p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary
Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence in clear cell carcinoma, an infrequent histologic subtype of epithelial ovarian cancer, is rarely reported. The aim of this study is to determine the status of p53 gene alteration in thi...
Gespeichert in:
| Veröffentlicht in: | Gynecologic oncology 2001-02, Vol.80 (2), p.189-193 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 193 |
|---|---|
| container_issue | 2 |
| container_start_page | 189 |
| container_title | Gynecologic oncology |
| container_volume | 80 |
| creator | Shih-Chu Ho, Esther Lai, Chiung-Ru Hsieh, Yeun-Ting Chen, Jung-Ta Lin, Ai-Jane Hung, Man-Jung Liu, Fu-Shing |
| description | Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence in clear cell carcinoma, an infrequent histologic subtype of epithelial ovarian cancer, is rarely reported. The aim of this study is to determine the status of p53 gene alteration in this distinct type of ovarian carcinoma.
Methods. Paraffin blocks of tumors from 38 patients with primary or recurrent ovarian clear cell carcinoma were studied for p53 alteration. All these tumors were subjected to immunohistochemical and molecular analysis. Two monoclonal antibodies (DO-7 and PAb 1801) were used for immunohistochemical staining. Genomic DNAs extracted from paraffin blocks of the 38 tumors were subscribed for a nested polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) analysis. Tumors showing band shift on SSCP were further prepared for DNA sequencing to determine the site of mutation.
Results. Overexpression of p53 was observed in only one stage III clear cell carcinoma. However, focal positive p53 staining was noted in another five tumors. Of the six tumors showing positive immunohistochemistry, p53 alterations were noted in four tumors. Three tumors revealed a missense point mutation: two were in exon 7 (TCT227 → TTT and GGC245 → AGC) and one was in exon 5 (CGC156 → CAC). Another tumor revealed a 12-bp deletion in two possible ways: it might involve the last four codons at the 3′ end of exon 4 (nucleotides 12,288–12,299) or it might cross over the splice junction between exon 4 and intron 4 (nucleotides 12,290–12,301). The former would result in a predicted protein product of 389 amino acids whereas the latter would cause a frameshift in the gene sequence and would result in a truncated protein.
Conclusion. Mutations in p53 appear to be much less frequent in clear cell carcinoma than in other histologic types of epithelial ovarian cancer. We suggest that p53 alterations may not play an important role in the development of clear cell carcinoma. |
| doi_str_mv | 10.1006/gyno.2000.6025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70635227</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0090825800960256</els_id><sourcerecordid>70635227</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-25f5c6745204af12d4433f9551b40e770366f9c75006bb0392da455c1afaa5c3</originalsourceid><addsrcrecordid>eNp1kM9LwzAUgIMobk6vHiUgeGt9SZr-OIkUfwwmu-wesjTRSJvOpBP235uyoidP7_K9x_c-hK4JpAQgv38_uD6lAJDmQPkJmhOoeJKXvDpFc4AKkpLycoYuQviMFANCz9GMEJKTkpdz9LDjDL_tBznY3uFlwEtnvP7aazdg63DdaulxrdsW19Ir6_pO4t7g4UPj9bf0h0t0ZmQb9NU0F2jz_LSpX5PV-mVZP64SlbFsSCg3XOVFxilk0hDaZBljpuKcbDPQRQEsz02lCh5_2m6BVbSRGeeKSCMlV2yB7o5nd76PcmEQnQ0qakmn-30QBeSMU1pEMD2CyvcheG3EztsuigoCYiwmxmJiLCbGYnHhZrq833a6-cOnRBG4nQAZlGyNl07Z8MtVtKQliVR5pHSM8G21F0FZ7ZRurNdqEE1v_zP4AbUXhCY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70635227</pqid></control><display><type>article</type><title>p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Shih-Chu Ho, Esther ; Lai, Chiung-Ru ; Hsieh, Yeun-Ting ; Chen, Jung-Ta ; Lin, Ai-Jane ; Hung, Man-Jung ; Liu, Fu-Shing</creator><creatorcontrib>Shih-Chu Ho, Esther ; Lai, Chiung-Ru ; Hsieh, Yeun-Ting ; Chen, Jung-Ta ; Lin, Ai-Jane ; Hung, Man-Jung ; Liu, Fu-Shing</creatorcontrib><description>Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence in clear cell carcinoma, an infrequent histologic subtype of epithelial ovarian cancer, is rarely reported. The aim of this study is to determine the status of p53 gene alteration in this distinct type of ovarian carcinoma.
