Sequestration of Adenoviral Vector by Kupffer Cells Leads to a Nonlinear Dose Response of Transduction in Liver

Systemic administration of a recombinant adenovirus encoding the human interferon-β gene (H5.110CMVhIFN-β) results in transduction of hepatocytes and detectable circulating levels of IFN-β protein. In preclinical studies in mice, we noticed a distinctly nonlinear dose response, with low levels of vi...

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Veröffentlicht in:	Molecular therapy 2001-01, Vol.3 (1), p.28-35
Hauptverfasser:	Tao, Nianjun, Gao, Guang-Ping, Parr, Michael, Johnston, Julie, Baradet, Timothy, Wilson, James M., Barsoum, James, Fawell, Stephen E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - genetics adenovirus Adenoviruses alpha 1-Antitrypsin - genetics alpha 1-Antitrypsin - metabolism Animals Antibodies beta-Galactosidase - metabolism Carbocyanines - pharmacology Cytomegalovirus dose response Dose-Response Relationship, Drug Endothelium - metabolism Enzyme-Linked Immunosorbent Assay Fluorescent Dyes - pharmacology Gene therapy Genes, Reporter Genetic Vectors Hepatocytes - metabolism Humans Interferon Interferon-beta - genetics Interferon-beta - metabolism interferon-β Kupffer Kupffer Cells - metabolism Labeling Laboratories Liver Liver - metabolism Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Nude Potassium Proteins Species Specificity Tissue Distribution Transduction, Genetic Transgenes - genetics Tumor Cells, Cultured Vectors (Biology) Veins & arteries
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