Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells
Background The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and ma...
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Veröffentlicht in: | The Prostate 2001-01, Vol.46 (1), p.68-75 |
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creator | Aalamian, Maryam Pirtskhalaishvili, Georgi Nunez, Anthony Esche, Clemens Shurin, Galina V. Huland, Edith Huland, Hartwig Shurin, Michael R. |
description | Background
The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro.
Methods
DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation.
Results
Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited.
Conclusions
Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc. |
doi_str_mv | 10.1002/1097-0045(200101)46:1<68::AID-PROS1010>3.0.CO;2-2 |
format | Article |
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The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro.
Methods
DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation.
Results
Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited.
Conclusions
Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/1097-0045(200101)46:1<68::AID-PROS1010>3.0.CO;2-2</identifier><identifier>PMID: 11170134</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Antigens, CD ; Antigens, CD1 - analysis ; Biological and medical sciences ; CD83 ; CD83 Antigen ; dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - pathology ; Flow Cytometry ; Histocytochemistry ; Humans ; Immunoglobulins ; immunosuppression ; LNCaP ; Lymphocyte Culture Test, Mixed ; Male ; Medical sciences ; Membrane Glycoproteins ; Monocytes - immunology ; Monocytes - pathology ; Nephrology. Urinary tract diseases ; prostate cancer ; Prostatic Neoplasms - immunology ; Prostatic Neoplasms - pathology ; Scintillation Counting ; Tritium ; Tumor Cells, Cultured ; Tumors of the urinary system ; Urinary tract. Prostate gland</subject><ispartof>The Prostate, 2001-01, Vol.46 (1), p.68-75</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4260-f6e3bd8a21fd58807a9c0997faf61798e4ceca89ffef780b2ccc6e840aed93083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1097-0045%28200101%2946%3A1%3C68%3A%3AAID-PROS1010%3E3.0.CO%3B2-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1097-0045%28200101%2946%3A1%3C68%3A%3AAID-PROS1010%3E3.0.CO%3B2-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=873627$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11170134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aalamian, Maryam</creatorcontrib><creatorcontrib>Pirtskhalaishvili, Georgi</creatorcontrib><creatorcontrib>Nunez, Anthony</creatorcontrib><creatorcontrib>Esche, Clemens</creatorcontrib><creatorcontrib>Shurin, Galina V.</creatorcontrib><creatorcontrib>Huland, Edith</creatorcontrib><creatorcontrib>Huland, Hartwig</creatorcontrib><creatorcontrib>Shurin, Michael R.</creatorcontrib><title>Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>Background
The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro.
Methods
DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation.
Results
Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited.
Conclusions
Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Antigens, CD</subject><subject>Antigens, CD1 - analysis</subject><subject>Biological and medical sciences</subject><subject>CD83</subject><subject>CD83 Antigen</subject><subject>dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - pathology</subject><subject>Flow Cytometry</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>immunosuppression</subject><subject>LNCaP</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins</subject><subject>Monocytes - immunology</subject><subject>Monocytes - pathology</subject><subject>Nephrology. Urinary tract diseases</subject><subject>prostate cancer</subject><subject>Prostatic Neoplasms - immunology</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Scintillation