Determination of Blomia tropicalis‐specific IgE and IgG subclasses in atopic dermatitis patients
Background: To verify the importance of Blomia tropicalis in atopic dermatitis (AD), we determined the cutaneous reactivity and the serum level of B. tropicalis‐specific IgE and IgG subclasses in AD patients. Methods: B. tropicalis‐specific IgE and IgG subclasses were determined in AD patients and c...
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| Veröffentlicht in: | Allergy (Copenhagen) 2001-02, Vol.56 (2), p.180-184 |
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| creator | Mori, J. C. Pires, M. C. Galvão, C. E. S. Ferreira de Mello, J. Golcher, F. M. Montealegre, F. |
| description | Background: To verify the importance of Blomia tropicalis in atopic dermatitis (AD), we determined the cutaneous reactivity and the serum level of B. tropicalis‐specific IgE and IgG subclasses in AD patients.
Methods: B. tropicalis‐specific IgE and IgG subclasses were determined in AD patients and compared with bronchial asthma (BA) patients and a control group (CG) of nonatopic subjects. Specific IgE was obtained by skin prick test and RAST. B. tropicalis‐specific IgG subclasses were determined by ELISA. The data were statistically analyzed by chi‐square test (Mantel‐Haenszel) and odds ratio (OR).
Results: We detected positive skin prick tests in 61.76% of AD and 83.33% of BA patients, and in 12.5% of the CG. RAST was positive in 44.12% of AD and in 61.90% of BA patients, but not in the CG. B. tropicalis‐specific IgG1 and IgG2 subclasses showed no significant differences between the three groups. IgG3 subclass positivity was statistically significant in AD patients (41.17%) when compared to BA patients (14.29%) and the CG (16.67%). The determination of B. tropicalis‐specific IgG4 was positive in 32.35% of AD patients, 21.43% of BA patients, and 8.33% of the CG.
Conclusions: These results confirm that the storage mite B. tropicalis is an important allergen in AD. It is possible that IgG3 activates the complement in AD patients, releasing vasoactive amines that further amplify the allergic reaction. The positive results of the B. tropicalis‐specific IgG4 found in AD and BA were probably due to chronic exposure to this storage mite in the home environment. |
| doi_str_mv | 10.1034/j.1398-9995.2001.056002180.x |
| format | Article |
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Methods: B. tropicalis‐specific IgE and IgG subclasses were determined in AD patients and compared with bronchial asthma (BA) patients and a control group (CG) of nonatopic subjects. Specific IgE was obtained by skin prick test and RAST. B. tropicalis‐specific IgG subclasses were determined by ELISA. The data were statistically analyzed by chi‐square test (Mantel‐Haenszel) and odds ratio (OR).
Results: We detected positive skin prick tests in 61.76% of AD and 83.33% of BA patients, and in 12.5% of the CG. RAST was positive in 44.12% of AD and in 61.90% of BA patients, but not in the CG. B. tropicalis‐specific IgG1 and IgG2 subclasses showed no significant differences between the three groups. IgG3 subclass positivity was statistically significant in AD patients (41.17%) when compared to BA patients (14.29%) and the CG (16.67%). The determination of B. tropicalis‐specific IgG4 was positive in 32.35% of AD patients, 21.43% of BA patients, and 8.33% of the CG.
