Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster
Eggs of the newt, Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction co...
Gespeichert in:
| Veröffentlicht in: | Developmental biology 2007-06, Vol.306 (2), p.797-808 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 808 |
|---|---|
| container_issue | 2 |
| container_start_page | 797 |
| container_title | Developmental biology |
| container_volume | 306 |
| creator | Harada, Yuichirou Matsumoto, Tamami Hirahara, Shino Nakashima, Akira Ueno, Shuichi Oda, Shoji Miyazaki, Shunichi Iwao, Yasuhiro |
| description | Eggs of the newt,
Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction containing only a 45 kDa protein induced egg activation accompanied by an intracellular Ca
2+ increase when injected into unfertilized eggs. Although injection of mouse phospholipase C (PLC) ζ-mRNA caused a Ca
2+ increase and egg activation, partial amino acid sequences of the 45 kDa protein were homologous to those of
Xenopus citrate synthase, but not to PLCs. An anti-porcine citrate synthase antibody recognized the 45 kDa protein both in the purified fraction and in the sperm extract. Treatment with the anti-citrate synthase antibody reduced the egg-activation activity in the sperm extract. Injection of porcine citrate synthase or mRNA of
Xenopus citrate synthase induced a Ca
2+ increase and caused egg activation. A large amount of the 45 kDa protein was localized in two lines elongated from the neck to the middle piece of sperm. These results indicate that the 45 kDa protein is a major component of the sperm factor for egg activation at newt fertilization. |
| doi_str_mv | 10.1016/j.ydbio.2007.04.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70616443</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0012160607007944</els_id><sourcerecordid>20749581</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-4792d18a7fe1c73239b1e3d72174c554fdae30643aaa3271f8f98385b5b34153</originalsourceid><addsrcrecordid>eNqFkUFrGzEQhUVoqJ20vyAQdOptN6OVdrV76KGYtA0EcvEhN6HVjmwZe7WRZBfn11epDe2pOQgh9L03w3uE3DAoGbDmblMeh975sgKQJYgSWHdB5gy6uqgb8fyBzAFYVbAGmhm5inEDALxt-UcyY1J0nQSYE7tY66BNwuBedXJ-pN5STeOEYUdt_vCB2nxwtaL55Q4nSCdqMSS3_UeV1khH_JXo4jj6KdLpGMLar3TM5p_IpdXbiJ_P9zVZfr9fLn4Wj08_HhbfHguTF0qFkF01sFZLi8xIXvGuZ8gHWeWFTV0LO2jk0AiuteaVZLa1Xcvbuq97LljNr8mXk-0U_MseY1I7Fw1ut3pEv49KQsMaIfi7YAU5obplGeQn0AQfY0CrpuB2OhwVA_VWg9qoPzWotxoUCJVryKrbs_2-3-HwV3POPQNfTwDmMA4Og4rG4WhwcAFNUoN3_x3wGw-smuE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20749581</pqid></control><display><type>article</type><title>Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Harada, Yuichirou ; Matsumoto, Tamami ; Hirahara, Shino ; Nakashima, Akira ; Ueno, Shuichi ; Oda, Shoji ; Miyazaki, Shunichi ; Iwao, Yasuhiro</creator><creatorcontrib>Harada, Yuichirou ; Matsumoto, Tamami ; Hirahara, Shino ; Nakashima, Akira ; Ueno, Shuichi ; Oda, Shoji ; Miyazaki, Shunichi ; Iwao, Yasuhiro</creatorcontrib><description>Eggs of the newt,
Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction containing only a 45 kDa protein induced egg activation accompanied by an intracellular Ca
2+ increase when injected into unfertilized eggs. Although injection of mouse phospholipase C (PLC) ζ-mRNA caused a Ca
2+ increase and egg activation, partial amino acid sequences of the 45 kDa protein were homologous to those of
Xenopus citrate synthase, but not to PLCs. An anti-porcine citrate synthase antibody recognized the 45 kDa protein both in the purified fraction and in the sperm extract. Treatment with the anti-citrate synthase antibody reduced the egg-activation activity in the sperm extract. Injection of porcine citrate synthase or mRNA of
Xenopus citrate synthase induced a Ca
