Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure
A new biocompatible glass, which is composed of CaO, P2O5, SiO2, and Al2O3 (abbreviated CPSA) and is characterized by higher elasticity than previous bioglass products, was molded into fibers with a diameter of 9 μm. With CPSA fibers, two geometrically different structures, balls and bundles (each 2...
Gespeichert in:
| Veröffentlicht in: | Journal of biochemistry (Tokyo) 2001-01, Vol.129 (1), p.163-171 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 171 |
|---|---|
| container_issue | 1 |
| container_start_page | 163 |
| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 129 |
| creator | Mahmood, Javed Takita, Hiroko Ojima, Yasutaka Kobayashi, Masahiro Kohgo, Takao Kuboki, Yoshinori |
| description | A new biocompatible glass, which is composed of CaO, P2O5, SiO2, and Al2O3 (abbreviated CPSA) and is characterized by higher elasticity than previous bioglass products, was molded into fibers with a diameter of 9 μm. With CPSA fibers, two geometrically different structures, balls and bundles (each 20 mg in weight), were prepared, combined with 2.2 μg of rhBMP-2 (a gift from Yamanouchi Co., Japan) and implanted subcutaneously into rats. The histology showed remarkably higher bone formation in the ball-CPSA/ BMP at 2 and 4 weeks than in the bundle-CPSA/BMP. The ball-CPSA/BMP showed 10 times higher alkaline phosphatase (ALP) activity at the second week and 5 times higher osteocalcin content at the fourth week than the bundle-CSPA/BMP. Vascular development in the implants was evaluated by mRNA expression of Flt-1 and KDR, two receptors for vascular endothelial growth factor (VEGF). Both receptors showed higher expression in the case of the ball, while they were not detected in the bundle. It is concluded that the BMP-induced bone formation depends highly upon the porous vasculature-inducing geometry of the matrix, which can be constructed with the new CPSA fibers. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a002828 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70599518</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70599518</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-2bea194806b74e80e9f5d2b94a847e4db606566f0332abb4f04c4c5250b732a73</originalsourceid><addsrcrecordid>eNpVkEtvEzEUhb0A0VL6F5A3sJtgjz0PI7Gg6ROltOpDqrqxbM916jAzDrZHDQv-O64SgVj5-pxzr30_hD5QMqNEsE9-Y33oVn4Ko-rjbKXNEwwzRUjZlu0rtJ8LWoiSP-yhtzGuXq4lY2_QHqWUcdHQffT7DPwAKTiDT6wFk7C3-FJlYYOntR_xHPoeH7vsBRiTU8n58TM-urwuLsZuMtDhq5jAL2GE6CK-j25cYoW_wzM-cn7Zqxjxs0tPWTsFFZ3uAd-mMJk0BXiHXtv8czjcnQfo_vTkbn5eLK7OLuZfF4XhXKSi1KCo4C2pdcOhJSBs1ZVacNXyBnina1JXdW0JY6XSmlvCDTdVWRHdZKVhB-jjdu46-J8TxCQHF03eTI3gpygbUglR0TYHv2yDJvgYA1i5Dm5Q4ZekRL4wl_8zl1vmcsc897_fPTTpAbp_3TvgOVBsAy5T2_z1Vfgh64Y1lTx_eJR3j8c3C7H4JgX7AxdNl-c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70599518</pqid></control><display><type>article</type><title>Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure</title><source>J-STAGE Free</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Free Full-Text Journals in Chemistry</source><creator>Mahmood, Javed ; Takita, Hiroko ; Ojima, Yasutaka ; Kobayashi, Masahiro ; Kohgo, Takao ; Kuboki, Yoshinori</creator><creatorcontrib>Mahmood, Javed ; Takita, Hiroko ; Ojima, Yasutaka ; Kobayashi, Masahiro ; Kohgo, Takao ; Kuboki, Yoshinori</creatorcontrib><description>A new biocompatible glass, which is composed of CaO, P2O5, SiO2, and Al2O3 (abbreviated CPSA) and is characterized by higher elasticity than previous bioglass products, was molded into fibers with a diameter of 9 μm. With CPSA fibers, two geometrically different structures, balls and bundles (each 20 mg in weight), were prepared, combined with 2.2 μg of rhBMP-2 (a gift from Yamanouchi Co., Japan) and implanted subcutaneously into rats. The histology showed remarkably higher bone formation in the ball-CPSA/ BMP at 2 and 4 weeks than in the bundle-CPSA/BMP. The ball-CPSA/BMP showed 10 times higher alkaline phosphatase (ALP) activity at the second week and 5 times higher osteocalcin content at the fourth week than the bundle-CSPA/BMP. Vascular development in the implants was evaluated by mRNA expression of Flt-1 and KDR, two receptors for vascular endothelial growth factor (VEGF). Both receptors showed higher expression in the case of the ball, while they were not detected in the bundle. It is concluded that the BMP-induced bone formation depends highly upon the porous vasculature-inducing geometry of the matrix, which can be constructed with the new CPSA fibers.