Conclusions: These results confirm that the storage mite B. tropicalis is an important allergen in AD. It is possible that IgG3 activates the complement in AD patients, releasing vasoactive amines that further amplify the allergic reaction. The positive results of the B. tropicalis‐specific IgG4 found in AD and BA were probably due to chronic exposure to this storage mite in the home environment.</description><identifier>ISSN: 0105-4538</identifier><identifier>EISSN: 1398-9995</identifier><identifier>DOI: 10.1034/j.1398-9995.2001.056002180.x</identifier><identifier>PMID: 11167381</identifier><identifier>CODEN: LLRGDY</identifier><language>eng</language><publisher>Copenhagen: Munksgaard International Publishers</publisher><subject>Adolescent ; Adult ; Aged ; Allergic diseases ; Animals ; Asthma - immunology ; atopic dermatitis ; Biological and medical sciences ; Blomia tropicalis ; Child ; Child, Preschool ; Dermatitis, Contact - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin E - analysis ; Immunoglobulin E - classification ; Immunoglobulin G - analysis ; Immunoglobulin G - classification ; Immunopathology ; Infant ; Male ; Medical sciences ; Middle Aged ; Mites - immunology ; Radioallergosorbent Test ; Reference Values ; Skin allergic diseases. Stinging insect allergies ; Skin Tests ; storage mites</subject><ispartof>Allergy (Copenhagen), 2001-02, Vol.56 (2), p.180-184</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4359-b7c4d71c71030302632799cfa44121598d47bef821924f8d5350c788fee3950d3</citedby><cites>FETCH-LOGICAL-c4359-b7c4d71c71030302632799cfa44121598d47bef821924f8d5350c788fee3950d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1398-9995.2001.056002180.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1398-9995.2001.056002180.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=877570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11167381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mori, J. C.</creatorcontrib><creatorcontrib>Pires, M. C.</creatorcontrib><creatorcontrib>Galvão, C. E. S.</creatorcontrib><creatorcontrib>Ferreira de Mello, J.</creatorcontrib><creatorcontrib>Golcher, F. M.</creatorcontrib><creatorcontrib>Montealegre, F.</creatorcontrib><title>Determination of Blomia tropicalis‐specific IgE and IgG subclasses in atopic dermatitis patients</title><title>Allergy (Copenhagen)</title><addtitle>Allergy</addtitle><description>Background: To verify the importance of Blomia tropicalis in atopic dermatitis (AD), we determined the cutaneous reactivity and the serum level of B. tropicalis‐specific IgE and IgG subclasses in AD patients.
Methods: B. tropicalis‐specific IgE and IgG subclasses were determined in AD patients and compared with bronchial asthma (BA) patients and a control group (CG) of nonatopic subjects. Specific IgE was obtained by skin prick test and RAST. B. tropicalis‐specific IgG subclasses were determined by ELISA. The data were statistically analyzed by chi‐square test (Mantel‐Haenszel) and odds ratio (OR).
Results: We detected positive skin prick tests in 61.76% of AD and 83.33% of BA patients, and in 12.5% of the CG. RAST was positive in 44.12% of AD and in 61.90% of BA patients, but not in the CG. B. tropicalis‐specific IgG1 and IgG2 subclasses showed no significant differences between the three groups. IgG3 subclass positivity was statistically significant in AD patients (41.17%) when compared to BA patients (14.29%) and the CG (16.67%). The determination of B. tropicalis‐specific IgG4 was positive in 32.35% of AD patients, 21.43% of BA patients, and 8.33% of the CG.
Conclusions: These results confirm that the storage mite B. tropicalis is an important allergen in AD. It is possible that IgG3 activates the complement in AD patients, releasing vasoactive amines that further amplify the allergic reaction. The positive results of the B. tropicalis‐specific IgG4 found in AD and BA were probably due to chronic exposure to this storage mite in the home environment.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>Asthma - immunology</subject><subject>atopic dermatitis</subject><subject>Biological and medical sciences</subject><subject>Blomia tropicalis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Dermatitis, Contact - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin E - analysis</subject><subject>Immunoglobulin E - classification</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunoglobulin G - classification</subject><subject>Immunopathology</subject><subject>Infant</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mites - immunology</subject><subject>Radioallergosorbent Test</subject><subject>Reference Values</subject><subject>Skin allergic diseases. Stinging insect allergies</subject><subject>Skin Tests</subject><subject>storage mites</subject><issn>0105-4538</issn><issn>1398-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1DAUhS1ERYfCKyBLIHZJr__GtsSCUkpbaSQ2sLYcx0Ee5Y_cjNrueASesU-CoxlNl6jy4iz83ePrcwj5wKBkIOT5tmTCmsJaq0oOwEpQawDODJT3L8jqePmSrICBKqQS5pS8RtwCgOYWXpFTxthaC8NWpPoa5zh1qfdzGno6NPRLO3TJ03kaxhR8m_Dxz18cY0hNCvT21xX1fZ31muKuCq1HjEhTT_288LTOZtlqTkjHrLGf8Q05aXyL8e1Bz8jPb1c_Lm-Kzffr28uLTRGkULaodJC1ZkHnX-bD14Jra0PjpWScKWtqqavYGM4sl42plVAQtDFNjMIqqMUZ-bj3Hafh9y7i7LqEIbat7-OwQ6dBWSsV-y_ItJHKcp3BT3swTAPiFBs3Tqnz04Nj4JYy3NYtebslb7eU4Y5luPs8_u7wzq7qYv00fEg_A-8PgMccdTP5PiQ8ckZrpSFTn_fUXWrjw7NWcBebjQYr_gFNVqX2</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Mori, J. C.