Counting</subject><subject>Tritium</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFtvEzEQRlcIREPhL6CVkBB92DC2N7Y3oEolQFopIqXcntDI8Y6rLXsp9i6Qf4-jDeEJiSdrRt8cz5wkKRhMGQB_zqBQGUA-e8YBGLCTXM7ZS6nn87OL19nl1fpDbMKpmMJ0sX7BM34nmRxm7iYT4AqynAl1lDwI4WbHiNj7yRFjTAET-ST5ej40pk1vfRd601NqTWvJp56uhzrWIb2mlrzpq65NTVumjemHfdm5tOnazm57ykry1Q8q05La0ld9ZVNLdR0eJvecqQM92r_Hyae3bz4uzrPVenmxOFtlNucSMidJbEptOHPlTGtQprBQFMoZJ5kqNOWWrNGFc-SUhg231krSORgqCwFaHCdPR2684_tAocemCrsNTEvdEFCBFIzNZAy-H4M2Hhw8Obz1VWP8Fhngzjnu_OHOH47OMZfIUGrE6Bz_OEeBgIs1cuSR-Xj_-bBpqPxL3EuOgSf7gAnW1M5Hx1U45LQSkquY-jymflY1bf9_r3-sdehFcDaCq9DTrwPY-G8olVAz_PJuiZpfaVhdvsKl-A3CU7fb</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Aalamian, Maryam</creator><creator>Pirtskhalaishvili, Georgi</creator><creator>Nunez, Anthony</creator><creator>Esche, Clemens</creator><creator>Shurin, Galina V.</creator><creator>Huland, Edith</creator><creator>Huland, Hartwig</creator><creator>Shurin, Michael R.</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells</title><author>Aalamian, Maryam ; Pirtskhalaishvili, Georgi ; Nunez, Anthony ; Esche, Clemens ; Shurin, Galina V. ; Huland, Edith ; Huland, Hartwig ; Shurin, Michael R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4260-f6e3bd8a21fd58807a9c0997faf61798e4ceca89ffef780b2ccc6e840aed93083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Antigens, CD</topic><topic>Antigens, CD1 - analysis</topic><topic>Biological and medical sciences</topic><topic>CD83</topic><topic>CD83 Antigen</topic><topic>dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - pathology</topic><topic>Flow Cytometry</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Immunoglobulins</topic><topic>immunosuppression</topic><topic>LNCaP</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins</topic><topic>Monocytes - immunology</topic><topic>Monocytes - pathology</topic><topic>Nephrology. Urinary tract diseases</topic><topic>prostate cancer</topic><topic>Prostatic Neoplasms - immunology</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Scintillation Counting</topic><topic>Tritium</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aalamian, Maryam</creatorcontrib><creatorcontrib>Pirtskhalaishvili, Georgi</creatorcontrib><creatorcontrib>Nunez, Anthony</creatorcontrib><creatorcontrib>Esche, Clemens</creatorcontrib><creatorcontrib>Shurin, Galina V.</creatorcontrib><creatorcontrib>Huland, Edith</creatorcontrib><creatorcontrib>Huland, Hartwig</creatorcontrib><creatorcontrib>Shurin, Michael R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aalamian, Maryam</au><au>Pirtskhalaishvili, Georgi</au><au>Nunez, Anthony</au><au>Esche, Clemens</au><au>Shurin, Galina V.</au><au>Huland, Edith</au><au>Huland, Hartwig</au><au>Shurin, Michael R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2001-01</date><risdate>2001</risdate><volume>46</volume><issue>1</issue><spage>68</spage><epage>75</epage><pages>68-75</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><coden>PRSTDS</coden><abstract>Background
The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro.
Methods
DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation.
Results
Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited.
Conclusions
Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11170134</pmid><doi>10.1002/1097-0045(200101)46:1<68::AID-PROS1010>3.0.CO;2-2</doi><tpages>8</tpages></addata></record> |
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subjects | Antigens, CD Antigens, CD1 - analysis Biological and medical sciences CD83 CD83 Antigen dendritic cells Dendritic Cells - immunology Dendritic Cells - pathology Flow Cytometry Histocytochemistry Humans Immunoglobulins immunosuppression LNCaP Lymphocyte Culture Test, Mixed Male Medical sciences Membrane Glycoproteins Monocytes - immunology Monocytes - pathology Nephrology. Urinary tract diseases prostate cancer Prostatic Neoplasms - immunology Prostatic Neoplasms - pathology Scintillation Counting Tritium Tumor Cells, Cultured Tumors of the urinary system Urinary tract. Prostate gland |
title | Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells |
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