Phenolic-rich juice prevents DNA single-strand breakage and cytotoxicity caused by tert-butylhydroperoxide in U937 cells: the role of iron chelation

The antioxidant potential of phenolic compounds is generally linked to their ability to scavenge free radicals. However, in addition to their radical-scavenging activity, phenolic compounds can chelate metal ions, such as iron, to prevent their participation in Fenton-type reactions, which lead to t...

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Veröffentlicht in:The Journal of nutritional biochemistry 2007-07, Vol.18 (7), p.457-466
Hauptverfasser: Garcia-Alonso, Francisco J., Guidarelli, Andrea, Periago, María J.
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container_title The Journal of nutritional biochemistry
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creator Garcia-Alonso, Francisco J.
Guidarelli, Andrea
Periago, María J.
description The antioxidant potential of phenolic compounds is generally linked to their ability to scavenge free radicals. However, in addition to their radical-scavenging activity, phenolic compounds can chelate metal ions, such as iron, to prevent their participation in Fenton-type reactions, which lead to the formation of free radicals. The aim of the present study was to evaluate the ability of a phenolic-rich juice made from grapes, cherries and berries to protect human myeloid leukemia (U937) cells from oxidative stress caused by tert-butylhydroperoxide (tB-OOH). Preincubation of cells with extracts of the phenolic-rich juice at different concentrations (0–200 μM ferulic acid equivalents) for 3 h partially prevented cell death and abolished the DNA cleavage induced by tB-OOH. Moreover, when preincubating cells with the 100-μM juice extract (the dose that diminished cell death by around 50%), the partial prevention of tB-OOH-induced formation of reactive oxygen species (ROS) and mitochondrial permeability transition pore opening was observed. The radical scavenger antioxidant N, N′-diphenyl-1,4-phenylene-diamine (DPPD) and the intracellular iron chelator o-phenanthroline ( o-Phe) were also tested to know whether protective effects depended on radical-scavenging or iron-chelating activities. o-Phe prevented cell death, DNA cleavage and ROS generation, whereas DPPD only prevented cell death, suggesting that phenolics in the juice afforded protection against induced oxidative stress, most probably by means of an iron-chelating mechanism.
doi_str_mv 10.1016/j.jnutbio.2006.08.003
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However, in addition to their radical-scavenging activity, phenolic compounds can chelate metal ions, such as iron, to prevent their participation in Fenton-type reactions, which lead to the formation of free radicals. The aim of the present study was to evaluate the ability of a phenolic-rich juice made from grapes, cherries and berries to protect human myeloid leukemia (U937) cells from oxidative stress caused by tert-butylhydroperoxide (tB-OOH). Preincubation of cells with extracts of the phenolic-rich juice at different concentrations (0–200 μM ferulic acid equivalents) for 3 h partially prevented cell death and abolished the DNA cleavage induced by tB-OOH. Moreover, when preincubating cells with the 100-μM juice extract (the dose that diminished cell death by around 50%), the partial prevention of tB-OOH-induced formation of reactive oxygen species (ROS) and mitochondrial permeability transition pore opening was observed. The radical scavenger antioxidant N, N′-diphenyl-1,4-phenylene-diamine (DPPD) and the intracellular iron chelator o-phenanthroline ( o-Phe) were also tested to know whether protective effects depended on radical-scavenging or iron-chelating activities. o-Phe prevented cell death, DNA cleavage and ROS generation, whereas DPPD only prevented cell death, suggesting that phenolics in the juice afforded protection against induced oxidative stress, most probably by means of an iron-chelating mechanism.</description><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Beverages</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>chelating agents</subject><subject>cherries</subject><subject>Cytotoxicity</subject><subject>DNA Damage - drug effects</subject><subject>DNA fragmentation</subject><subject>DNA single-strand breakage</subject><subject>DNA, Neoplasm - drug effects</subject><subject>DNA, Single-Stranded - drug effects</subject><subject>Feeding. Feeding behavior</subject><subject>fortified foods</subject><subject>free radical scavengers</subject><subject>free radicals</subject><subject>Fruit</subject><subject>fruit extracts</subject><subject>fruit juices</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>grape juice</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Iron Chelating Agents - pharmacology</subject><subject>Iron chelators</subject><subject>myeloid leukemia</subject><subject>oxidative stress</subject><subject>phenolic compounds</subject><subject>Phenolics</subject><subject>Phenols - administration &amp; dosage</subject><subject>Phenols - pharmacology</subject><subject>Radical scavengers</subject><subject>reactive oxygen species</subject><subject>single-stranded DNA</subject><subject>single-stranded DNA breakage</subject><subject>small fruits</subject><subject>tert-Butylhydroperoxide</subject><subject>tert-Butylhydroperoxide - pharmacology</subject><subject>U937 Cells</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d-O1CAUBvDGaNx19RFUbvSu42kpBbwxm_VvslETnWtC4TDD2Ckj0I19Dx9YJjPJXnpFSH4HTr6vqp43sGqg6d_sVrtpzoMPqxagX4FYAdAH1WUjOK070fGH1SVIxupW9PSiepLSDgDajvWPq4uml5IzKi-rv9-3OIXRmzp6syW72Rskh4h3OOVE3n-9JslPmxHrlKOeLBki6l96g-R4MUsOOfzxxueFGD0nLGAhGWOuhzkv43axMRwwFmOR-ImsJeXE4DimtyRvkcQwIgmO-BgmYrY46uzD9LR65PSY8Nn5vKrWHz_8vPlc33779OXm-rY2naC57iXrqERqBdPMUOOwcZ0TFrlrBVpubUlqMLJ1UjocqOScO6TMmZY67AZ6Vb0-vXuI4feMKau9T8ft9IRhTopD30IHvEB2giaGlCI6dYh-r-OiGlDHOtROnetQxzoUCFXqKHMvzh_Mwx7t_dQ5_wJenYFORo-uZGx8uncSgDEuint5ck4HpTexmPWPFhoKwAXr2q6IdyeBJbA7j1El43EyaH1Ek5UN_j_L_gM2NrhQ</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Garcia-Alonso, Francisco J.