Golgi complex disassembly caused by light-activated Calphostin C involves MAPK and PKA
Abstract We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy...
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| Veröffentlicht in: | Tissue & cell 2007-06, Vol.39 (3), p.161-169 |
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| creator | Morgado-Díaz, J.A Montesano, G De Souza Fernandes, S Redondo, P.A Fernandes de Souza, W Albuquerque-Xavier, A.C Leve, F Tanaka, M.N Martins de Araujo, W Oliveira, S.S Benchimol, Marlene De Souza, W |
| description | Abstract We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells. |
| doi_str_mv | 10.1016/j.tice.2007.03.001 |
| format | Article |
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When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. 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When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells.</description><subject>Advanced Basic Science</subject><subject>Calphostin C</subject><subject>Cell signaling</subject><subject>Cyclic AMP-Dependent Protein Kinases - metabolism</subject><subject>Endocytosis - drug effects</subject><subject>Endocytosis - radiation effects</subject><subject>Flavonoids - pharmacology</subject><subject>Fluorescent Antibody Technique</subject><subject>Golgi Apparatus - drug effects</subject><subject>Golgi Apparatus - metabolism</subject><subject>Golgi Apparatus - radiation effects</subject><subject>Golgi Apparatus - ultrastructure</subject><subject>Golgi complex</subject><subject>Horseradish Peroxidase - metabolism</subject><subject>HT-29 cells</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Isoquinolines - pharmacology</subject><subject>Light</subject><subject>MAPK</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Naphthalenes - chemistry</subject><subject>Naphthalenes - pharmacology</subject><subject>Naphthalenes - radiation effects</subject><subject>PKA</subject><subject>Staurosporine - pharmacology</subject><subject>Sulfonamides - pharmacology</subject><issn>0040-8166</issn><issn>1532-3072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1r3DAURUVpaSZp_0AXRavu7D5JtmVDKQxDm5QkJNCPrZCl50RT2Zpa9tD595WZgUAWXT0Q517QuYS8Y5AzYNXHbT45gzkHkDmIHIC9ICtWCp4JkPwlWQEUkNWsqs7IeYxbSGDB5GtyxtLlooYV-XUZ_IOjJvQ7j3-pdVHHiH3rD9ToOaKl7YF69_A4ZdpMbq-n9LTRfvcY4uQGuqFu2Ae_x0hv1_fXVA-W3l-v35BXnfYR357uBfn59cuPzVV2c3f5bbO-yUwhyylrWF2bWneSN4LZoikQjBSal1xwbWxtbalbiUUnZcca1nXWmrLjgrGqq-umFRfkw7F3N4Y_M8ZJ9S4a9F4PGOaoJFRMVFAkkB9BM4YYR-zUbnS9Hg-KgVpsqq1abKrFpgKhks0Uen9qn9se7VPkpC8Bn44Apj_uHY4qGoeDQetGNJOywf2___OzuPFucEb733jAuA3zOCR7iqnIFajvy57LnCDTlJyX4h_gL5oq</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Morgado-Díaz, J.A</creator><creator>Montesano, G</creator><creator>De Souza Fernandes, S</creator><creator>Redondo, P.A</creator><creator>Fernandes de Souza, W</creator><creator>Albuquerque-Xavier, A.C</creator><creator>Leve, F</creator><creator>Tanaka, M.N</creator><creator>Martins de Araujo, W</creator><creator>Oliveira, S.S</creator><creator>Benchimol, Marlene</creator><creator>De Souza, W</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070601</creationdate><title>Golgi complex disassembly caused by light-activated Calphostin C involves MAPK and PKA</title><author>Morgado-Díaz, J.A ; 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When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>17412380</pmid><doi>10.1016/j.tice.2007.03.001</doi><tpages>9</tpages></addata></record> |
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| subjects | Advanced Basic Science Calphostin C Cell signaling Cyclic AMP-Dependent Protein Kinases - metabolism Endocytosis - drug effects Endocytosis - radiation effects Flavonoids - pharmacology Fluorescent Antibody Technique Golgi Apparatus - drug effects Golgi Apparatus - metabolism Golgi Apparatus - radiation effects Golgi Apparatus - ultrastructure Golgi complex Horseradish Peroxidase - metabolism HT-29 cells HT29 Cells Humans Isoquinolines - pharmacology Light MAPK Mitogen-Activated Protein Kinases - metabolism Naphthalenes - chemistry Naphthalenes - pharmacology Naphthalenes - radiation effects PKA Staurosporine - pharmacology Sulfonamides - pharmacology |
| title | Golgi complex disassembly caused by light-activated Calphostin C involves MAPK and PKA |
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