Enhancement of growth and coumarin production in hairy root cultures of witloof chicory ( Cichorium intybus L.cv.Lucknow local) under the influence of fungal elicitors
Studies were conducted to determine the possible production of coumarins in hairy root cultures of Cichorium intybus L.cv.Lucknow local under the influence of microbial agents. Pythium aphanidermatum and Phytopthora parasitica var. nicotiana were cultured and their mycelial and medium filtrate were...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2000, Vol.90 (6), p.648-653 |
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| creator | Bais, Harsh Pal Govindaswamy, Sudha Ravishankar, Gokare Aswathnarayana |
| description | Studies were conducted to determine the possible production of coumarins in hairy root cultures of
Cichorium intybus L.cv.Lucknow local under the influence of microbial agents.
Pythium aphanidermatum and
Phytopthora parasitica var.
nicotiana were cultured and their mycelial and medium filtrate were used for the elicitation of coumarin production. The media filtrate of
P. parasitica at 1.0% v/v added to the MS basal medium led to the maximum growth of hairy roots of
C. intybus, which was 1.57-fold higher than the untreated control on the 28th day of culture, along with maximum esculin and esculetin yields which were 4.06- and 3.71-fold higher than the control on the 28th day. The yields of esculin and esculetin in hairy root cultures of chicory strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine metabolism, titers of total endogenous polyamines were analyzed. It was confirmed that the media filtrate of
P. parasitica at 1.0% v/v concentration resulted in maximum accumulation of total endogenous polyamines, wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and putrescine titers on the 28th day. Total endogenous spermine in the case of 1.0% MF v/v
P. parasitica was 1.3-fold higher than that of the control on the 28th day of culture. Maximum growth index with greater length of primary root (17.61±0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium filtrate of
P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphological changes in terms of branching patterns, occurring in roots under the influence of these fungal elicitors. |
| doi_str_mv | 10.1016/S1389-1723(00)90011-2 |
| format | Article |
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Cichorium intybus L.cv.Lucknow local under the influence of microbial agents.
Pythium aphanidermatum and
Phytopthora parasitica var.
nicotiana were cultured and their mycelial and medium filtrate were used for the elicitation of coumarin production. The media filtrate of
P. parasitica at 1.0% v/v added to the MS basal medium led to the maximum growth of hairy roots of
C. intybus, which was 1.57-fold higher than the untreated control on the 28th day of culture, along with maximum esculin and esculetin yields which were 4.06- and 3.71-fold higher than the control on the 28th day. The yields of esculin and esculetin in hairy root cultures of chicory strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine metabolism, titers of total endogenous polyamines were analyzed. It was confirmed that the media filtrate of
P. parasitica at 1.0% v/v concentration resulted in maximum accumulation of total endogenous polyamines, wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and putrescine titers on the 28th day. Total endogenous spermine in the case of 1.0% MF v/v
P. parasitica was 1.3-fold higher than that of the control on the 28th day of culture. Maximum growth index with greater length of primary root (17.61±0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium filtrate of
P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphological changes in terms of branching patterns, occurring in roots under the influence of these fungal elicitors.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/S1389-1723(00)90011-2</identifier><identifier>PMID: 16232926</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biotechnology ; CICHORIUM INTYBUS ; Cichorium intybus L.cv.Lucknow local ; COUMARINS ; elicitor ; Establishment of new cell lines, improvement of cultural methods, mass culture ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; FUNGI ; GROWTH ; hairy root culture ; Methods. Procedures. Technologies ; Plant cells and fungal cells ; polyamines ; ROOTS ; TISSUE CULTURE</subject><ispartof>Journal of bioscience and bioengineering, 2000, Vol.90 (6), p.648-653</ispartof><rights>2000</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-2791fc6d8dbb6eb1c37f22bcf1dc142daf2ee9f0016ae8245fbbf72a9eb30f2d3</citedby><cites>FETCH-LOGICAL-c448t-2791fc6d8dbb6eb1c37f22bcf1dc142daf2ee9f0016ae8245fbbf72a9eb30f2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1389-1723(00)90011-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27921,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=870023$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16232926$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bais, Harsh Pal</creatorcontrib><creatorcontrib>Govindaswamy, Sudha</creatorcontrib><creatorcontrib>Ravishankar, Gokare Aswathnarayana</creatorcontrib><title>Enhancement of growth and coumarin production in hairy root cultures of witloof chicory ( Cichorium intybus L.cv.Lucknow local) under the influence of fungal elicitors</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Studies were conducted to determine the possible production of coumarins in hairy root cultures of
Cichorium intybus L.cv.Lucknow local under the influence of microbial agents.
Pythium aphanidermatum and
Phytopthora parasitica var.
