Microarray Analysis of the Developing Rat Mandible

To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1‐day‐old, 1‐week‐old, and 2‐week‐old rats, Complementa...

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Veröffentlicht in:	Acta biochimica et biophysica Sinica 2007-06, Vol.39 (6), p.399-405
Hauptverfasser:	KABURAGI, Hideo, SUGANO, Naoyuki, OSHIKAWA, Maiko, KOSHI, Ryosuke, SENDA, Naoki, KAWAMOTO, Kazuhiro, ITO, Koichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals DNA, Complementary - genetics Down-Regulation - physiology gene expression Gene Expression Profiling Mandible - growth & development microarray Microarray Analysis - methods Nucleic Acid Hybridization rat mandible Rats Reverse Transcriptase Polymerase Chain Reaction RNA - isolation & purification RNA, Complementary - genetics Up-Regulation - physiology
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Zusammenfassung:	To analyze the molecular events that occur in the developing mandible, we examined the expression of 8803 genes from samples taken at different time points during rat postnatal mandible development. Total RNA was extracted from the mandibles of 1‐day‐old, 1‐week‐old, and 2‐week‐old rats, Complementary RNA (cRNA) was synthesized from cDNA and biotinylated. Fragmented cRNA was hybridized to RGU34A GeneChip arrays. Among the 8803 genes tested, 4344 were detectable. We identified 148 genes with significantly increased expressions, and 19 genes with significantly decreased expression. A Comprehensive analysis appears to be an effective method of studying the complex process of development.
ISSN:	1672-9145 1745-7270
DOI:	10.1111/j.1745-7270.2007.00297.x