STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells
Telomerase is active in immature somatic cells, but not in differentiated cells. However, the regulation during cell differentiation is not well understood. In this study, a human chronic myelogenous leukemia cell line (K562) was induced to differentiate into megakaryocytes by TPA, and erythroid by...
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| Veröffentlicht in: | Cell cycle (Georgetown, Tex.) Tex.), 2007-06, Vol.6 (12), p.1496-1501 |
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| creator | Nakatake, Mayuka Kakiuchi, Yasutaka Sasaki, Narie Murakami-Murofushi, Kimiko Yamada, Osamu |
| description | Telomerase is active in immature somatic cells, but not in differentiated cells. However, the regulation during cell differentiation is not well understood. In this study, a human chronic myelogenous leukemia cell line (K562) was induced to differentiate into megakaryocytes by TPA, and erythroid by STI571. A human acute myeloblastic leukemia cell line (HL60) was also induced to differentiate into monocytes by TPA and VD3, and granulocyte by ATRA. TPA induced transient increase of telomerase activity (mainly nuclear fraction) during megakaryocytic differentiation, while the expression of hTERT decreased gradually throughout the same period. Pretreatment with PKC inhibitors inhibited the megakaryocytic differentiation, transient increase of telomerase activity, while recombinant PKC increased telomerase activity. ChIP assay resulted STAT3 and STAT5 dissociated from the hTERT promoter, indicating that STAT3 and STAT5 are one of the transcriptional regulators. These results suggest that telomerase activity is regulated by two mechanisms during megakaryocytic differentiation. |
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However, the regulation during cell differentiation is not well understood. In this study, a human chronic myelogenous leukemia cell line (K562) was induced to differentiate into megakaryocytes by TPA, and erythroid by STI571. A human acute myeloblastic leukemia cell line (HL60) was also induced to differentiate into monocytes by TPA and VD3, and granulocyte by ATRA. TPA induced transient increase of telomerase activity (mainly nuclear fraction) during megakaryocytic differentiation, while the expression of hTERT decreased gradually throughout the same period. Pretreatment with PKC inhibitors inhibited the megakaryocytic differentiation, transient increase of telomerase activity, while recombinant PKC increased telomerase activity. ChIP assay resulted STAT3 and STAT5 dissociated from the hTERT promoter, indicating that STAT3 and STAT5 are one of the transcriptional regulators. These results suggest that telomerase activity is regulated by two mechanisms during megakaryocytic differentiation.</description><identifier>EISSN: 1551-4005</identifier><identifier>PMID: 17525530</identifier><language>eng</language><publisher>United States</publisher><subject>Acetophenones - pharmacology ; Benzopyrans - pharmacology ; Binding Sites - genetics ; Blotting, Western ; Cell Differentiation - physiology ; Chromatin Immunoprecipitation ; Dimethyl Sulfoxide ; DNA Primers - genetics ; Gene Expression Regulation, Enzymologic - physiology ; HL-60 Cells ; Humans ; K562 Cells ; Megakaryocytes - cytology ; Megakaryocytes - enzymology ; Megakaryocytes - physiology ; Promoter Regions, Genetic - genetics ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; STAT3 Transcription Factor - metabolism ; Telomerase - metabolism ; Transcription Factors - metabolism</subject><ispartof>Cell cycle (Georgetown, Tex.), 2007-06, Vol.6 (12), p.1496-1501</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17525530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakatake, Mayuka</creatorcontrib><creatorcontrib>Kakiuchi, Yasutaka</creatorcontrib><creatorcontrib>Sasaki, Narie</creatorcontrib><creatorcontrib>Murakami-Murofushi, Kimiko</creatorcontrib><creatorcontrib>Yamada, Osamu</creatorcontrib><title>STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells</title><title>Cell cycle (Georgetown, Tex.)</title><addtitle>Cell Cycle</addtitle><description>Telomerase is active in immature somatic cells, but not in differentiated cells. However, the regulation during cell differentiation is not well understood. In this study, a human chronic myelogenous leukemia cell line (K562) was induced to differentiate into megakaryocytes by TPA, and erythroid by STI571. A human acute myeloblastic leukemia cell line (HL60) was also induced to differentiate into monocytes by TPA and VD3, and granulocyte by ATRA. TPA induced transient increase of telomerase activity (mainly nuclear fraction) during megakaryocytic differentiation, while the expression of hTERT decreased gradually throughout the same period. Pretreatment with PKC inhibitors inhibited the megakaryocytic differentiation, transient increase of telomerase activity, while recombinant PKC increased telomerase activity. ChIP assay resulted STAT3 and STAT5 dissociated from the hTERT promoter, indicating that STAT3 and STAT5 are one of the transcriptional regulators. These results suggest that telomerase activity is regulated by two mechanisms during megakaryocytic differentiation.