2+ increase and caused egg activation. A large amount of the 45 kDa protein was localized in two lines elongated from the neck to the middle piece of sperm. These results indicate that the 45 kDa protein is a major component of the sperm factor for egg activation at newt fertilization.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2007.04.019</identifier><identifier>PMID: 17499700</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrosome Reaction ; Amphibian ; Animals ; Calcium - metabolism ; Citrate (si)-Synthase - genetics ; Citrate (si)-Synthase - metabolism ; Citrate (si)-Synthase - physiology ; Citrate synthase ; Cynops pyrrhogaster ; Egg activation ; Fertilization ; Freshwater ; Male ; Mice ; Microscopy, Fluorescence ; PLCζ ; RNA, Messenger - metabolism ; Salamandridae ; Spermatozoa - metabolism ; Swine ; Time Factors ; Type C Phospholipases - metabolism ; Xenopus</subject><ispartof>Developmental biology, 2007-06, Vol.306 (2), p.797-808</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-4792d18a7fe1c73239b1e3d72174c554fdae30643aaa3271f8f98385b5b34153</citedby><cites>FETCH-LOGICAL-c499t-4792d18a7fe1c73239b1e3d72174c554fdae30643aaa3271f8f98385b5b34153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ydbio.2007.04.019$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17499700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harada, Yuichirou</creatorcontrib><creatorcontrib>Matsumoto, Tamami</creatorcontrib><creatorcontrib>Hirahara, Shino</creatorcontrib><creatorcontrib>Nakashima, Akira</creatorcontrib><creatorcontrib>Ueno, Shuichi</creatorcontrib><creatorcontrib>Oda, Shoji</creatorcontrib><creatorcontrib>Miyazaki, Shunichi</creatorcontrib><creatorcontrib>Iwao, Yasuhiro</creatorcontrib><title>Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Eggs of the newt,
Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction containing only a 45 kDa protein induced egg activation accompanied by an intracellular Ca
2+ increase when injected into unfertilized eggs. Although injection of mouse phospholipase C (PLC) ζ-mRNA caused a Ca
2+ increase and egg activation, partial amino acid sequences of the 45 kDa protein were homologous to those of
Xenopus citrate synthase, but not to PLCs. An anti-porcine citrate synthase antibody recognized the 45 kDa protein both in the purified fraction and in the sperm extract. Treatment with the anti-citrate synthase antibody reduced the egg-activation activity in the sperm extract. Injection of porcine citrate synthase or mRNA of
Xenopus citrate synthase induced a Ca
2+ increase and caused egg activation. A large amount of the 45 kDa protein was localized in two lines elongated from the neck to the middle piece of sperm. These results indicate that the 45 kDa protein is a major component of the sperm factor for egg activation at newt fertilization.</description><subject>Acrosome Reaction</subject><subject>Amphibian</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Citrate (si)-Synthase - genetics</subject><subject>Citrate (si)-Synthase - metabolism</subject><subject>Citrate (si)-Synthase - physiology</subject><subject>Citrate synthase</subject><subject>Cynops pyrrhogaster</subject><subject>Egg activation</subject><subject>Fertilization</subject><subject>Freshwater</subject><subject>Male</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>PLCζ</subject><subject>RNA, Messenger - metabolism</subject><subject>Salamandridae</subject><subject>Spermatozoa - metabolism</subject><subject>Swine</subject><subject>Time Factors</subject><subject>Type C Phospholipases - metabolism</subject><subject>Xenopus</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFrGzEQhUVoqJ20vyAQdOptN6OVdrV76KGYtA0EcvEhN6HVjmwZe7WRZBfn11epDe2pOQgh9L03w3uE3DAoGbDmblMeh975sgKQJYgSWHdB5gy6uqgb8fyBzAFYVbAGmhm5inEDALxt-UcyY1J0nQSYE7tY66BNwuBedXJ-pN5STeOEYUdt_vCB2nxwtaL55Q4nSCdqMSS3_UeV1khH_JXo4jj6KdLpGMLar3TM5p_IpdXbiJ_P9zVZfr9fLn4Wj08_HhbfHguTF0qFkF01sFZLi8xIXvGuZ8gHWeWFTV0LO2jk0AiuteaVZLa1Xcvbuq97LljNr8mXk-0U_MseY1I7Fw1ut3pEv49KQsMaIfi7YAU5obplGeQn0AQfY0CrpuB2OhwVA_VWg9qoPzWotxoUCJVryKrbs_2-3-HwV3POPQNfTwDmMA4Og4rG4WhwcAFNUoN3_x3wGw-smuE</recordid><startdate>20070615</startdate><enddate>20070615</enddate><creator>Harada, Yuichirou</creator><creator>Matsumoto, Tamami</creator><creator>Hirahara, Shino</creator><creator>Nakashima, Akira</creator><creator>Ueno, Shuichi</creator><creator>Oda, Shoji</creator><creator>Miyazaki, Shunichi</creator><creator>Iwao, Yasuhiro</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070615</creationdate><title>Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster</title><author>Harada, Yuichirou ; Matsumoto, Tamami ; Hirahara, Shino ; Nakashima, Akira ; Ueno, Shuichi ; Oda, Shoji ; Miyazaki, Shunichi ; Iwao, Yasuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-4792d18a7fe1c73239b1e3d72174c554fdae30643aaa3271f8f98385b5b34153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acrosome