Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells

Background The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and ma...

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Veröffentlicht in:The Prostate 2001-01, Vol.46 (1), p.68-75
Hauptverfasser: Aalamian, Maryam, Pirtskhalaishvili, Georgi, Nunez, Anthony, Esche, Clemens, Shurin, Galina V., Huland, Edith, Huland, Hartwig, Shurin, Michael R.
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container_end_page 75
container_issue 1
container_start_page 68
container_title The Prostate
container_volume 46
creator Aalamian, Maryam
Pirtskhalaishvili, Georgi
Nunez, Anthony
Esche, Clemens
Shurin, Galina V.
Huland, Edith
Huland, Hartwig
Shurin, Michael R.
description Background The progression of prostate cancer is accompanied by a marked suppression of the immune system, including the apoptotic death of dendritic cells (DC) responsible for the induction of antitumor immunity. In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro. Methods DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation. Results Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited. Conclusions Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.
doi_str_mv 10.1002/1097-0045(200101)46:1<68::AID-PROS1010>3.0.CO;2-2
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In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro. Methods DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation. Results Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited. Conclusions Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/1097-0045(200101)46:1&lt;68::AID-PROS1010&gt;3.0.CO;2-2</identifier><identifier>PMID: 11170134</identifier><identifier>CODEN: PRSTDS</identifier><language>eng</language><publisher>New York: John Wiley &amp; Sons, Inc</publisher><subject>Antigens, CD ; Antigens, CD1 - analysis ; Biological and medical sciences ; CD83 ; CD83 Antigen ; dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - pathology ; Flow Cytometry ; Histocytochemistry ; Humans ; Immunoglobulins ; immunosuppression ; LNCaP ; Lymphocyte Culture Test, Mixed ; Male ; Medical sciences ; Membrane Glycoproteins ; Monocytes - immunology ; Monocytes - pathology ; Nephrology. Urinary tract diseases ; prostate cancer ; Prostatic Neoplasms - immunology ; Prostatic Neoplasms - pathology ; Scintillation Counting ; Tritium ; Tumor Cells, Cultured ; Tumors of the urinary system ; Urinary tract. 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In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro. Methods DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation. Results Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited. Conclusions Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. Prostate 46:68–75, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Antigens, CD</subject><subject>Antigens, CD1 - analysis</subject><subject>Biological and medical sciences</subject><subject>CD83</subject><subject>CD83 Antigen</subject><subject>dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - pathology</subject><subject>Flow Cytometry</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>immunosuppression</subject><subject>LNCaP</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins</subject><subject>Monocytes - immunology</subject><subject>Monocytes - pathology</subject><subject>Nephrology. Urinary tract diseases</subject><subject>prostate cancer</subject><subject>Prostatic Neoplasms - immunology</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Scintillation Counting</subject><subject>Tritium</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. 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Urinary tract diseases</topic><topic>prostate cancer</topic><topic>Prostatic Neoplasms - immunology</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Scintillation Counting</topic><topic>Tritium</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. 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In this study, we evaluated whether prostate cancer might inhibit DC generation and maturation in vitro. Methods DC were generated from peripheral blood monocytes in the presence of the human prostate cell line LNCaP or nonmalignant cells, and characterized by light microscopy, FACScan analysis, and ability to stimulate T‐cell proliferation. Results Prostate cancer significantly inhibited the conversion of monocytes into DC, which was assessed by the expression of DC markers CD1a and CD83. These cells were weak stimulators of T‐cell proliferation, suggesting that DC generated in the prostate cancer microenvironment are functionally inhibited. Conclusions Prostate cancer not only kills mature DC, but also inhibits their generation and maturation, resulting in decreased production of antigen‐presenting cells and inhibition of their functional activity. 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subjects Antigens, CD
Antigens, CD1 - analysis
Biological and medical sciences
CD83
CD83 Antigen
dendritic cells
Dendritic Cells - immunology
Dendritic Cells - pathology
Flow Cytometry
Histocytochemistry
Humans
Immunoglobulins
immunosuppression
LNCaP
Lymphocyte Culture Test, Mixed
Male
Medical sciences
Membrane Glycoproteins
Monocytes - immunology
Monocytes - pathology
Nephrology. Urinary tract diseases
prostate cancer
Prostatic Neoplasms - immunology
Prostatic Neoplasms - pathology
Scintillation Counting
Tritium
Tumor Cells, Cultured
Tumors of the urinary system
Urinary tract. Prostate gland
title Human prostate cancer regulates generation and maturation of monocyte-derived dendritic cells
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