0073:COOCFP]2.0.CO;2$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3285177$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,26957,27903,27904,52341,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=948387$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11227905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elson-Riggins, Jocelyn G</creatorcontrib><creatorcontrib>Al-Banna, Luma</creatorcontrib><creatorcontrib>Platzer, Edward G</creatorcontrib><creatorcontrib>Kaloshian, Isgouhi</creatorcontrib><title>CHARACTERIZATION OF OTOSTRONGYLUS CIRCUMLITUS FROM PACIFIC HARBOR AND NORTHERN ELEPHANT SEALS</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Otostrongylus circumlitus (Railliet, 1899) from Pacific harbor seals (Phoca vitulina richardsi) and northern elephant seals (Mirounga angustirostris) were examined using morphological and molecular methods to determine whether northern elephant seals along the central California coast are infected by the same species of Otostrongylus as are Pacific harbor seals in the same area. Fixed nematodes were examined and measured using light microscopy. The polymerase chain reaction (PCR) was used to amplify and sequence the second internal transcribed spacer (ITS-2) and D3 expansion (26S) regions of ribosomal DNA of O. circumlitus from Pacific harbor and northern elephant seals. The ITS-2 region was also amplified from Parafilaroides sp. from the Pacific harbor seal, northern elephant seal, and California sea lion (Zalophus californianus) and used for restriction fragment length polymorphism (RFLP) analysis. Morphologically, it was not possible to distinguish O. circumlitus from Pacific harbor and northern elephant seals, and over a consensus length of 443 base pairs (bp) for ITS-2 and 321 bp for D3 the sequences of O. circumlitus from both hosts were identical. With the PCR-RFLP assay, it was possible to distinguish O. circumlitus from Parafilaroides sp. The results suggest that O. circumlitus is the same species in Pacific harbor and northern elephant seals, and molecular methods make it possible to distinguish this nematode from related nematodes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>DNA, Helminth - analysis</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Ribosomal - analysis</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Elephants</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETICS-EVOLUTION</subject><subject>Invertebrates</subject><subject>Male</subject><subject>Metastrongyloidea - anatomy & histology</subject><subject>Metastrongyloidea - classification</subject><subject>Metastrongyloidea - genetics</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Nematode larvae</subject><subject>Nematodes</subject><subject>Nerves</subject><subject>Parasite hosts</subject><subject>Parasitology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Ribosomal DNA</subject><subject>RNA, Ribosomal - genetics</subject><subject>Seals</subject><subject>Seals, Earless - parasitology</subject><subject>Sequence Analysis, DNA</subject><subject>Strongylida Infections - parasitology</subject><subject>Strongylida Infections - veterinary</subject><subject>Systematics. Geographical distribution</subject><subject>Vulva</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqdkE2P0zAQhi0EYsvCX0CWQGg5pOuPOI7hlDVJGykbV2l6ALSy7NSRumqbJW4P_HsSpVo4cxjNHJ6ZefUAcIvRHEchu0WIkIBSwW4IQvgzivlPhDj9IpWS2eqBzNFcqq_kBZhhQXlAaMhegtnz1hV44_0jQogN9RpcYUwIF4jNwINcJlUi67TKfyR1rkqoMqhqta4rVS6-F5s1lHklN_dFXg9zVql7uEpknuUSDpt3qoJJ-Q2WqqqXaVXCtEhXy6Ss4TpNivVb8Ko1e-_eXfo12GRpLZdBoRa5TIrAUs5OgW0pIi6K8bZtwphh4QwLm7AR2LSCYBvarWHGtogLYqjBwoqQRC1mOHLWMkKvwafp7lPf_To7f9KHnW_cfm-Orjt7zVFERRSxAVxMYNN33veu1U_97mD63xojPZrWozM9OtOjaT2Y1qNpPZnWRKNh1OPL95eXZ3tw2793LmoH4MMFML4x-7Y3x2bnnzkRxjTmA_Vxoh79qev_TUMo4pqSQQcfsXTC7K7rju6_U_8BGKCg9w</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Elson-Riggins, Jocelyn G</creator><creator>Al-Banna, Luma</creator><creator>Platzer, Edward G</creator><creator>Kaloshian, Isgouhi</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>CHARACTERIZATION OF OTOSTRONGYLUS CIRCUMLITUS FROM PACIFIC HARBOR AND NORTHERN ELEPHANT SEALS</title><author>Elson-Riggins, Jocelyn G ; Al-Banna, Luma ; Platzer, Edward G ; Kaloshian, Isgouhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b375t-bf302e681dfc48519ea54c4c91af921b4bda5abf0792a3a19b9426f1516ebb523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>DNA, Helminth - analysis</topic><topic>DNA, Helminth - genetics</topic><topic>DNA, Ribosomal - analysis</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Elephants</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETICS-EVOLUTION</topic><topic>Invertebrates</topic><topic>Male</topic><topic>Metastrongyloidea - anatomy & histology</topic><topic>Metastrongyloidea - classification</topic><topic>Metastrongyloidea - genetics</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Nematode larvae</topic><topic>Nematodes</topic><topic>Nerves</topic><topic>Parasite hosts</topic><topic>Parasitology</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Ribosomal DNA</topic><topic>RNA, Ribosomal - genetics</topic><topic>Seals</topic><topic>Seals, Earless - parasitology</topic><topic>Sequence Analysis, DNA</topic><topic>Strongylida Infections - parasitology</topic><topic>Strongylida Infections - veterinary</topic><topic>Systematics. Geographical distribution</topic><topic>Vulva</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elson-Riggins, Jocelyn G</creatorcontrib><creatorcontrib>Al-Banna, Luma</creatorcontrib><creatorcontrib>Platzer, Edward G</creatorcontrib><creatorcontrib>Kaloshian, Isgouhi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elson-Riggins, Jocelyn G</au><au>Al-Banna, Luma</au><au>Platzer, Edward G</au><au>Kaloshian, Isgouhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CHARACTERIZATION OF OTOSTRONGYLUS CIRCUMLITUS FROM PACIFIC HARBOR AND NORTHERN ELEPHANT SEALS</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2001-02</date><risdate>2001</risdate><volume>87</volume><issue>1</issue><spage>73</spage><epage>78</epage><pages>73-78</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Otostrongylus circumlitus (Railliet, 1899) from Pacific harbor seals (Phoca vitulina richardsi) and northern elephant seals (Mirounga angustirostris) were examined using morphological and molecular methods to determine whether northern elephant seals along the central California coast are infected by the same species of Otostrongylus as are Pacific harbor seals in the same area. Fixed nematodes were examined and measured using light microscopy. The polymerase chain reaction (PCR) was used to amplify and sequence the second internal transcribed spacer (ITS-2) and D3 expansion (26S) regions of ribosomal DNA of O. circumlitus from Pacific harbor and northern elephant seals. The ITS-2 region was also amplified from Parafilaroides sp. from the Pacific harbor seal, northern elephant seal, and California sea lion (Zalophus californianus) and used for restriction fragment length polymorphism (RFLP) analysis. Morphologically, it was not possible to distinguish O. circumlitus from Pacific harbor and northern elephant seals, and over a consensus length of 443 base pairs (bp) for ITS-2 and 321 bp for D3 the sequences of O. circumlitus from both hosts were identical. With the PCR-RFLP assay, it was possible to distinguish O. circumlitus from Parafilaroides sp. The results suggest that O. circumlitus is the same species in Pacific harbor and northern elephant seals, and molecular methods make it possible to distinguish this nematode from related nematodes.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>11227905</pmid><doi>10.1645/0022-3395(2001)087[0073:COOCFP]2.0.CO;2</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences DNA, Helminth - analysis DNA, Helminth - genetics DNA, Ribosomal - analysis DNA, Ribosomal - genetics DNA, Ribosomal Spacer - genetics Elephants Female Fundamental and applied biological sciences. Psychology GENETICS-EVOLUTION Invertebrates Male Metastrongyloidea - anatomy & histology Metastrongyloidea - classification Metastrongyloidea - genetics Nemathelminthia. Plathelmintha Nematode larvae Nematodes Nerves Parasite hosts Parasitology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Ribosomal DNA RNA, Ribosomal - genetics Seals Seals, Earless - parasitology Sequence Analysis, DNA Strongylida Infections - parasitology Strongylida Infections - veterinary Systematics. Geographical distribution Vulva |
| title | CHARACTERIZATION OF OTOSTRONGYLUS CIRCUMLITUS FROM PACIFIC HARBOR AND NORTHERN ELEPHANT SEALS |
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