Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering
The objective of the present study was to synthesize and characterize chitosans with different degrees of deacetylation (DDA%), prepare chitosan microspheres with controlled chemistry and geometry, and fabricate three-dimensional (3-D) chitosan matrices based on microspheres with appropriate pore si...
Gespeichert in:
| Veröffentlicht in: | Acta biomaterialia 2007-07, Vol.3 (4), p.503-514 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 514 |
|---|---|
| container_issue | 4 |
| container_start_page | 503 |
| container_title | Acta biomaterialia |
| container_volume | 3 |
| creator | Abdel-Fattah, Wafa I. Jiang, Tao El-Bassyouni, Gehan El-Tabie Laurencin, Cato T. |
| description | The objective of the present study was to synthesize and characterize chitosans with different degrees of deacetylation (DDA%), prepare chitosan microspheres with controlled chemistry and geometry, and fabricate three-dimensional (3-D) chitosan matrices based on microspheres with appropriate pore size, porosity and mechanical properties suitable for bone tissue engineering applications. Chitosans with three DDA% of 69%, 79% and 97% were obtained using a thermomechanochemical technique by varying the applied pressure and NaOH solution concentration. The prepared chitosans were comprehensively characterized by proton nuclear magnetic resonance, elemental analysis, viscosity measurements, thermal analyses and X-ray diffraction. In addition, chitosan microspheres were prepared using an ionotropic gelation method. Three-dimensional chitosan matrices were fabricated via a sintered microsphere technique. Scanning electron microscopy revealed rough surfaces of the prepared chitosan microspheres. Mercury intrusion porosimetry revealed a porosity of 19.2% and a median pore diameter of 199.62
μm of the fabricated 3-D matrix. The compressive modulus of the sintered microsphere matrix (662.26
±
54.53
MPa) was in the range of human cancellous bone (10–2000
MPa), making it suitable for bone tissue engineering applications. |
| doi_str_mv | 10.1016/j.actbio.2006.12.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70601962</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1742706106001589</els_id><sourcerecordid>70601962</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-30491472633020a85cec95bae6750474019ef19ba9636e4b886dea38eb36f453</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo7rr6D0Ry8mS3lY9O0hdBFr9gwYN7D-l09U6G6WRMMsJ68LebYYb15p4Sqp56q3hfQl4z6Bkw9X7bO1-nkHoOoHrGewD5hFwyo02nB2Wetr-WvNOg2AV5UcoWQBjGzXNywbTgIEdxSf78uI91gyWUd9RvXG6amMNvV0OKNC2tFmoqLhbq4kwXN-XgH5olxEbj_EDRNficyn7TqnR1tcFY6JIynVJEWkMpB6QY70LEtibevSTPFrcr-Or8XpHbz59ur792N9-_fLv-eNN5yXntRDuWSc2VEMDBmcGjH4fJodIDSC2BjbiwcXKjEgrlZIya0QmDk1CLHMQVeXuS3ef084Cl2jUUj7udi5gOxTaPmoTij4ICoN0hHwc58HHUUjZQnsCjMSXjYvc5rC7fWwb2GKTd2lOQ9hikZdy2INvYm7P-YVpx_jd0Tq4BH04ANtt-Bcy2-IDR4xwy-mrnFP6_4S_yYLKX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20299744</pqid></control><display><type>article</type><title>Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Abdel-Fattah, Wafa I. ; Jiang, Tao ; El-Bassyouni, Gehan El-Tabie ; Laurencin, Cato T.</creator><creatorcontrib>Abdel-Fattah, Wafa I. ; Jiang, Tao ; El-Bassyouni, Gehan El-Tabie ; Laurencin, Cato T.</creatorcontrib><description>The objective of the present study was to synthesize and characterize chitosans with different degrees of deacetylation (DDA%), prepare chitosan microspheres with controlled chemistry and geometry, and fabricate three-dimensional (3-D) chitosan matrices based on microspheres with appropriate pore size, porosity and mechanical properties suitable for bone tissue engineering applications. Chitosans with three DDA% of 69%, 79% and 97% were obtained using a thermomechanochemical technique by varying the applied pressure and NaOH solution concentration. The prepared chitosans were comprehensively characterized by proton nuclear magnetic resonance, elemental analysis, viscosity measurements, thermal analyses and X-ray diffraction. In addition, chitosan microspheres were prepared using an ionotropic gelation method. Three-dimensional chitosan matrices were fabricated via a sintered microsphere technique. Scanning electron microscopy revealed rough surfaces of the prepared chitosan microspheres. Mercury intrusion porosimetry revealed a porosity of 19.2% and a median pore diameter of 199.62
μm of the fabricated 3-D matrix. The compressive modulus of the sintered microsphere matrix (662.26