Methods. Paraffin blocks of tumors from 38 patients with primary or recurrent ovarian clear cell carcinoma were studied for p53 alteration. All these tumors were subjected to immunohistochemical and molecular analysis. Two monoclonal antibodies (DO-7 and PAb 1801) were used for immunohistochemical staining. Genomic DNAs extracted from paraffin blocks of the 38 tumors were subscribed for a nested polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) analysis. Tumors showing band shift on SSCP were further prepared for DNA sequencing to determine the site of mutation.
Results. Overexpression of p53 was observed in only one stage III clear cell carcinoma. However, focal positive p53 staining was noted in another five tumors. Of the six tumors showing positive immunohistochemistry, p53 alterations were noted in four tumors. Three tumors revealed a missense point mutation: two were in exon 7 (TCT227 → TTT and GGC245 → AGC) and one was in exon 5 (CGC156 → CAC). Another tumor revealed a 12-bp deletion in two possible ways: it might involve the last four codons at the 3′ end of exon 4 (nucleotides 12,288–12,299) or it might cross over the splice junction between exon 4 and intron 4 (nucleotides 12,290–12,301). The former would result in a predicted protein product of 389 amino acids whereas the latter would cause a frameshift in the gene sequence and would result in a truncated protein.
Conclusion. Mutations in p53 appear to be much less frequent in clear cell carcinoma than in other histologic types of epithelial ovarian cancer. We suggest that p53 alterations may not play an important role in the development of clear cell carcinoma.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1006/gyno.2000.6025</identifier><identifier>PMID: 11161858</identifier><identifier>CODEN: GYNOA3</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Adenocarcinoma, Clear Cell - genetics ; Adenocarcinoma, Clear Cell - metabolism ; Adenocarcinoma, Clear Cell - pathology ; Adult ; Aged ; Biological and medical sciences ; clear cell carcinoma ; DNA Mutational Analysis ; Female ; Female genital diseases ; Genes, p53 - genetics ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Medical sciences ; Middle Aged ; Mutation ; Neoplasm Recurrence, Local - genetics ; Neoplasm Recurrence, Local - metabolism ; Neoplasm Recurrence, Local - pathology ; Neoplasm Staging ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; ovary ; p53 ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Tumor Suppressor Protein p53 - biosynthesis ; Tumor Suppressor Protein p53 - genetics ; Tumors</subject><ispartof>Gynecologic oncology, 2001-02, Vol.80 (2), p.189-193</ispartof><rights>2001</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-25f5c6745204af12d4433f9551b40e770366f9c75006bb0392da455c1afaa5c3</citedby><cites>FETCH-LOGICAL-c434t-25f5c6745204af12d4433f9551b40e770366f9c75006bb0392da455c1afaa5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0090825800960256$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=928281$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11161858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shih-Chu Ho, Esther</creatorcontrib><creatorcontrib>Lai, Chiung-Ru</creatorcontrib><creatorcontrib>Hsieh, Yeun-Ting</creatorcontrib><creatorcontrib>Chen, Jung-Ta</creatorcontrib><creatorcontrib>Lin, Ai-Jane</creatorcontrib><creatorcontrib>Hung, Man-Jung</creatorcontrib><creatorcontrib>Liu, Fu-Shing</creatorcontrib><title>p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence in clear cell carcinoma, an infrequent histologic subtype of epithelial ovarian cancer, is rarely reported. The aim of this study is to determine the status of p53 gene alteration in this distinct type of ovarian carcinoma.
Methods. Paraffin blocks of tumors from 38 patients with primary or recurrent ovarian clear cell carcinoma were studied for p53 alteration. All these tumors were subjected to immunohistochemical and molecular analysis. Two monoclonal antibodies (DO-7 and PAb 1801) were used for immunohistochemical staining. Genomic DNAs extracted from paraffin blocks of the 38 tumors were subscribed for a nested polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) analysis. Tumors showing band shift on SSCP were further prepared for DNA sequencing to determine the site of mutation.