</description><identifier>ISSN: 0021-924X</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a002828</identifier><identifier>PMID: 11134971</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; bioglass ; Bone and Bones - physiology ; bone morphogenetic protein ; Bone Morphogenetic Proteins - physiology ; carrier-geometry ; Cell Differentiation - physiology ; Extracellular Matrix Proteins - biosynthesis ; Glass - chemistry ; Male ; Osteoblasts - metabolism ; Osteocalcin - genetics ; Osteocalcin - metabolism ; osteogenesis ; Osteogenesis - physiology ; Oxygen - metabolism ; Porosity ; Rats ; Rats, Wistar ; Receptor Protein-Tyrosine Kinases - biosynthesis ; Receptors, Growth Factor - biosynthesis ; Receptors, Vascular Endothelial Growth Factor ; RNA, Messenger - biosynthesis ; Vascular Endothelial Growth Factor Receptor-1 ; vascularization</subject><ispartof>Journal of biochemistry (Tokyo), 2001-01, Vol.129 (1), p.163-171</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-2bea194806b74e80e9f5d2b94a847e4db606566f0332abb4f04c4c5250b732a73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11134971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mahmood, Javed</creatorcontrib><creatorcontrib>Takita, Hiroko</creatorcontrib><creatorcontrib>Ojima, Yasutaka</creatorcontrib><creatorcontrib>Kobayashi, Masahiro</creatorcontrib><creatorcontrib>Kohgo, Takao</creatorcontrib><creatorcontrib>Kuboki, Yoshinori</creatorcontrib><title>Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>A new biocompatible glass, which is composed of CaO, P2O5, SiO2, and Al2O3 (abbreviated CPSA) and is characterized by higher elasticity than previous bioglass products, was molded into fibers with a diameter of 9 μm. With CPSA fibers, two geometrically different structures, balls and bundles (each 20 mg in weight), were prepared, combined with 2.2 μg of rhBMP-2 (a gift from Yamanouchi Co., Japan) and implanted subcutaneously into rats. The histology showed remarkably higher bone formation in the ball-CPSA/ BMP at 2 and 4 weeks than in the bundle-CPSA/BMP. The ball-CPSA/BMP showed 10 times higher alkaline phosphatase (ALP) activity at the second week and 5 times higher osteocalcin content at the fourth week than the bundle-CSPA/BMP. Vascular development in the implants was evaluated by mRNA expression of Flt-1 and KDR, two receptors for vascular endothelial growth factor (VEGF). Both receptors showed higher expression in the case of the ball, while they were not detected in the bundle. It is concluded that the BMP-induced bone formation depends highly upon the porous vasculature-inducing geometry of the matrix, which can be constructed with the new CPSA fibers.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>bioglass</subject><subject>Bone and Bones - physiology</subject><subject>bone morphogenetic protein</subject><subject>Bone Morphogenetic Proteins - physiology</subject><subject>carrier-geometry</subject><subject>Cell Differentiation - physiology</subject><subject>Extracellular Matrix Proteins - biosynthesis</subject><subject>Glass - chemistry</subject><subject>Male</subject><subject>Osteoblasts - metabolism</subject><subject>Osteocalcin - genetics</subject><subject>Osteocalcin - metabolism</subject><subject>osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>Oxygen - metabolism</subject><subject>Porosity</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor Protein-Tyrosine Kinases - biosynthesis</subject><subject>Receptors, Growth Factor - biosynthesis</subject><subject>Receptors, Vascular Endothelial Growth Factor</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Vascular Endothelial Growth Factor Receptor-1</subject><subject>vascularization</subject><issn>0021-924X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtvEzEUhb0A0VL6F5A3sJtgjz0PI7Gg6ROltOpDqrqxbM916jAzDrZHDQv-O64SgVj5-pxzr30_hD5QMqNEsE9-Y33oVn4Ko-rjbKXNEwwzRUjZlu0rtJ8LWoiSP-yhtzGuXq4lY2_QHqWUcdHQffT7DPwAKTiDT6wFk7C3-FJlYYOntR_xHPoeH7vsBRiTU8n58TM-urwuLsZuMtDhq5jAL2GE6CK-j25cYoW_wzM-cn7Zqxjxs0tPWTsFFZ3uAd-mMJk0BXiHXtv8czjcnQfo_vTkbn5eLK7OLuZfF4XhXKSi1KCo4C2pdcOhJSBs1ZVacNXyBnina1JXdW0JY6XSmlvCDTdVWRHdZKVhB-jjdu46-J8TxCQHF03eTI3gpygbUglR0TYHv2yDJvgYA1i5Dm5Q4ZekRL4wl_8zl1vmcsc897_fPTTpAbp_3TvgOVBsAy5T2_z1Vfgh64Y1lTx_eJR3j8c3C7H4JgX7AxdNl-c</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Mahmood, Javed</creator><creator>Takita, Hiroko</creator><creator>Ojima, Yasutaka</creator><creator>Kobayashi, Masahiro</creator><creator>Kohgo, Takao</creator><creator>Kuboki, Yoshinori</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure</title><author>Mahmood, Javed ; Takita, Hiroko ; Ojima, Yasutaka ; Kobayashi, Masahiro ; Kohgo, Takao ; Kuboki, Yoshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-2bea194806b74e80e9f5d2b94a847e4db606566f0332abb4f04c4c5250b732a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>bioglass</topic><topic>Bone