Single strand mRNA differential display (SSDD) applied to the identification of serine/threonine phosphatases regulated during cerebellar development

Differential display is a used widely and useful technique for the study of differentially expressed genes. However, very poor results have been obtained in the past when particular gene families were studied. Initially, we attempted to study the mRNA expression of catalytic subunits of serine/threo...

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Veröffentlicht in:	Journal of neuroscience methods 2001-01, Vol.105 (1), p.87-94
Hauptverfasser:	Vilá-Ortiz, Guillermo J., Radrizzzani, Martín, Carminatti, Héctor, Idoyaga-Vargas, Víctor P., Santa-Coloma, Tomás A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alternative Splicing - genetics Animals Biological and medical sciences Blotting, Northern Cell Differentiation - genetics Cerebellum Cerebellum - enzymology Cerebellum - growth & development Development Development. Senescence. Regeneration. Transplantation Differential display Fundamental and applied biological sciences. Psychology Gene Expression Profiling - methods Gene Expression Regulation, Developmental - genetics Mice Mice, Inbred C57BL Nerve Tissue Proteins - metabolism Phosphoprotein Phosphatases - chemistry Phosphoprotein Phosphatases - genetics Phosphorylation Polymorphism, Single-Stranded Conformational RNA, Messenger - analysis Serine threonine phosphatases Single strand conformation polymorphism Single strand differential display Synapses - genetics Synapses - metabolism Vertebrates: nervous system and sense organs
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