Thermostable collagenolytic activity of a novel thermophilic isolate, Bacillus sp. strain NTAP-1
We isolated an acidophilic thermophile belonging to the genus Bacillus, strain NTAP-1, which secreted a thermostable collagenolytic activity into the culture medium. The collagenolytic activity exhibited an optimum pH for Azocoll hydrolysis of pH 3.9 and was not completely inhibited by 10 mM ethylen...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2000, Vol.89 (6), p.612-614 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Nakayama, Toru Tsuruoka, Naoki Akai, Minoru Nishino, Tokuzo |
| description | We isolated an acidophilic thermophile belonging to the genus
Bacillus, strain NTAP-1, which secreted a thermostable collagenolytic activity into the culture medium. The collagenolytic activity exhibited an optimum pH for Azocoll hydrolysis of pH 3.9 and was not completely inhibited by 10 mM ethylenediaminetetraacetic acid (residual activity, 63%), suggesting that
Bacillus NTAP-1 produces a novel acid proteinase with highest activity for collagen. The collagenolytic activity was thermostable; more than 80% of the original activity was retained after incubation of the culture supernatant at pH 4.0 and 60°C for 4 h. |
| doi_str_mv | 10.1016/S1389-1723(00)80067-5 |
| format | Article |
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Bacillus, strain NTAP-1, which secreted a thermostable collagenolytic activity into the culture medium. The collagenolytic activity exhibited an optimum pH for Azocoll hydrolysis of pH 3.9 and was not completely inhibited by 10 mM ethylenediaminetetraacetic acid (residual activity, 63%), suggesting that
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| ispartof | Journal of bioscience and bioengineering, 2000, Vol.89 (6), p.612-614 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | acidophile Acids Bacillus Bacillus sp Biodegradation Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cell culture Collagen collagenase Enzymes Fundamental and applied biological sciences. Psychology gelatin Hydrolysis Miscellaneous Mission oriented research Thermodynamic stability thermostability |
| title | Thermostable collagenolytic activity of a novel thermophilic isolate, Bacillus sp. strain NTAP-1 |
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