and Bones - physiology</topic><topic>bone morphogenetic protein</topic><topic>Bone Morphogenetic Proteins - physiology</topic><topic>carrier-geometry</topic><topic>Cell Differentiation - physiology</topic><topic>Extracellular Matrix Proteins - biosynthesis</topic><topic>Glass - chemistry</topic><topic>Male</topic><topic>Osteoblasts - metabolism</topic><topic>Osteocalcin - genetics</topic><topic>Osteocalcin - metabolism</topic><topic>osteogenesis</topic><topic>Osteogenesis - physiology</topic><topic>Oxygen - metabolism</topic><topic>Porosity</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor Protein-Tyrosine Kinases - biosynthesis</topic><topic>Receptors, Growth Factor - biosynthesis</topic><topic>Receptors, Vascular Endothelial Growth Factor</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Vascular Endothelial Growth Factor Receptor-1</topic><topic>vascularization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahmood, Javed</creatorcontrib><creatorcontrib>Takita, Hiroko</creatorcontrib><creatorcontrib>Ojima, Yasutaka</creatorcontrib><creatorcontrib>Kobayashi, Masahiro</creatorcontrib><creatorcontrib>Kohgo, Takao</creatorcontrib><creatorcontrib>Kuboki, Yoshinori</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahmood, Javed</au><au>Takita, Hiroko</au><au>Ojima, Yasutaka</au><au>Kobayashi, Masahiro</au><au>Kohgo, Takao</au><au>Kuboki, Yoshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>2001-01</date><risdate>2001</risdate><volume>129</volume><issue>1</issue><spage>163</spage><epage>171</epage><pages>163-171</pages><issn>0021-924X</issn><abstract>A new biocompatible glass, which is composed of CaO, P2O5, SiO2, and Al2O3 (abbreviated CPSA) and is characterized by higher elasticity than previous bioglass products, was molded into fibers with a diameter of 9 μm. With CPSA fibers, two geometrically different structures, balls and bundles (each 20 mg in weight), were prepared, combined with 2.2 μg of rhBMP-2 (a gift from Yamanouchi Co., Japan) and implanted subcutaneously into rats. The histology showed remarkably higher bone formation in the ball-CPSA/ BMP at 2 and 4 weeks than in the bundle-CPSA/BMP. The ball-CPSA/BMP showed 10 times higher alkaline phosphatase (ALP) activity at the second week and 5 times higher osteocalcin content at the fourth week than the bundle-CSPA/BMP. Vascular development in the implants was evaluated by mRNA expression of Flt-1 and KDR, two receptors for vascular endothelial growth factor (VEGF). Both receptors showed higher expression in the case of the ball, while they were not detected in the bundle. It is concluded that the BMP-induced bone formation depends highly upon the porous vasculature-inducing geometry of the matrix, which can be constructed with the new CPSA fibers.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>11134971</pmid><doi>10.1093/oxfordjournals.jbchem.a002828</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-924X |
| ispartof | Journal of biochemistry (Tokyo), 2001-01, Vol.129 (1), p.163-171 |
| issn | 0021-924X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70599518 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Free Full-Text Journals in Chemistry |
| subjects | Alkaline Phosphatase - metabolism Animals bioglass Bone and Bones - physiology bone morphogenetic protein Bone Morphogenetic Proteins - physiology carrier-geometry Cell Differentiation - physiology Extracellular Matrix Proteins - biosynthesis Glass - chemistry Male Osteoblasts - metabolism Osteocalcin - genetics Osteocalcin - metabolism osteogenesis Osteogenesis - physiology Oxygen - metabolism Porosity Rats Rats, Wistar Receptor Protein-Tyrosine Kinases - biosynthesis Receptors, Growth Factor - biosynthesis Receptors, Vascular Endothelial Growth Factor RNA, Messenger - biosynthesis Vascular Endothelial Growth Factor Receptor-1 vascularization |
| title | Geometric Effect of Matrix upon Cell Differentiation: BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T13%3A13%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Geometric%20Effect%20of%20Matrix%20upon%20Cell%20Differentiation:%20BMP-Induced%20Osteogenesis%20Using%20a%20New%20Bioglass%20with%20a%20Feasible%20Structure&rft.jtitle=Journal%20of%20biochemistry%20(Tokyo)&rft.au=Mahmood,%20Javed&rft.date=2001-01&rft.volume=129&rft.issue=1&rft.spage=163&rft.epage=171&rft.pages=163-171&rft.issn=0021-924X&rft_id=info:doi/10.1093/oxfordjournals.jbchem.a002828&rft_dat=%3Cproquest_cross%3E70599518%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70599518&rft_id=info:pmid/11134971&rfr_iscdi=true |