Gene analysis of genomic DNA from stored serum by polymerase chain reaction: identification of three missense mutations in patients with cholinesterasemia and ABO genotyping

We established a method to determine the butyrylcholinesterase genotype associated with a BCHE deficiency directly using multiple PCR from stored serum, which was stored at −70°C for more than 30 years. PCR products from sera of six propositi were used for DNA sequence analysis. All of these BChE va...

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