</description><subject>Acetophenones - pharmacology</subject><subject>Benzopyrans - pharmacology</subject><subject>Binding Sites - genetics</subject><subject>Blotting, Western</subject><subject>Cell Differentiation - physiology</subject><subject>Chromatin Immunoprecipitation</subject><subject>Dimethyl Sulfoxide</subject><subject>DNA Primers - genetics</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>K562 Cells</subject><subject>Megakaryocytes - cytology</subject><subject>Megakaryocytes - enzymology</subject><subject>Megakaryocytes - physiology</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Telomerase - metabolism</subject><subject>Transcription Factors - metabolism</subject><issn>1551-4005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLAzEYRYMgtlb_gmTlbiCPSZMsS_FFCwrW9ZBMvpRoZqYmGWH-vRUruLqbw-Hee4bmVAha1YSIGbrM-Z0QpqSmF2hGpWBCcDJH3etutePY9A6_bNbYBe8hQV-CiXHCCfZjNAVwgTh0kEwGbNoSvkKZsBtT6Pe4g735MGka2qmE9r-hhKHHg8cbsWS4hRjzFTr3Jma4PuUCvd3f7daP1fb54Wm92lYHynWpqACpLHOK1K2reS2IZmCddEoZx7Sw1hDinW6t8s5Iyp1QgllYeqa1I5Iv0O2v95CGzxFyabqQfxqYHoYxN5IsOeOcHsGbEzjaDlxzSKE7bmn-DuLfzD1iNQ</recordid><startdate>20070615</startdate><enddate>20070615</enddate><creator>Nakatake, Mayuka</creator><creator>Kakiuchi, Yasutaka</creator><creator>Sasaki, Narie</creator><creator>Murakami-Murofushi, Kimiko</creator><creator>Yamada, Osamu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20070615</creationdate><title>STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells</title><author>Nakatake, Mayuka ; Kakiuchi, Yasutaka ; Sasaki, Narie ; Murakami-Murofushi, Kimiko ; Yamada, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p139t-15e78b2d804cd4345092ebd7d88ad295bba00fd9cb8fda713d5852be6f299d073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetophenones - pharmacology</topic><topic>Benzopyrans - pharmacology</topic><topic>Binding Sites - genetics</topic><topic>Blotting, Western</topic><topic>Cell Differentiation - physiology</topic><topic>Chromatin Immunoprecipitation</topic><topic>Dimethyl Sulfoxide</topic><topic>DNA Primers - genetics</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>K562 Cells</topic><topic>Megakaryocytes - cytology</topic><topic>Megakaryocytes - enzymology</topic><topic>Megakaryocytes - physiology</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Telomerase - metabolism</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakatake, Mayuka</creatorcontrib><creatorcontrib>Kakiuchi, Yasutaka</creatorcontrib><creatorcontrib>Sasaki, Narie</creatorcontrib><creatorcontrib>Murakami-Murofushi, Kimiko</creatorcontrib><creatorcontrib>Yamada, Osamu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cell cycle (Georgetown, Tex.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakatake, Mayuka</au><au>Kakiuchi, Yasutaka</au><au>Sasaki, Narie</au><au>Murakami-Murofushi, Kimiko</au><au>Yamada, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells</atitle><jtitle>Cell cycle (Georgetown, Tex.)</jtitle><addtitle>Cell Cycle</addtitle><date>2007-06-15</date><risdate>2007</risdate><volume>6</volume><issue>12</issue><spage>1496</spage><epage>1501</epage><pages>1496-1501</pages><eissn>1551-4005</eissn><abstract>Telomerase is active in immature somatic cells, but not in differentiated cells. However, the regulation during cell differentiation is not well understood. In this study, a human chronic myelogenous leukemia cell line (K562) was induced to differentiate into megakaryocytes by TPA, and erythroid by STI571. A human acute myeloblastic leukemia cell line (HL60) was also induced to differentiate into monocytes by TPA and VD3, and granulocyte by ATRA. TPA induced transient increase of telomerase activity (mainly nuclear fraction) during megakaryocytic differentiation, while the expression of hTERT decreased gradually throughout the same period. Pretreatment with PKC inhibitors inhibited the megakaryocytic differentiation, transient increase of telomerase activity, while recombinant PKC increased telomerase activity. ChIP assay resulted STAT3 and STAT5 dissociated from the hTERT promoter, indicating that STAT3 and STAT5 are one of the transcriptional regulators. These results suggest that telomerase activity is regulated by two mechanisms during megakaryocytic differentiation.</abstract><cop>United States</cop><pmid>17525530</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Cell cycle (Georgetown, Tex.), 2007-06, Vol.6 (12), p.1496-1501 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Acetophenones - pharmacology Benzopyrans - pharmacology Binding Sites - genetics Blotting, Western Cell Differentiation - physiology Chromatin Immunoprecipitation Dimethyl Sulfoxide DNA Primers - genetics Gene Expression Regulation, Enzymologic - physiology HL-60 Cells Humans K562 Cells Megakaryocytes - cytology Megakaryocytes - enzymology Megakaryocytes - physiology Promoter Regions, Genetic - genetics Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Reverse Transcriptase Polymerase Chain Reaction STAT3 Transcription Factor - metabolism Telomerase - metabolism Transcription Factors - metabolism |
| title | STAT3 and PKC differentially regulate telomerase activity during megakaryocytic differentiation of K562 cells |
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