Reaction</topic><topic>Amphibian</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Citrate (si)-Synthase - genetics</topic><topic>Citrate (si)-Synthase - metabolism</topic><topic>Citrate (si)-Synthase - physiology</topic><topic>Citrate synthase</topic><topic>Cynops pyrrhogaster</topic><topic>Egg activation</topic><topic>Fertilization</topic><topic>Freshwater</topic><topic>Male</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>PLCζ</topic><topic>RNA, Messenger - metabolism</topic><topic>Salamandridae</topic><topic>Spermatozoa - metabolism</topic><topic>Swine</topic><topic>Time Factors</topic><topic>Type C Phospholipases - metabolism</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harada, Yuichirou</creatorcontrib><creatorcontrib>Matsumoto, Tamami</creatorcontrib><creatorcontrib>Hirahara, Shino</creatorcontrib><creatorcontrib>Nakashima, Akira</creatorcontrib><creatorcontrib>Ueno, Shuichi</creatorcontrib><creatorcontrib>Oda, Shoji</creatorcontrib><creatorcontrib>Miyazaki, Shunichi</creatorcontrib><creatorcontrib>Iwao, Yasuhiro</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harada, Yuichirou</au><au>Matsumoto, Tamami</au><au>Hirahara, Shino</au><au>Nakashima, Akira</au><au>Ueno, Shuichi</au><au>Oda, Shoji</au><au>Miyazaki, Shunichi</au><au>Iwao, Yasuhiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2007-06-15</date><risdate>2007</risdate><volume>306</volume><issue>2</issue><spage>797</spage><epage>808</epage><pages>797-808</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>Eggs of the newt,
Cynops pyrrhogaster, arrested at the second meiotic metaphase are activated by sperm at fertilization and then complete meiosis to initiate development. We highly purified a sperm factor for egg activation from a sperm extract with several chromatographies. The purified fraction containing only a 45 kDa protein induced egg activation accompanied by an intracellular Ca
2+ increase when injected into unfertilized eggs. Although injection of mouse phospholipase C (PLC) ζ-mRNA caused a Ca
2+ increase and egg activation, partial amino acid sequences of the 45 kDa protein were homologous to those of
Xenopus citrate synthase, but not to PLCs. An anti-porcine citrate synthase antibody recognized the 45 kDa protein both in the purified fraction and in the sperm extract. Treatment with the anti-citrate synthase antibody reduced the egg-activation activity in the sperm extract. Injection of porcine citrate synthase or mRNA of
Xenopus citrate synthase induced a Ca
2+ increase and caused egg activation. A large amount of the 45 kDa protein was localized in two lines elongated from the neck to the middle piece of sperm. These results indicate that the 45 kDa protein is a major component of the sperm factor for egg activation at newt fertilization.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17499700</pmid><doi>10.1016/j.ydbio.2007.04.019</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 2007-06, Vol.306 (2), p.797-808 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70616443 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Acrosome Reaction Amphibian Animals Calcium - metabolism Citrate (si)-Synthase - genetics Citrate (si)-Synthase - metabolism Citrate (si)-Synthase - physiology Citrate synthase Cynops pyrrhogaster Egg activation Fertilization Freshwater Male Mice Microscopy, Fluorescence PLCζ RNA, Messenger - metabolism Salamandridae Spermatozoa - metabolism Swine Time Factors Type C Phospholipases - metabolism Xenopus |
| title | Characterization of a sperm factor for egg activation at fertilization of the newt Cynops pyrrhogaster |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T14%3A53%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20a%20sperm%20factor%20for%20egg%20activation%20at%20fertilization%20of%20the%20newt%20Cynops%20pyrrhogaster&rft.jtitle=Developmental%20biology&rft.au=Harada,%20Yuichirou&rft.date=2007-06-15&rft.volume=306&rft.issue=2&rft.spage=797&rft.epage=808&rft.pages=797-808&rft.issn=0012-1606&rft.eissn=1095-564X&rft_id=info:doi/10.1016/j.ydbio.2007.04.019&rft_dat=%3Cproquest_cross%3E20749581%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20749581&rft_id=info:pmid/17499700&rft_els_id=S0012160607007944&rfr_iscdi=true |