Immunological identification of the mammalian H3 histamine receptor in the mouse brain
Affinity-purified antibodies raised against the peptide sequence H3 (349–358) receptor specifically recognized two protein species with Mr 62 000 and 93 000 in adult mouse forebrain membranes. Both immunoreactive species were suppressed greatly by preincubation of the antibody with the respective pe...
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| Veröffentlicht in: | Neuroreport 2001-02, Vol.12 (2), p.259-262 |
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| creator | Chazot, Paul L Hann, Victoria Wilson, Christopher Lees, George Thompson, Christopher L |
| description | Affinity-purified antibodies raised against the peptide sequence H3 (349–358) receptor specifically recognized two protein species with Mr 62 000 and 93 000 in adult mouse forebrain membranes. Both immunoreactive species were suppressed greatly by preincubation of the antibody with the respective peptide. Immunohistochemical analysis using affinity-purified anti-H3 (349–358) antibodies yielded a high degree of coincidence with ligand-autoradiographical information, with high levels detected in the CA3 and dentate gyrus of the hippocampus, laminae V of the cerebral cortex, the olfactory tubercle, Purkinje cell layer of the cerebellum, substantia nigra, globus pallidus, thalamus and striatum. This study suggests further biochemical evidence for multiple H3 receptor subtypes and the widespread distribution of the H3 receptor in the mammalian brain. |
| doi_str_mv | 10.1097/00001756-200102120-00016 |
| format | Article |
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Neuromudulation. Pathways and receptors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Peptide Fragments - immunology</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Histamine H3 - analysis</topic><topic>Receptors, Histamine H3 - immunology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chazot, Paul L</creatorcontrib><creatorcontrib>Hann, Victoria</creatorcontrib><creatorcontrib>Wilson, Christopher</creatorcontrib><creatorcontrib>Lees, George</creatorcontrib><creatorcontrib>Thompson, Christopher L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chazot, Paul L</au><au>Hann, Victoria</au><au>Wilson, Christopher</au><au>Lees, George</au><au>Thompson, Christopher L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological identification of the mammalian H3 histamine receptor in the mouse brain</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2001-02-12</date><risdate>2001</risdate><volume>12</volume><issue>2</issue><spage>259</spage><epage>262</epage><pages>259-262</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>Affinity-purified antibodies raised against the peptide sequence H3 (349–358) receptor specifically recognized two protein species with Mr 62 000 and 93 000 in adult mouse forebrain membranes. 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| ispartof | Neuroreport, 2001-02, Vol.12 (2), p.259-262 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Age Factors Animals Antibodies - isolation & purification Antibody Specificity Biological and medical sciences Brain Chemistry Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Fundamental and applied biological sciences. Psychology Humans Immunohistochemistry Mice Mice, Inbred Strains Peptide Fragments - immunology Rabbits Rats Rats, Wistar Receptors, Histamine H3 - analysis Receptors, Histamine H3 - immunology Vertebrates: nervous system and sense organs |
| title | Immunological identification of the mammalian H3 histamine receptor in the mouse brain |
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