Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination
Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26–30) serum...
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| Veröffentlicht in: | The Journal of infectious diseases 2007-07, Vol.196 (2), p.220-229 |
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| creator | Lawrence, Steven J. Lottenbach, Kathleen R. Newman, Frances K. Buller, R. Mark L. Bellone, Clifford J. Chen, John J. Cohen, Gary H. Eisenberg, Roselyn J. Belshe, Robert B. Stanley, Samuel L. Frey, Sharon E. |
| description | Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26–30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P |
| doi_str_mv | 10.1086/518793 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_70611724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30086635</jstor_id><sourcerecordid>30086635</sourcerecordid><originalsourceid>FETCH-LOGICAL-c430t-e28a9cd81fa74ee0a93c73717d8dd6321a4beb7de840af04196d2df3ae81121e3</originalsourceid><addsrcrecordid>eNqF0E1P3DAQBmALUcFC6T9olQu9pfXYie0cEYJu1aWt-rFCvViOPZEMSbzYXgn-PUEbscee5vA-mhm9hLwD-gmoEp9rULLhB2QBNZelEMAPyYJSxkpQTXNMTlK6o5RWXMgjcgyylhRosyDfLsbs2-Ceil-YNmFMmIocirWx1o_eFDc4tNGMWPyMIaMfU2G6jLH4PZi-34THWZrsw_iWvOlMn_Bsnqfk7_XVn8tlufrx5evlxaq0Fae5RKZMY52CzsgKkZqGW8klSKecE5yBqVpspUNVUdPRChrhmOu4QQXAAPkp-bjbu4nhYYsp68Eni30__Rm2SUsqACSr_gsZrVkNUu2hjSGliJ3eRD-Y-KSB6pd-9a7fCX6YN27bAd2ezYVO4HwGJlnTd1N31qe9Uw3UUtaTe79zdymH-JpzOh0T_CUvd7lPGR9fcxPvtZjKqvXy9p9eXa-X39nNWkv-DNG6mhA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20525178</pqid></control><display><type>article</type><title>Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Lawrence, Steven J. ; Lottenbach, Kathleen R. ; Newman, Frances K. ; Buller, R. Mark L. ; Bellone, Clifford J. ; Chen, John J. ; Cohen, Gary H. ; Eisenberg, Roselyn J. ; Belshe, Robert B. ; Stanley, Samuel L. ; Frey, Sharon E.</creator><creatorcontrib>Lawrence, Steven J. ; Lottenbach, Kathleen R. ; Newman, Frances K. ; Buller, R. Mark L. ; Bellone, Clifford J. ; Chen, John J. ; Cohen, Gary H. ; Eisenberg, Roselyn J. ; Belshe, Robert B. ; Stanley, Samuel L. ; Frey, Sharon E.</creatorcontrib><description>Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26–30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P<.001), whereas anti-L1 antibody responses were less robust (detection rate, 31%; GMT, 75) in nonnaive subjects. Diluted vaccine elicited antibody responses that were similar to those elicited by undiluted vaccine. Conclusions. Vaccination with VV elicits long-lived specific antibody responses directed against VV membrane proteins that vary by previous vaccination status but not with respect to 10-fold dilution of vaccine. B5, A33, and A27 should be considered for inclusion in future human orthopoxvirus subunit vaccines.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/518793</identifier><identifier>PMID: 17570109</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adolescent ; Adult ; Antibodies ; Antibody Specificity - immunology ; Biological and medical sciences ; Enzyme linked immunosorbent assay ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunity ; Immunoglobulin G - analysis ; Male ; Membrane proteins ; Microbiology ; Monoclonal antibodies ; Orthopoxvirus ; Smallpox ; Smallpox Vaccine - immunology ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccinia ; Vaccinia virus ; Vaccinia virus - immunology ; Viral Envelope Proteins - immunology ; Virology ; Viruses</subject><ispartof>The Journal of infectious diseases, 2007-07, Vol.196 (2), p.220-229</ispartof><rights>Copyright 2007 Infectious Diseases Society of America</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-e28a9cd81fa74ee0a93c73717d8dd6321a4beb7de840af04196d2df3ae81121e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30086635$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30086635$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18915775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17570109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawrence, Steven J.