Cloning and biochemical characterization of BglC, a β-glucosidase from the cellulolytic actinomycete Thermobifida fusca

An operon, bglABC, that encodes two sugar permeases and a beta-glucosidase was cloned from a cellulolytic actinomycete, Thermobifida fusca, into Escherichia coli and sequenced. The bglC gene encoding an intracellular beta-glucosidase (beta-d-glucoside glucohydrolase, EC 3.2.1.21) belonging to glycos...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Current microbiology 2001-04, Vol.42 (4), p.295-301
Hauptverfasser:	SPIRIDONOV, Nikolay A, WILSON, David B
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales - enzymology Actinomycetales - genetics Actinomycetales - metabolism Actinomycetes Amino Acid Sequence Bacterial Proteins - analysis Bacteriology Base Sequence beta-Glucosidase BglC protein Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cellobiose - metabolism Cloning, Molecular DNA Primers - chemistry DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification Electrophoresis, Polyacrylamide Gel Escherichia coli - chemistry Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetics Hydrogen-Ion Concentration Microbiology Mission oriented research Molecular Sequence Data Molecular Weight Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Thermobifida fusca
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!