Improved high performance liquid chromatographic analysis of omeprazole in human plasma
A simple high-performance liquid chromatographic method was developed for the determination of omeprazole in human plasma. Omeprazole and the internal standard, chloramphenicol, were extracted from alkalinized plasma samples using dichloromethane. The mobile phase was 0.05 M Na 2HPO 4–ACN (65:35, v/...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2001-02, Vol.24 (4), p.715-719 |
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| container_end_page | 719 |
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| container_issue | 4 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 24 |
| creator | Yuen, Kah Hay Choy, Wai Peng Tan, Huey Yin Wong, Jia Woei Yap, Siew Ping |
| description | A simple high-performance liquid chromatographic method was developed for the determination of omeprazole in human plasma. Omeprazole and the internal standard, chloramphenicol, were extracted from alkalinized plasma samples using dichloromethane. The mobile phase was 0.05 M Na
2HPO
4–ACN (65:35, v/v) adjusted to pH 6.5. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 302 nm. The method was specific and sensitive with a detection limit of 2.5 ng/ml at a signal-to-noise ratio of 4:1. The limit of quantification was set at 5 ng/ml. The calibration curve was linear over a concentration range of 5–1280 ng/ml. Mean recovery value of the extraction procedure was about 96%, while the within and between day coefficient of variation and percent error values of the assay method were all less than 14%. |
| doi_str_mv | 10.1016/S0731-7085(00)00473-8 |
| format | Article |
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2HPO
4–ACN (65:35, v/v) adjusted to pH 6.5. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 302 nm. The method was specific and sensitive with a detection limit of 2.5 ng/ml at a signal-to-noise ratio of 4:1. The limit of quantification was set at 5 ng/ml. The calibration curve was linear over a concentration range of 5–1280 ng/ml. Mean recovery value of the extraction procedure was about 96%, while the within and between day coefficient of variation and percent error values of the assay method were all less than 14%.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/S0731-7085(00)00473-8</identifier><identifier>PMID: 11272330</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Biological and medical sciences ; Chloramphenicol - blood ; Chromatography, High Pressure Liquid - methods ; General pharmacology ; Humans ; Medical sciences ; Omeprazole ; Omeprazole - blood ; Pharmacology. Drug treatments ; Reversed phase HPLC assay ; Sensitivity and Specificity</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2001-02, Vol.24 (4), p.715-719</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-df0326bc1ac284b06e531c08af6c0a608fff9600dc6ac9ffda97b42ce5bbfff43</citedby><cites>FETCH-LOGICAL-c389t-df0326bc1ac284b06e531c08af6c0a608fff9600dc6ac9ffda97b42ce5bbfff43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708500004738$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=881780$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11272330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuen, Kah Hay</creatorcontrib><creatorcontrib>Choy, Wai Peng</creatorcontrib><creatorcontrib>Tan, Huey Yin</creatorcontrib><creatorcontrib>Wong, Jia Woei</creatorcontrib><creatorcontrib>Yap, Siew Ping</creatorcontrib><title>Improved high performance liquid chromatographic analysis of omeprazole in human plasma</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A simple high-performance liquid chromatographic method was developed for the determination of omeprazole in human plasma. Omeprazole and the internal standard, chloramphenicol, were extracted from alkalinized plasma samples using dichloromethane. The mobile phase was 0.05 M Na
2HPO
4–ACN (65:35, v/v) adjusted to pH 6.5. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 302 nm. The method was specific and sensitive with a detection limit of 2.5 ng/ml at a signal-to-noise ratio of 4:1. The limit of quantification was set at 5 ng/ml. The calibration curve was linear over a concentration range of 5–1280 ng/ml. Mean recovery value of the extraction procedure was about 96%, while the within and between day coefficient of variation and percent error values of the assay method were all less than 14%.</description><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chloramphenicol - blood</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Omeprazole</subject><subject>Omeprazole - blood</subject><subject>Pharmacology. Drug treatments</subject><subject>Reversed phase HPLC assay</subject><subject>Sensitivity and Specificity</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN9LHDEQx4MoerX-CUpAKO3D2sn-zD6VItUeCD7Y0r6F2ezEjexe9pJdQf96c96hjz5NYD7fmcyHsVMBFwJE-f0OqkwkFcjiK8A3gLzKErnHFkLGR1rm__fZ4g05Yp9CeACAQtT5ITsSIq3SLIMF-7ccRu8eqeWdve_4SN44P-BKE-_terYt1513A07u3uPYWc1xhf1TsIE7w91Ao8dn1xO3K97NMcjHHsOAn9mBwT7Qya4es79Xv_5c_k5ubq-Xlz9vEp3JekpaA1laNlqgTmXeQElFJjRINKUGLEEaY-oSoNUl6tqYFuuqyVNNRdPEVp4dsy_bufGK9UxhUoMNmvoeV-TmoCoo6ni1iGCxBbV3IXgyavR2QP-kBKiNUfVqVG10KQD1alTJmDvbLZibgdr31E5hBM53AAaNvfFRng1vnJSikhvqx5aiKOPRkldBW4qaW-tJT6p19oOPvACtuZSQ</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Yuen, Kah Hay</creator><creator>Choy, Wai Peng</creator><creator>Tan, Huey Yin</creator><creator>Wong, Jia Woei</creator><creator>Yap, Siew Ping</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Improved high performance liquid chromatographic analysis of omeprazole in human plasma</title><author>Yuen, Kah Hay ; Choy, Wai Peng ; Tan, Huey Yin ; Wong, Jia Woei ; Yap, Siew Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-df0326bc1ac284b06e531c08af6c0a608fff9600dc6ac9ffda97b42ce5bbfff43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Chloramphenicol - blood</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Omeprazole</topic><topic>Omeprazole - blood</topic><topic>Pharmacology. Drug treatments</topic><topic>Reversed phase HPLC assay</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuen, Kah Hay</creatorcontrib><creatorcontrib>Choy, Wai Peng</creatorcontrib><creatorcontrib>Tan, Huey Yin</creatorcontrib><creatorcontrib>Wong, Jia Woei</creatorcontrib><creatorcontrib>Yap, Siew Ping</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuen, Kah Hay</au><au>Choy, Wai Peng</au><au>Tan, Huey Yin</au><au>Wong, Jia Woei</au><au>Yap, Siew Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved high performance liquid chromatographic analysis of omeprazole in human plasma</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>24</volume><issue>4</issue><spage>715</spage><epage>719</epage><pages>715-719</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A simple high-performance liquid chromatographic method was developed for the determination of omeprazole in human plasma. Omeprazole and the internal standard, chloramphenicol, were extracted from alkalinized plasma samples using dichloromethane. The mobile phase was 0.05 M Na
2HPO
4–ACN (65:35, v/v) adjusted to pH 6.5. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 302 nm. The method was specific and sensitive with a detection limit of 2.5 ng/ml at a signal-to-noise ratio of 4:1. The limit of quantification was set at 5 ng/ml. The calibration curve was linear over a concentration range of 5–1280 ng/ml. Mean recovery value of the extraction procedure was about 96%, while the within and between day coefficient of variation and percent error values of the assay method were all less than 14%.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11272330</pmid><doi>10.1016/S0731-7085(00)00473-8</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical and biomedical analysis, 2001-02, Vol.24 (4), p.715-719 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Biological and medical sciences Chloramphenicol - blood Chromatography, High Pressure Liquid - methods General pharmacology Humans Medical sciences Omeprazole Omeprazole - blood Pharmacology. Drug treatments Reversed phase HPLC assay Sensitivity and Specificity |
| title | Improved high performance liquid chromatographic analysis of omeprazole in human plasma |
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