</creator><creator>Guidarelli, Andrea</creator><creator>Periago, María J.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Phenolic-rich juice prevents DNA single-strand breakage and cytotoxicity caused by tert-butylhydroperoxide in U937 cells: the role of iron chelation</title><author>Garcia-Alonso, Francisco J. ; Guidarelli, Andrea ; Periago, María J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-695439e3d85a5c3cfe1f4f8de7f28ed7dd101bc92f99feb39777fe35fc23fe4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Beverages</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>chelating agents</topic><topic>cherries</topic><topic>Cytotoxicity</topic><topic>DNA Damage - drug effects</topic><topic>DNA fragmentation</topic><topic>DNA single-strand breakage</topic><topic>DNA, Neoplasm - drug effects</topic><topic>DNA, Single-Stranded - drug effects</topic><topic>Feeding. Feeding behavior</topic><topic>fortified foods</topic><topic>free radical scavengers</topic><topic>free radicals</topic><topic>Fruit</topic><topic>fruit extracts</topic><topic>fruit juices</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>grape juice</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Iron Chelating Agents - pharmacology</topic><topic>Iron chelators</topic><topic>myeloid leukemia</topic><topic>oxidative stress</topic><topic>phenolic compounds</topic><topic>Phenolics</topic><topic>Phenols - administration &amp; dosage</topic><topic>Phenols - pharmacology</topic><topic>Radical scavengers</topic><topic>reactive oxygen species</topic><topic>single-stranded DNA</topic><topic>single-stranded DNA breakage</topic><topic>small fruits</topic><topic>tert-Butylhydroperoxide</topic><topic>tert-Butylhydroperoxide - pharmacology</topic><topic>U937 Cells</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia-Alonso, Francisco J.</creatorcontrib><creatorcontrib>Guidarelli, Andrea</creatorcontrib><creatorcontrib>Periago, María J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia-Alonso, Francisco J.</au><au>Guidarelli, Andrea</au><au>Periago, María J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenolic-rich juice prevents DNA single-strand breakage and cytotoxicity caused by tert-butylhydroperoxide in U937 cells: the role of iron chelation</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>18</volume><issue>7</issue><spage>457</spage><epage>466</epage><pages>457-466</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>The antioxidant potential of phenolic compounds is generally linked to their ability to scavenge free radicals. However, in addition to their radical-scavenging activity, phenolic compounds can chelate metal ions, such as iron, to prevent their participation in Fenton-type reactions, which lead to the formation of free radicals. The aim of the present study was to evaluate the ability of a phenolic-rich juice made from grapes, cherries and berries to protect human myeloid leukemia (U937) cells from oxidative stress caused by tert-butylhydroperoxide (tB-OOH). Preincubation of cells with extracts of the phenolic-rich juice at different concentrations (0–200 μM ferulic acid equivalents) for 3 h partially prevented cell death and abolished the DNA cleavage induced by tB-OOH. Moreover, when preincubating cells with the 100-μM juice extract (the dose that diminished cell death by around 50%), the partial prevention of tB-OOH-induced formation of reactive oxygen species (ROS) and mitochondrial permeability transition pore opening was observed. The radical scavenger antioxidant N, N′-diphenyl-1,4-phenylene-diamine (DPPD) and the intracellular iron chelator o-phenanthroline ( o-Phe) were also tested to know whether protective effects depended on radical-scavenging or iron-chelating activities. o-Phe prevented cell death, DNA cleavage and ROS generation, whereas DPPD only prevented cell death, suggesting that phenolics in the juice afforded protection against induced oxidative stress, most probably by means of an iron-chelating mechanism.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>16997539</pmid><doi>10.1016/j.jnutbio.2006.08.003</doi><tpages>10</tpages></addata></record>
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subjects antioxidant activity
antioxidants
Beverages
Biological and medical sciences
Cell Line, Tumor
chelating agents
cherries
Cytotoxicity
DNA Damage - drug effects
DNA fragmentation
DNA single-strand breakage
DNA, Neoplasm - drug effects
DNA, Single-Stranded - drug effects
Feeding. Feeding behavior
fortified foods
free radical scavengers
free radicals
Fruit
fruit extracts
fruit juices
Fundamental and applied biological sciences. Psychology
grape juice
human cell lines
Humans
Iron Chelating Agents - pharmacology
Iron chelators
myeloid leukemia
oxidative stress
phenolic compounds
Phenolics
Phenols - administration & dosage
Phenols - pharmacology
Radical scavengers
reactive oxygen species
single-stranded DNA
single-stranded DNA breakage
small fruits
tert-Butylhydroperoxide
tert-Butylhydroperoxide - pharmacology
U937 Cells
Vertebrates: anatomy and physiology, studies on body, several organs or systems
title Phenolic-rich juice prevents DNA single-strand breakage and cytotoxicity caused by tert-butylhydroperoxide in U937 cells: the role of iron chelation
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