</creatorcontrib><creatorcontrib>Lottenbach, Kathleen R.</creatorcontrib><creatorcontrib>Newman, Frances K.</creatorcontrib><creatorcontrib>Buller, R. Mark L.</creatorcontrib><creatorcontrib>Bellone, Clifford J.</creatorcontrib><creatorcontrib>Chen, John J.</creatorcontrib><creatorcontrib>Cohen, Gary H.</creatorcontrib><creatorcontrib>Eisenberg, Roselyn J.</creatorcontrib><creatorcontrib>Belshe, Robert B.</creatorcontrib><creatorcontrib>Stanley, Samuel L.</creatorcontrib><creatorcontrib>Frey, Sharon E.</creatorcontrib><title>Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26–30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P<.001), whereas anti-L1 antibody responses were less robust (detection rate, 31%; GMT, 75) in nonnaive subjects. Diluted vaccine elicited antibody responses that were similar to those elicited by undiluted vaccine. Conclusions. Vaccination with VV elicits long-lived specific antibody responses directed against VV membrane proteins that vary by previous vaccination status but not with respect to 10-fold dilution of vaccine. B5, A33, and A27 should be considered for inclusion in future human orthopoxvirus subunit vaccines.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antibodies</subject><subject>Antibody Specificity - immunology</subject><subject>Biological and medical sciences</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunity</subject><subject>Immunoglobulin G - analysis</subject><subject>Male</subject><subject>Membrane proteins</subject><subject>Microbiology</subject><subject>Monoclonal antibodies</subject><subject>Orthopoxvirus</subject><subject>Smallpox</subject><subject>Smallpox Vaccine - immunology</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccinia</subject><subject>Vaccinia virus</subject><subject>Vaccinia virus - immunology</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Virology</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1P3DAQBmALUcFC6T9olQu9pfXYie0cEYJu1aWt-rFCvViOPZEMSbzYXgn-PUEbscee5vA-mhm9hLwD-gmoEp9rULLhB2QBNZelEMAPyYJSxkpQTXNMTlK6o5RWXMgjcgyylhRosyDfLsbs2-Ceil-YNmFMmIocirWx1o_eFDc4tNGMWPyMIaMfU2G6jLH4PZi-34THWZrsw_iWvOlMn_Bsnqfk7_XVn8tlufrx5evlxaq0Fae5RKZMY52CzsgKkZqGW8klSKecE5yBqVpspUNVUdPRChrhmOu4QQXAAPkp-bjbu4nhYYsp68Eni30__Rm2SUsqACSr_gsZrVkNUu2hjSGliJ3eRD-Y-KSB6pd-9a7fCX6YN27bAd2ezYVO4HwGJlnTd1N31qe9Uw3UUtaTe79zdymH-JpzOh0T_CUvd7lPGR9fcxPvtZjKqvXy9p9eXa-X39nNWkv-DNG6mhA</recordid><startdate>20070715</startdate><enddate>20070715</enddate><creator>Lawrence, Steven J.</creator><creator>Lottenbach, Kathleen R.</creator><creator>Newman, Frances K.</creator><creator>Buller, R. Mark L.</creator><creator>Bellone, Clifford J.</creator><creator>Chen, John J.</creator><creator>Cohen, Gary H.</creator><creator>Eisenberg, Roselyn J.</creator><creator>Belshe, Robert B.</creator><creator>Stanley, Samuel L.</creator><creator>Frey, Sharon E.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20070715</creationdate><title>Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination</title><author>Lawrence, Steven J. ; Lottenbach, Kathleen R. ; Newman, Frances K. ; Buller, R. Mark L. ; Bellone, Clifford J. ; Chen, John J. ; Cohen, Gary H. ; Eisenberg, Roselyn J. ; Belshe, Robert B. ; Stanley, Samuel L. ; Frey, Sharon E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-e28a9cd81fa74ee0a93c73717d8dd6321a4beb7de840af04196d2df3ae81121e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Antibodies</topic><topic>Antibody Specificity - immunology</topic><topic>Biological and medical sciences</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunity</topic><topic>Immunoglobulin G - analysis</topic><topic>Male</topic><topic>Membrane proteins</topic><topic>Microbiology</topic><topic>Monoclonal antibodies</topic><topic>Orthopoxvirus</topic><topic>Smallpox</topic><topic>Smallpox Vaccine - immunology</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccinia</topic><topic>Vaccinia virus</topic><topic>Vaccinia virus - immunology</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Virology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lawrence, Steven J.