</creator><creator>Pires, M. C.</creator><creator>Galvão, C. E. S.</creator><creator>Ferreira de Mello, J.</creator><creator>Golcher, F. M.</creator><creator>Montealegre, F.</creator><general>Munksgaard International Publishers</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>Determination of Blomia tropicalis‐specific IgE and IgG subclasses in atopic dermatitis patients</title><author>Mori, J. C. ; Pires, M. C. ; Galvão, C. E. S. ; Ferreira de Mello, J. ; Golcher, F. M. ; Montealegre, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4359-b7c4d71c71030302632799cfa44121598d47bef821924f8d5350c788fee3950d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>Asthma - immunology</topic><topic>atopic dermatitis</topic><topic>Biological and medical sciences</topic><topic>Blomia tropicalis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Dermatitis, Contact - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin E - analysis</topic><topic>Immunoglobulin E - classification</topic><topic>Immunoglobulin G - analysis</topic><topic>Immunoglobulin G - classification</topic><topic>Immunopathology</topic><topic>Infant</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mites - immunology</topic><topic>Radioallergosorbent Test</topic><topic>Reference Values</topic><topic>Skin allergic diseases. Stinging insect allergies</topic><topic>Skin Tests</topic><topic>storage mites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mori, J. C.</creatorcontrib><creatorcontrib>Pires, M. C.</creatorcontrib><creatorcontrib>Galvão, C. E. S.</creatorcontrib><creatorcontrib>Ferreira de Mello, J.</creatorcontrib><creatorcontrib>Golcher, F. M.</creatorcontrib><creatorcontrib>Montealegre, F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Allergy (Copenhagen)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mori, J. C.</au><au>Pires, M. C.</au><au>Galvão, C. E. S.</au><au>Ferreira de Mello, J.</au><au>Golcher, F. M.</au><au>Montealegre, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Blomia tropicalis‐specific IgE and IgG subclasses in atopic dermatitis patients</atitle><jtitle>Allergy (Copenhagen)</jtitle><addtitle>Allergy</addtitle><date>2001-02</date><risdate>2001</risdate><volume>56</volume><issue>2</issue><spage>180</spage><epage>184</epage><pages>180-184</pages><issn>0105-4538</issn><eissn>1398-9995</eissn><coden>LLRGDY</coden><abstract>Background: To verify the importance of Blomia tropicalis in atopic dermatitis (AD), we determined the cutaneous reactivity and the serum level of B. tropicalis‐specific IgE and IgG subclasses in AD patients.
Methods: B. tropicalis‐specific IgE and IgG subclasses were determined in AD patients and compared with bronchial asthma (BA) patients and a control group (CG) of nonatopic subjects. Specific IgE was obtained by skin prick test and RAST. B. tropicalis‐specific IgG subclasses were determined by ELISA. The data were statistically analyzed by chi‐square test (Mantel‐Haenszel) and odds ratio (OR).
Results: We detected positive skin prick tests in 61.76% of AD and 83.33% of BA patients, and in 12.5% of the CG. RAST was positive in 44.12% of AD and in 61.90% of BA patients, but not in the CG. B. tropicalis‐specific IgG1 and IgG2 subclasses showed no significant differences between the three groups. IgG3 subclass positivity was statistically significant in AD patients (41.17%) when compared to BA patients (14.29%) and the CG (16.67%). The determination of B. tropicalis‐specific IgG4 was positive in 32.35% of AD patients, 21.43% of BA patients, and 8.33% of the CG.
Conclusions: These results confirm that the storage mite B. tropicalis is an important allergen in AD. It is possible that IgG3 activates the complement in AD patients, releasing vasoactive amines that further amplify the allergic reaction. The positive results of the B. tropicalis‐specific IgG4 found in AD and BA were probably due to chronic exposure to this storage mite in the home environment.</abstract><cop>Copenhagen</cop><pub>Munksgaard International Publishers</pub><pmid>11167381</pmid><doi>10.1034/j.1398-9995.2001.056002180.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Allergic diseases Animals Asthma - immunology atopic dermatitis Biological and medical sciences Blomia tropicalis Child Child, Preschool Dermatitis, Contact - immunology Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin E - analysis Immunoglobulin E - classification Immunoglobulin G - analysis Immunoglobulin G - classification Immunopathology Infant Male Medical sciences Middle Aged Mites - immunology Radioallergosorbent Test Reference Values Skin allergic diseases. Stinging insect allergies Skin Tests storage mites |
| title | Determination of Blomia tropicalis‐specific IgE and IgG subclasses in atopic dermatitis patients |
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