Results. Overexpression of p53 was observed in only one stage III clear cell carcinoma. However, focal positive p53 staining was noted in another five tumors. Of the six tumors showing positive immunohistochemistry, p53 alterations were noted in four tumors. Three tumors revealed a missense point mutation: two were in exon 7 (TCT227 → TTT and GGC245 → AGC) and one was in exon 5 (CGC156 → CAC). Another tumor revealed a 12-bp deletion in two possible ways: it might involve the last four codons at the 3′ end of exon 4 (nucleotides 12,288–12,299) or it might cross over the splice junction between exon 4 and intron 4 (nucleotides 12,290–12,301). The former would result in a predicted protein product of 389 amino acids whereas the latter would cause a frameshift in the gene sequence and would result in a truncated protein.
Conclusion. Mutations in p53 appear to be much less frequent in clear cell carcinoma than in other histologic types of epithelial ovarian cancer. We suggest that p53 alterations may not play an important role in the development of clear cell carcinoma.</description><subject>Adenocarcinoma, Clear Cell - genetics</subject><subject>Adenocarcinoma, Clear Cell - metabolism</subject><subject>Adenocarcinoma, Clear Cell - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>clear cell carcinoma</subject><subject>DNA Mutational Analysis</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Genes, p53 - genetics</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Neoplasm Recurrence, Local - genetics</subject><subject>Neoplasm Recurrence, Local - metabolism</subject><subject>Neoplasm Recurrence, Local - pathology</subject><subject>Neoplasm Staging</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>ovary</subject><subject>p53</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Tumor Suppressor Protein p53 - biosynthesis</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumors</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LwzAUgIMobk6vHiUgeGt9SZr-OIkUfwwmu-wesjTRSJvOpBP235uyoidP7_K9x_c-hK4JpAQgv38_uD6lAJDmQPkJmhOoeJKXvDpFc4AKkpLycoYuQviMFANCz9GMEJKTkpdz9LDjDL_tBznY3uFlwEtnvP7aazdg63DdaulxrdsW19Ir6_pO4t7g4UPj9bf0h0t0ZmQb9NU0F2jz_LSpX5PV-mVZP64SlbFsSCg3XOVFxilk0hDaZBljpuKcbDPQRQEsz02lCh5_2m6BVbSRGeeKSCMlV2yB7o5nd76PcmEQnQ0qakmn-30QBeSMU1pEMD2CyvcheG3EztsuigoCYiwmxmJiLCbGYnHhZrq833a6-cOnRBG4nQAZlGyNl07Z8MtVtKQliVR5pHSM8G21F0FZ7ZRurNdqEE1v_zP4AbUXhCY</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Shih-Chu Ho, Esther</creator><creator>Lai, Chiung-Ru</creator><creator>Hsieh, Yeun-Ting</creator><creator>Chen, Jung-Ta</creator><creator>Lin, Ai-Jane</creator><creator>Hung, Man-Jung</creator><creator>Liu, Fu-Shing</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary</title><author>Shih-Chu Ho, Esther ; Lai, Chiung-Ru ; Hsieh, Yeun-Ting ; Chen, Jung-Ta ; Lin, Ai-Jane ; Hung, Man-Jung ; Liu, Fu-Shing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-25f5c6745204af12d4433f9551b40e770366f9c75006bb0392da455c1afaa5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenocarcinoma, Clear Cell - genetics</topic><topic>Adenocarcinoma, Clear Cell - metabolism</topic><topic>Adenocarcinoma, Clear Cell - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>clear cell carcinoma</topic><topic>DNA Mutational Analysis</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Genes, p53 - genetics</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Neoplasm Recurrence, Local - genetics</topic><topic>Neoplasm Recurrence, Local - metabolism</topic><topic>Neoplasm Recurrence, Local - pathology</topic><topic>Neoplasm Staging</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>ovary</topic><topic>p53</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Tumor Suppressor Protein p53 - biosynthesis</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shih-Chu Ho, Esther</creatorcontrib><creatorcontrib>Lai, Chiung-Ru</creatorcontrib><creatorcontrib>Hsieh, Yeun-Ting</creatorcontrib><creatorcontrib>Chen, Jung-Ta</creatorcontrib><creatorcontrib>Lin, Ai-Jane</creatorcontrib><creatorcontrib>Hung, Man-Jung</creatorcontrib><creatorcontrib>Liu, Fu-Shing</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shih-Chu Ho, Esther</au><au>Lai, Chiung-Ru</au><au>Hsieh, Yeun-Ting</au><au>Chen, Jung-Ta</au><au>Lin, Ai-Jane</au><au>Hung, Man-Jung</au><au>Liu, Fu-Shing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>80</volume><issue>2</issue><spage>189</spage><epage>193</epage><pages>189-193</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><coden>GYNOA3</coden><abstract>Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence in clear cell carcinoma, an infrequent histologic subtype of epithelial ovarian cancer, is rarely reported. The aim of this study is to determine the status of p53 gene alteration in this distinct type of ovarian carcinoma.
