CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis
Background: Recent studies have reported that TH1 and TH2 cells express CXCR3 and CCR4, respectively. Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with TH2 and TH1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatiti...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 2001-02, Vol.107 (2), p.353-358 |
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| creator | Nakatani, Tomomi Kaburagi, Yuko Shimada, Yuka Inaoki, Makoto Takehara, Kazuhiko Mukaida, Naofumi Sato, Shinichi |
| description | Background: Recent studies have reported that TH1 and TH2 cells express CXCR3 and CCR4, respectively. Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with TH2 and TH1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatitis (AD). Methods: Intracellular cytokine production and chemokine receptor expression in blood T cells were examined by flow cytometry. Immunohistochemical expression of chemokine receptors was also investigated in chronically lesional skin. Results: CCR4+ and CXCR3+ CD4+ T cells predominantly produced IL-4 and IFN-γ, respectively. Although the frequency of CXCR3+ cells among CD4+ CD45RO+ T cells was similar for patients with AD (n = 29) and healthy control subjects (n = 19), patients with severe AD (n = 14) had a reduced frequency of CXCR3+ cells. In contrast, the frequency of CCR4+ cells and the CCR4/CXCR3 ratio were higher in patients with AD (n = 22) than healthy control subjects (n = 16) and correlated with disease severity of AD. The frequency of CCR4+ cells correlated positively with eosinophil numbers and serum IgE levels, whereas the frequency of CXCR3+ cells correlated inversely with eosinophil numbers. The frequency of CCR4+ or CXCR3+ cells was similar in patients with psoriasis (n = 6) and healthy control subjects. Immunohistochemical analysis showed that the frequency of CCR4+ cells among CD4+ T cells in chronically lesional skin of patients with AD (n = 9) was higher than that of patients with psoriasis (n = 4). Conclusion: Our data suggest the association of CCR4 expression with TH2 cells, the predominance of CCR4+ cells in blood from patients with AD, and an important role of CCR4 in the migration of TH2 cells from blood into AD lesional skin. (J Allergy Clin Immunol 2001;107:353-8.) |
| doi_str_mv | 10.1067/mai.2001.112601 |
| format | Article |
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Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with TH2 and TH1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatitis (AD). Methods: Intracellular cytokine production and chemokine receptor expression in blood T cells were examined by flow cytometry. Immunohistochemical expression of chemokine receptors was also investigated in chronically lesional skin. Results: CCR4+ and CXCR3+ CD4+ T cells predominantly produced IL-4 and IFN-γ, respectively. Although the frequency of CXCR3+ cells among CD4+ CD45RO+ T cells was similar for patients with AD (n = 29) and healthy control subjects (n = 19), patients with severe AD (n = 14) had a reduced frequency of CXCR3+ cells. In contrast, the frequency of CCR4+ cells and the CCR4/CXCR3 ratio were higher in patients with AD (n = 22) than healthy control subjects (n = 16) and correlated with disease severity of AD. The frequency of CCR4+ cells correlated positively with eosinophil numbers and serum IgE levels, whereas the frequency of CXCR3+ cells correlated inversely with eosinophil numbers. The frequency of CCR4+ or CXCR3+ cells was similar in patients with psoriasis (n = 6) and healthy control subjects. Immunohistochemical analysis showed that the frequency of CCR4+ cells among CD4+ T cells in chronically lesional skin of patients with AD (n = 9) was higher than that of patients with psoriasis (n = 4). Conclusion: Our data suggest the association of CCR4 expression with TH2 cells, the predominance of CCR4+ cells in blood from patients with AD, and an important role of CCR4 in the migration of TH2 cells from blood into AD lesional skin. (J Allergy Clin Immunol 2001;107:353-8.)