±
54.53
MPa) was in the range of human cancellous bone (10–2000
MPa), making it suitable for bone tissue engineering applications.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2006.12.004</identifier><identifier>PMID: 17320493</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>3-D matrices ; Biocompatible Materials - metabolism ; Bone and Bones - metabolism ; Bone tissue engineering ; Chitin deacetylation ; Chitosan ; Chitosan - chemical synthesis ; Chitosan - chemistry ; Compressive Strength ; Electron Probe Microanalysis ; Magnetic Resonance Spectroscopy ; Materials Testing ; Microscopy, Electron, Scanning ; Microspheres ; Porosity ; Sodium Hydroxide - pharmacology ; Spectroscopy, Fourier Transform Infrared ; Thermogravimetry ; Tissue Engineering - methods ; Viscosity ; X-Ray Diffraction</subject><ispartof>Acta biomaterialia, 2007-07, Vol.3 (4), p.503-514</ispartof><rights>2007 Acta Materialia Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-30491472633020a85cec95bae6750474019ef19ba9636e4b886dea38eb36f453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actbio.2006.12.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17320493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdel-Fattah, Wafa I.</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>El-Bassyouni, Gehan El-Tabie</creatorcontrib><creatorcontrib>Laurencin, Cato T.</creatorcontrib><title>Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>The objective of the present study was to synthesize and characterize chitosans with different degrees of deacetylation (DDA%), prepare chitosan microspheres with controlled chemistry and geometry, and fabricate three-dimensional (3-D) chitosan matrices based on microspheres with appropriate pore size, porosity and mechanical properties suitable for bone tissue engineering applications. Chitosans with three DDA% of 69%, 79% and 97% were obtained using a thermomechanochemical technique by varying the applied pressure and NaOH solution concentration. The prepared chitosans were comprehensively characterized by proton nuclear magnetic resonance, elemental analysis, viscosity measurements, thermal analyses and X-ray diffraction. In addition, chitosan microspheres were prepared using an ionotropic gelation method. Three-dimensional chitosan matrices were fabricated via a sintered microsphere technique. Scanning electron microscopy revealed rough surfaces of the prepared chitosan microspheres. Mercury intrusion porosimetry revealed a porosity of 19.2% and a median pore diameter of 199.62
μm of the fabricated 3-D matrix. The compressive modulus of the sintered microsphere matrix (662.26
±
54.53
MPa) was in the range of human cancellous bone (10–2000
MPa), making it suitable for bone tissue engineering applications.</description><subject>3-D matrices</subject><subject>Biocompatible Materials - metabolism</subject><subject>Bone and Bones - metabolism</subject><subject>Bone tissue engineering</subject><subject>Chitin deacetylation</subject><subject>Chitosan</subject><subject>Chitosan - chemical synthesis</subject><subject>Chitosan - chemistry</subject><subject>Compressive Strength</subject><subject>Electron Probe Microanalysis</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microspheres</subject><subject>Porosity</subject><subject>Sodium Hydroxide - pharmacology</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Thermogravimetry</subject><subject>Tissue Engineering - methods</subject><subject>Viscosity</subject><subject>X-Ray Diffraction</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rr6D0Ry8mS3lY9O0hdBFr9gwYN7D-l09U6G6WRMMsJ68LebYYb15p4Sqp56q3hfQl4z6Bkw9X7bO1-nkHoOoHrGewD5hFwyo02nB2Wetr-WvNOg2AV5UcoWQBjGzXNywbTgIEdxSf78uI91gyWUd9RvXG6amMNvV0OKNC2tFmoqLhbq4kwXN-XgH5olxEbj_EDRNficyn7TqnR1tcFY6JIynVJEWkMpB6QY70LEtibevSTPFrcr-Or8XpHbz59ur792N9-_fLv-eNN5yXntRDuWSc2VEMDBmcGjH4fJodIDSC2BjbiwcXKjEgrlZIya0QmDk1CLHMQVeXuS3ef084Cl2jUUj7udi5gOxTaPmoTij4ICoN0hHwc58HHUUjZQnsCjMSXjYvc5rC7fWwb2GKTd2lOQ9hikZdy2INvYm7P-YVpx_jd0Tq4BH04ANtt-Bcy2-IDR4xwy-mrnFP6_4S_yYLKX</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Abdel-Fattah, Wafa I.</creator><creator>Jiang, Tao</creator><creator>El-Bassyouni, Gehan El-Tabie</creator><creator>Laurencin, Cato T.