New broad-host-range promoter probe vectors based on the plasmid RK2 replicon

Abstract Broad-host-range plasmid RK2-based promoter probe vectors with a known nucleotide sequence were constructed. In the absence of an upstream promoter, the expression of two tested reporter genes (luc and lacZ) in Escherichia coli was virtually zero, while insertion of the Ptrc promoter result...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEMS microbiology letters 2001-02, Vol.195 (1), p.91-96
Hauptverfasser:	Santos, Pedro Miguel, Di Bartolo, Ilaria, Blatny, Janet Martha, Zennaro, Elisabetta, Valla, Svein
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences Broad‐host‐range Burkholderia Copy number DNA Probes E coli Expression vectors Fundamental and applied biological sciences. Psychology Gene expression Genetic Vectors Genetics Gram-Negative Bacteria - genetics lacZ gene luc gene luciferase Microbiology Molecular biology Molecular Sequence Data Nucleotide sequence Nucleotides Plasmid Plasmids - genetics Promoter probe Promoter Regions, Genetic - genetics Pseudomonas fluorescens Pseudomonas putida Replication Origin - genetics Replicon - genetics RK2 Sphingomonas Vector
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!