Methods. Paraffin blocks of tumors from 38 patients with primary or recurrent ovarian clear cell carcinoma were studied for p53 alteration. All these tumors were subjected to immunohistochemical and molecular analysis. Two monoclonal antibodies (DO-7 and PAb 1801) were used for immunohistochemical staining. Genomic DNAs extracted from paraffin blocks of the 38 tumors were subscribed for a nested polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) analysis. Tumors showing band shift on SSCP were further prepared for DNA sequencing to determine the site of mutation.
Results. Overexpression of p53 was observed in only one stage III clear cell carcinoma. However, focal positive p53 staining was noted in another five tumors. Of the six tumors showing positive immunohistochemistry, p53 alterations were noted in four tumors. Three tumors revealed a missense point mutation: two were in exon 7 (TCT227 → TTT and GGC245 → AGC) and one was in exon 5 (CGC156 → CAC). Another tumor revealed a 12-bp deletion in two possible ways: it might involve the last four codons at the 3′ end of exon 4 (nucleotides 12,288–12,299) or it might cross over the splice junction between exon 4 and intron 4 (nucleotides 12,290–12,301). The former would result in a predicted protein product of 389 amino acids whereas the latter would cause a frameshift in the gene sequence and would result in a truncated protein.
Conclusion. Mutations in p53 appear to be much less frequent in clear cell carcinoma than in other histologic types of epithelial ovarian cancer. We suggest that p53 alterations may not play an important role in the development of clear cell carcinoma.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>11161858</pmid><doi>10.1006/gyno.2000.6025</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0090-8258 |
| ispartof | Gynecologic oncology, 2001-02, Vol.80 (2), p.189-193 |
| issn | 0090-8258 1095-6859 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70635227 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenocarcinoma, Clear Cell - genetics Adenocarcinoma, Clear Cell - metabolism Adenocarcinoma, Clear Cell - pathology Adult Aged Biological and medical sciences clear cell carcinoma DNA Mutational Analysis Female Female genital diseases Genes, p53 - genetics Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Medical sciences Middle Aged Mutation Neoplasm Recurrence, Local - genetics Neoplasm Recurrence, Local - metabolism Neoplasm Recurrence, Local - pathology Neoplasm Staging Ovarian Neoplasms - genetics Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology ovary p53 Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Tumor Suppressor Protein p53 - biosynthesis Tumor Suppressor Protein p53 - genetics Tumors |
| title | p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T16%3A40%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=p53%20Mutation%20Is%20Infrequent%20in%20Clear%20Cell%20Carcinoma%20of%20the%20Ovary&rft.jtitle=Gynecologic%20oncology&rft.au=Shih-Chu%20Ho,%20Esther&rft.date=2001-02-01&rft.volume=80&rft.issue=2&rft.spage=189&rft.epage=193&rft.pages=189-193&rft.issn=0090-8258&rft.eissn=1095-6859&rft.coden=GYNOA3&rft_id=info:doi/10.1006/gyno.2000.6025&rft_dat=%3Cproquest_cross%3E70635227%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70635227&rft_id=info:pmid/11161858&rft_els_id=S0090825800960256&rfr_iscdi=true |