nicotiana were cultured and their mycelial and medium filtrate were used for the elicitation of coumarin production. The media filtrate of
P. parasitica at 1.0% v/v added to the MS basal medium led to the maximum growth of hairy roots of
C. intybus, which was 1.57-fold higher than the untreated control on the 28th day of culture, along with maximum esculin and esculetin yields which were 4.06- and 3.71-fold higher than the control on the 28th day. The yields of esculin and esculetin in hairy root cultures of chicory strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine metabolism, titers of total endogenous polyamines were analyzed. It was confirmed that the media filtrate of
P. parasitica at 1.0% v/v concentration resulted in maximum accumulation of total endogenous polyamines, wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and putrescine titers on the 28th day. Total endogenous spermine in the case of 1.0% MF v/v
P. parasitica was 1.3-fold higher than that of the control on the 28th day of culture. Maximum growth index with greater length of primary root (17.61±0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium filtrate of
P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphological changes in terms of branching patterns, occurring in roots under the influence of these fungal elicitors.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CICHORIUM INTYBUS</subject><subject>Cichorium intybus L.cv.Lucknow local</subject><subject>COUMARINS</subject><subject>elicitor</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass culture</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGI</subject><subject>GROWTH</subject><subject>hairy root culture</subject><subject>Methods. Procedures. Technologies</subject><subject>Plant cells and fungal cells</subject><subject>polyamines</subject><subject>ROOTS</subject><subject>TISSUE CULTURE</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkduKFDEQhhtR3HX1EVYCguxe9JhDTx-uRIb1xICCeh3SSWU6mknWHHaYJ_I1Te80erlXSajvr6r8f1VdErwimLRvvhHWDzXpKLvC-HrAmJCaPqrOCWu6umkoeTzfF-Ssehbjz8J0uCNPqzPSUkYH2p5Xf27cJJyEPbiEvEa74A9pQsIpJH3ei2Acug1eZZmMd6i8JmHCEQXvE5LZphwgzsKDSdaXU05G-gJcoY2Rkw8m74sqHccc0XYl71bbLH85f0DWS2GvUXYKAkoTFErbDGWXuZ3ObicsAmukST7E59UTLWyEF8t5Uf14f_N987HefvnwafNuW8um6VNNu4Fo2apejWMLI5Gs05SOUhMlSUOV0BRg0MWJVkBPm7UeR91RMcDIsKaKXVSvT33Lp39niInvTZRgrXDgc-QdbjFum_5BkPTNUOweCrg-gTL4GANofhtMMfbICeZzlPw-Sj7nxDHm91FyWnQvlwF53IP6r1qyK8CrBRCxWKlDydHEf1zfYUxZoS5PlBaei10oxOevtMzAmK3ZXH97qkNx9c5A4FGaOQVlAsjElTcPLPoXq1HHVg</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Bais, Harsh Pal</creator><creator>Govindaswamy, Sudha</creator><creator>Ravishankar, Gokare Aswathnarayana</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Enhancement of growth and coumarin production in hairy root cultures of witloof chicory ( Cichorium intybus L.cv.Lucknow local) under the influence of fungal elicitors</title><author>Bais, Harsh Pal ; Govindaswamy, Sudha ; Ravishankar, Gokare Aswathnarayana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-2791fc6d8dbb6eb1c37f22bcf1dc142daf2ee9f0016ae8245fbbf72a9eb30f2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CICHORIUM INTYBUS</topic><topic>Cichorium intybus L.cv.Lucknow local</topic><topic>COUMARINS</topic><topic>elicitor</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass culture</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGI</topic><topic>GROWTH</topic><topic>hairy root culture</topic><topic>Methods. Procedures. Technologies</topic><topic>Plant cells and fungal cells</topic><topic>polyamines</topic><topic>ROOTS</topic><topic>TISSUE CULTURE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bais, Harsh Pal</creatorcontrib><creatorcontrib>Govindaswamy, Sudha</creatorcontrib><creatorcontrib>Ravishankar, Gokare Aswathnarayana</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bais, Harsh Pal</au><au>Govindaswamy, Sudha</au><au>Ravishankar, Gokare Aswathnarayana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of growth and coumarin production in hairy root cultures of witloof chicory ( Cichorium intybus L.cv.Lucknow local) under the influence of fungal elicitors</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2000</date><risdate>2000</risdate><volume>90</volume><issue>6</issue><spage>648</spage><epage>653</epage><pages>648-653</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Studies were conducted to determine the possible production of coumarins in hairy root cultures of
Cichorium intybus L.cv.Lucknow local under the influence of microbial agents.
Pythium aphanidermatum and
Phytopthora parasitica var.
nicotiana were cultured and their mycelial and medium filtrate were used for the elicitation of coumarin production. The media filtrate of
P. parasitica at 1.0% v/v added to the MS basal medium led to the maximum growth of hairy roots of
C. intybus, which was 1.57-fold higher than the untreated control on the 28th day of culture, along with maximum esculin and esculetin yields which were 4.06- and 3.71-fold higher than the control on the 28th day. The yields of esculin and esculetin in hairy root cultures of chicory strongly correlated with growth. To check the effect of these fungal elicitors on endogenous polyamine metabolism, titers of total endogenous polyamines were analyzed. It was confirmed that the media filtrate of
P. parasitica at 1.0% v/v concentration resulted in maximum accumulation of total endogenous polyamines, wherein endogenous spermine titers were found to be maximum as compared to endogenous spermidine and putrescine titers on the 28th day. Total endogenous spermine in the case of 1.0% MF v/v
P. parasitica was 1.3-fold higher than that of the control on the 28th day of culture. Maximum growth index with greater length of primary root (17.61±0.18 cm) and greater number of secondary and tertiary roots was recorded for the medium filtrate of
P. parasitica at 1.0% v/v concentration. This study also provided an insight into the morphological changes in terms of branching patterns, occurring in roots under the influence of these fungal elicitors.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>16232926</pmid><doi>10.1016/S1389-1723(00)90011-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2000, Vol.90 (6), p.648-653 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | Biological and medical sciences Biotechnology CICHORIUM INTYBUS Cichorium intybus L.cv.Lucknow local COUMARINS elicitor Establishment of new cell lines, improvement of cultural methods, mass culture Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology FUNGI GROWTH hairy root culture Methods. Procedures. Technologies Plant cells and fungal cells polyamines ROOTS TISSUE CULTURE |
| title | Enhancement of growth and coumarin production in hairy root cultures of witloof chicory ( Cichorium intybus L.cv.Lucknow local) under the influence of fungal elicitors |
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