</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1067/mai.2001.112601</identifier><identifier>PMID: 11174204</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Allergic diseases ; Atopic dermatitis ; Biological and medical sciences ; CCR4 ; CD4-Positive T-Lymphocytes - cytology ; CD4-Positive T-Lymphocytes - immunology ; Cell Movement ; chemokine receptors ; CXCR3 ; Dermatitis, Atopic - blood ; Humans ; Immunologic Memory ; Immunopathology ; inflammation ; Leukocyte Common Antigens - analysis ; Lymphocyte Count ; Medical sciences ; Receptors, CCR4 ; Receptors, Chemokine - biosynthesis ; Receptors, Chemokine - immunology ; Respiratory and ent allergic diseases ; skin ; Skin - pathology ; TH2 cells ; Th2 Cells - cytology</subject><ispartof>Journal of allergy and clinical immunology, 2001-02, Vol.107 (2), p.353-358</ispartof><rights>2001 Mosby, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-bd3b19cd1ee53036387f9076e4e21f36277002b2d71644323e997e13a8344f9c3</citedby><cites>FETCH-LOGICAL-c412t-bd3b19cd1ee53036387f9076e4e21f36277002b2d71644323e997e13a8344f9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1067/mai.2001.112601$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=891408$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11174204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakatani, Tomomi</creatorcontrib><creatorcontrib>Kaburagi, Yuko</creatorcontrib><creatorcontrib>Shimada, Yuka</creatorcontrib><creatorcontrib>Inaoki, Makoto</creatorcontrib><creatorcontrib>Takehara, Kazuhiko</creatorcontrib><creatorcontrib>Mukaida, Naofumi</creatorcontrib><creatorcontrib>Sato, Shinichi</creatorcontrib><title>CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>Background: Recent studies have reported that TH1 and TH2 cells express CXCR3 and CCR4, respectively. Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with TH2 and TH1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatitis (AD). Methods: Intracellular cytokine production and chemokine receptor expression in blood T cells were examined by flow cytometry. Immunohistochemical expression of chemokine receptors was also investigated in chronically lesional skin. Results: CCR4+ and CXCR3+ CD4+ T cells predominantly produced IL-4 and IFN-γ, respectively. Although the frequency of CXCR3+ cells among CD4+ CD45RO+ T cells was similar for patients with AD (n = 29) and healthy control subjects (n = 19), patients with severe AD (n = 14) had a reduced frequency of CXCR3+ cells. In contrast, the frequency of CCR4+ cells and the CCR4/CXCR3 ratio were higher in patients with AD (n = 22) than healthy control subjects (n = 16) and correlated with disease severity of AD. The frequency of CCR4+ cells correlated positively with eosinophil numbers and serum IgE levels, whereas the frequency of CXCR3+ cells correlated inversely with eosinophil numbers. The frequency of CCR4+ or CXCR3+ cells was similar in patients with psoriasis (n = 6) and healthy control subjects. Immunohistochemical analysis showed that the frequency of CCR4+ cells among CD4+ T cells in chronically lesional skin of patients with AD (n = 9) was higher than that of patients with psoriasis (n = 4). Conclusion: Our data suggest the association of CCR4 expression with TH2 cells, the predominance of CCR4+ cells in blood from patients with AD, and an important role of CCR4 in the migration of TH2 cells from blood into AD lesional skin. (J Allergy Clin Immunol 2001;107:353-8.)</description><subject>Allergic diseases</subject><subject>Atopic dermatitis</subject><subject>Biological and medical sciences</subject><subject>CCR4</subject><subject>CD4-Positive T-Lymphocytes - cytology</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>Cell Movement</subject><subject>chemokine receptors</subject><subject>CXCR3</subject><subject>Dermatitis, Atopic - blood</subject><subject>Humans</subject><subject>Immunologic Memory</subject><subject>Immunopathology</subject><subject>inflammation</subject><subject>Leukocyte Common Antigens - analysis</subject><subject>Lymphocyte Count</subject><subject>Medical sciences</subject><subject>Receptors, CCR4</subject><subject>Receptors, Chemokine - biosynthesis</subject><subject>Receptors, Chemokine - immunology</subject><subject>Respiratory and ent allergic diseases</subject><subject>skin</subject><subject>Skin - pathology</subject><subject>TH2 cells</subject><subject>Th2 Cells - cytology</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFrFTEQh4Mo9rV69iYBwYvsayZJk81RVq1CQSj1HLLJLC-6u1mTfdb33zflPfTU0_xm-GYYPkLeANsCU_pycnHLGYMtAFcMnpENMKMb1fKr52TDmIFGaWnOyHkpP1ntRWtekjMA0JIzuSF_u-5WfqATTikfaPep5js6HqZll_xhxUJdRhpnn9EVDDXRBXNcdpjdSPsxpUDdHOiIJaa5jsqvigw5TXRxa8R5LfQ-rjvq1rRETwPmqc7XWF6RF4MbC74-1Qvy48vnu-5rc_P9-lv38abxEvja9EH0YHwAxCvBhBKtHgzTCiVyGITiWjPGex40KCkFF2iMRhCuFVIOxosL8v54d8np9x7LaqdYPI6jmzHti9VMcQDFKnh5BH1OpWQc7JLj5PLBArOPsm2VbR9l26PsuvH2dHrfTxj-8ye7FXh3Alzxbhyym30s_7jWgGRtpcyRwqrhT8Rsi6_mPIaY0a82pPjkCw-IJJld</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Nakatani, Tomomi</creator><creator>Kaburagi, Yuko</creator><creator>Shimada, Yuka</creator><creator>Inaoki, Makoto</creator><creator>Takehara, Kazuhiko</creator><creator>Mukaida, Naofumi</creator><creator>Sato, Shinichi</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis</title><author>Nakatani, Tomomi ; Kaburagi, Yuko ; Shimada, Yuka ; Inaoki, Makoto ; Takehara, Kazuhiko ; Mukaida, Naofumi ; Sato, Shinichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-bd3b19cd1ee53036387f9076e4e21f36277002b2d71644323e997e13a8344f9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Allergic diseases</topic><topic>Atopic dermatitis</topic><topic>Biological and medical sciences</topic><topic>CCR4</topic><topic>CD4-Positive T-Lymphocytes - cytology</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>Cell Movement</topic><topic>chemokine receptors</topic><topic>CXCR3</topic><topic>Dermatitis, Atopic - blood</topic><topic>Humans</topic><topic>Immunologic Memory</topic><topic>Immunopathology</topic><topic>inflammation</topic><topic>Leukocyte Common Antigens - analysis</topic><topic>Lymphocyte Count</topic><topic>Medical sciences</topic><topic>Receptors, CCR4</topic><topic>Receptors, Chemokine - biosynthesis</topic><topic>Receptors, Chemokine - immunology</topic><topic>Respiratory and ent allergic diseases</topic><topic>skin</topic><topic>Skin - pathology</topic><topic>TH2 cells</topic><topic>Th2 Cells - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakatani, Tomomi</creatorcontrib><creatorcontrib>Kaburagi, Yuko</creatorcontrib><creatorcontrib>Shimada, Yuka</creatorcontrib><creatorcontrib>Inaoki, Makoto</creatorcontrib><creatorcontrib>Takehara, Kazuhiko</creatorcontrib><creatorcontrib>Mukaida, Naofumi</creatorcontrib><creatorcontrib>Sato, Shinichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakatani, Tomomi</au><au>Kaburagi, Yuko</au><au>Shimada, Yuka</au><au>Inaoki, Makoto</au><au>Takehara, Kazuhiko</au><au>Mukaida, Naofumi</au><au>Sato, Shinichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>107</volume><issue>2</issue><spage>353</spage><epage>358</epage><pages>353-358</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><coden>JACIBY</coden><abstract>Background: Recent studies have reported that TH1 and TH2 cells express CXCR3 and CCR4, respectively. Objective: Our goal was to assess the association of CCR4 and CXCR3 expression with TH2 and TH1 cells and association of CCR4 and CXCR3 expression with inflammation in patients with atopic dermatitis (AD). Methods: Intracellular cytokine production and chemokine receptor expression in blood T cells were examined by flow cytometry. Immunohistochemical expression of chemokine receptors was also investigated in chronically lesional skin. Results: CCR4+ and CXCR3+ CD4+ T cells predominantly produced IL-4 and IFN-γ, respectively. Although the frequency of CXCR3+ cells among CD4+ CD45RO+ T cells was similar for patients with AD (n = 29) and healthy control subjects (n = 19), patients with severe AD (n = 14) had a reduced frequency of CXCR3+ cells. In contrast, the frequency of CCR4+ cells and the CCR4/CXCR3 ratio were higher in patients with AD (n = 22) than healthy control subjects (n = 16) and correlated with disease severity of AD. The frequency of CCR4+ cells correlated positively with eosinophil numbers and serum IgE levels, whereas the frequency of CXCR3+ cells correlated inversely with eosinophil numbers. The frequency of CCR4+ or CXCR3+ cells was similar in patients with psoriasis (n = 6) and healthy control subjects. Immunohistochemical analysis showed that the frequency of CCR4+ cells among CD4+ T cells in chronically lesional skin of patients with AD (n = 9) was higher than that of patients with psoriasis (n = 4). Conclusion: Our data suggest the association of CCR4 expression with TH2 cells, the predominance of CCR4+ cells in blood from patients with AD, and an important role of CCR4 in the migration of TH2 cells from blood into AD lesional skin. (J Allergy Clin Immunol 2001;107:353-8.)</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>11174204</pmid><doi>10.1067/mai.2001.112601</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Allergic diseases Atopic dermatitis Biological and medical sciences CCR4 CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - immunology Cell Movement chemokine receptors CXCR3 Dermatitis, Atopic - blood Humans Immunologic Memory Immunopathology inflammation Leukocyte Common Antigens - analysis Lymphocyte Count Medical sciences Receptors, CCR4 Receptors, Chemokine - biosynthesis Receptors, Chemokine - immunology Respiratory and ent allergic diseases skin Skin - pathology TH2 cells Th2 Cells - cytology |
| title | CCR4+ memory CD4+ T lymphocytes are increased in peripheral blood and lesional skin from patients with atopic dermatitis |
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