Simultaneous Detection and Genogroup-Screening Test for Norovirus Genogroups I and II from Fecal Specimens in Single Tube by Reverse Transcription-Loop-Mediated Isothermal Amplification Assay
We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous...
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| Veröffentlicht in: | Microbiology and immunology 2007-01, Vol.51 (5), p.547-550 |
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| creator | Fukuda, Shinji Sasaki, Yukie Kuwayama, Masaru Miyazaki, Kazuo |
| description | We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence‐labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light at 312 nm. |
| doi_str_mv | 10.1111/j.1348-0421.2007.tb03932.x |
| format | Article |
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Sasaki, Yukie ; Kuwayama, Masaru ; Miyazaki, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5192-b7a84b87f720b41c9c6f03fb36f519af77a7af8f18bfa83932ed2b320b49a0c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Caliciviridae Infections - diagnosis</topic><topic>Caliciviridae Infections - epidemiology</topic><topic>Caliciviridae Infections - virology</topic><topic>Disease Outbreaks</topic><topic>DNA Primers - chemistry</topic><topic>DNA Primers - genetics</topic><topic>Feces - virology</topic><topic>Gastroenteritis - diagnosis</topic><topic>Gastroenteritis - epidemiology</topic><topic>Gastroenteritis - virology</topic><topic>genogrouping</topic><topic>Genotype</topic><topic>Humans</topic><topic>Norovirus</topic><topic>Norovirus - genetics</topic><topic>Norovirus - isolation & purification</topic><topic>NoV</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Reverse Transcription</topic><topic>RNA, Viral - chemistry</topic><topic>RNA, Viral - genetics</topic><topic>RT-LAMP</topic><topic>Sensitivity and Specificity</topic><topic>simultaneous detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukuda, Shinji</creatorcontrib><creatorcontrib>Sasaki, Yukie</creatorcontrib><creatorcontrib>Kuwayama, Masaru</creatorcontrib><creatorcontrib>Miyazaki, Kazuo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukuda, Shinji</au><au>Sasaki, Yukie</au><au>Kuwayama, Masaru</au><au>Miyazaki, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Detection and Genogroup-Screening Test for Norovirus Genogroups I and II from Fecal Specimens in Single Tube by Reverse Transcription-Loop-Mediated Isothermal Amplification Assay</atitle><jtitle>Microbiology and immunology</jtitle><addtitle>Microbiology and Immunology</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>51</volume><issue>5</issue><spage>547</spage><epage>550</epage><pages>547-550</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><abstract>We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence‐labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light at 312 nm.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>17579265</pmid><doi>10.1111/j.1348-0421.2007.tb03932.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Open Access Titles of Japan; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Caliciviridae Infections - diagnosis Caliciviridae Infections - epidemiology Caliciviridae Infections - virology Disease Outbreaks DNA Primers - chemistry DNA Primers - genetics Feces - virology Gastroenteritis - diagnosis Gastroenteritis - epidemiology Gastroenteritis - virology genogrouping Genotype Humans Norovirus Norovirus - genetics Norovirus - isolation & purification NoV Nucleic Acid Amplification Techniques - methods Reverse Transcription RNA, Viral - chemistry RNA, Viral - genetics RT-LAMP Sensitivity and Specificity simultaneous detection |
| title | Simultaneous Detection and Genogroup-Screening Test for Norovirus Genogroups I and II from Fecal Specimens in Single Tube by Reverse Transcription-Loop-Mediated Isothermal Amplification Assay |
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