Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster

Eggs of the newt, Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction co...

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Veröffentlicht in:	Developmental biology 2007-06, Vol.306 (2), p.797-808
Hauptverfasser:	Harada, Yuichirou, Matsumoto, Tamami, Hirahara, Shino, Nakashima, Akira, Ueno, Shuichi, Oda, Shoji, Miyazaki, Shunichi, Iwao, Yasuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosome Reaction Amphibian Animals Calcium - metabolism Citrate (si)-Synthase - genetics Citrate (si)-Synthase - metabolism Citrate (si)-Synthase - physiology Citrate synthase Cynops pyrrhogaster Egg activation Fertilization Freshwater Male Mice Microscopy, Fluorescence PLCζ RNA, Messenger - metabolism Salamandridae Spermatozoa - metabolism Swine Time Factors Type C Phospholipases - metabolism Xenopus
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