</creatorcontrib><creatorcontrib>Lottenbach, Kathleen R.</creatorcontrib><creatorcontrib>Newman, Frances K.</creatorcontrib><creatorcontrib>Buller, R. Mark L.</creatorcontrib><creatorcontrib>Bellone, Clifford J.</creatorcontrib><creatorcontrib>Chen, John J.</creatorcontrib><creatorcontrib>Cohen, Gary H.</creatorcontrib><creatorcontrib>Eisenberg, Roselyn J.</creatorcontrib><creatorcontrib>Belshe, Robert B.</creatorcontrib><creatorcontrib>Stanley, Samuel L.</creatorcontrib><creatorcontrib>Frey, Sharon E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawrence, Steven J.</au><au>Lottenbach, Kathleen R.</au><au>Newman, Frances K.</au><au>Buller, R. Mark L.</au><au>Bellone, Clifford J.</au><au>Chen, John J.</au><au>Cohen, Gary H.</au><au>Eisenberg, Roselyn J.</au><au>Belshe, Robert B.</au><au>Stanley, Samuel L.</au><au>Frey, Sharon E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2007-07-15</date><risdate>2007</risdate><volume>196</volume><issue>2</issue><spage>220</spage><epage>229</epage><pages>220-229</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Background. Vaccinia virus (VV) membrane proteins are candidates for orthopoxvirus subunit vaccines and potential targets for therapeutic antibodies. Human antibody responses to these proteins after VV vaccination have not been well characterized. Methods. Pre- and postvaccination (day 26–30) serum specimens from 80 VV vaccine recipients were examined for immunoglobulin G antibodies specific for B5, A33, A27, and L1 by enzyme-linked immunosorbent assay (ELISA). Responses were compared between vaccinia-naive and previously vaccinated (nonnaive) recipients and between nonnaive recipients of undiluted or 1:10 diluted vaccine. Results. VV vaccination elicited anti-A33 and anti-A27 antibodies in nearly all vaccinia-naive subjects (100% and 93%, respectively). Preexisting antibodies were commonly detected in nonnaive subjects (for anti-B5, 68%; for anti-A33, 59%; for anti-A27, 38%; and for anti-L1, 10%). Anti-B5 antibodies were strongly boosted by undiluted vaccine (geometric mean titer [GMT], 151 vs. 1010 for pre- vs. postvaccination; P<.001), whereas anti-L1 antibody responses were less robust (detection rate, 31%; GMT, 75) in nonnaive subjects. Diluted vaccine elicited antibody responses that were similar to those elicited by undiluted vaccine. Conclusions. Vaccination with VV elicits long-lived specific antibody responses directed against VV membrane proteins that vary by previous vaccination status but not with respect to 10-fold dilution of vaccine. B5, A33, and A27 should be considered for inclusion in future human orthopoxvirus subunit vaccines.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>17570109</pmid><doi>10.1086/518793</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Antibodies Antibody Specificity - immunology Biological and medical sciences Enzyme linked immunosorbent assay Female Fundamental and applied biological sciences. Psychology Humans Immunity Immunoglobulin G - analysis Male Membrane proteins Microbiology Monoclonal antibodies Orthopoxvirus Smallpox Smallpox Vaccine - immunology Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccinia Vaccinia virus Vaccinia virus - immunology Viral Envelope Proteins - immunology Virology Viruses |
| title | Antibody Responses to Vaccinia Membrane Proteins after Smallpox Vaccination |
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