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering</title><author>Abdel-Fattah, Wafa I. ; Jiang, Tao ; El-Bassyouni, Gehan El-Tabie ; Laurencin, Cato T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-30491472633020a85cec95bae6750474019ef19ba9636e4b886dea38eb36f453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>3-D matrices</topic><topic>Biocompatible Materials - metabolism</topic><topic>Bone and Bones - metabolism</topic><topic>Bone tissue engineering</topic><topic>Chitin deacetylation</topic><topic>Chitosan</topic><topic>Chitosan - chemical synthesis</topic><topic>Chitosan - chemistry</topic><topic>Compressive Strength</topic><topic>Electron Probe Microanalysis</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Materials Testing</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microspheres</topic><topic>Porosity</topic><topic>Sodium Hydroxide - pharmacology</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Thermogravimetry</topic><topic>Tissue Engineering - methods</topic><topic>Viscosity</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdel-Fattah, Wafa I.</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>El-Bassyouni, Gehan El-Tabie</creatorcontrib><creatorcontrib>Laurencin, Cato T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biomaterialia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdel-Fattah, Wafa I.</au><au>Jiang, Tao</au><au>El-Bassyouni, Gehan El-Tabie</au><au>Laurencin, Cato T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering</atitle><jtitle>Acta biomaterialia</jtitle><addtitle>Acta Biomater</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>3</volume><issue>4</issue><spage>503</spage><epage>514</epage><pages>503-514</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>The objective of the present study was to synthesize and characterize chitosans with different degrees of deacetylation (DDA%), prepare chitosan microspheres with controlled chemistry and geometry, and fabricate three-dimensional (3-D) chitosan matrices based on microspheres with appropriate pore size, porosity and mechanical properties suitable for bone tissue engineering applications. Chitosans with three DDA% of 69%, 79% and 97% were obtained using a thermomechanochemical technique by varying the applied pressure and NaOH solution concentration. The prepared chitosans were comprehensively characterized by proton nuclear magnetic resonance, elemental analysis, viscosity measurements, thermal analyses and X-ray diffraction. In addition, chitosan microspheres were prepared using an ionotropic gelation method. Three-dimensional chitosan matrices were fabricated via a sintered microsphere technique. Scanning electron microscopy revealed rough surfaces of the prepared chitosan microspheres. Mercury intrusion porosimetry revealed a porosity of 19.2% and a median pore diameter of 199.62
μm of the fabricated 3-D matrix. The compressive modulus of the sintered microsphere matrix (662.26
±
54.53
MPa) was in the range of human cancellous bone (10–2000
MPa), making it suitable for bone tissue engineering applications.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17320493</pmid><doi>10.1016/j.actbio.2006.12.004</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1742-7061 |
| ispartof | Acta biomaterialia, 2007-07, Vol.3 (4), p.503-514 |
| issn | 1742-7061 1878-7568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70601962 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 3-D matrices Biocompatible Materials - metabolism Bone and Bones - metabolism Bone tissue engineering Chitin deacetylation Chitosan Chitosan - chemical synthesis Chitosan - chemistry Compressive Strength Electron Probe Microanalysis Magnetic Resonance Spectroscopy Materials Testing Microscopy, Electron, Scanning Microspheres Porosity Sodium Hydroxide - pharmacology Spectroscopy, Fourier Transform Infrared Thermogravimetry Tissue Engineering - methods Viscosity X-Ray Diffraction |
| title | Synthesis, characterization of chitosans and fabrication of sintered chitosan microsphere matrices for bone tissue engineering |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T18%3A49%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synthesis,%20characterization%20of%20chitosans%20and%20fabrication%20of%20sintered%20chitosan%20microsphere%20matrices%20for%20bone%20tissue%20engineering&rft.jtitle=Acta%20biomaterialia&rft.au=Abdel-Fattah,%20Wafa%20I.&rft.date=2007-07-01&rft.volume=3&rft.issue=4&rft.spage=503&rft.epage=514&rft.pages=503-514&rft.issn=1742-7061&rft.eissn=1878-7568&rft_id=info:doi/10.1016/j.actbio.2006.12.004&rft_dat=%3Cproquest_cross%3E70601962%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20299744&rft_id=info:pmid/17320493&rft_els_id=S1742706106001589&rfr_iscdi=true |