S-Adenosylmethionine reverses ilimaquinone's vesiculation of the Golgi apparatus: A fluorescence study on the cellular interactions of ilimaquinone
The marine sponge metabolite ilimaquinone has a wide range of biological activities, including vesiculation of the Golgi apparatus and interference with intracellular protein trafficking. Some of these activities may arise from ilimaquinone's influence on the activated methyl cycle. To visualiz...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2001-01, Vol.11 (2), p.133-136 |
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| creator | CASAUBON, Rebecca L SNAPPER, Marc L |
| description | The marine sponge metabolite ilimaquinone has a wide range of biological activities, including vesiculation of the Golgi apparatus and interference with intracellular protein trafficking. Some of these activities may arise from ilimaquinone's influence on the activated methyl cycle. To visualize the morphological effects of ilimaquinone on the Golgi apparatus, NRK (normal rat kidney) cells were labeled with fluorescent wheat germ agglutinin and treated with ilimaquinone in the presence and absence of the methylating agent S-adenosylmethionine (SAMe). While ilimaquinone alone fragments the Golgi apparatus, the organelle remains intact when SAMe is included in the incubation mixture. This observation supports ilimaquinone's interaction with methylation enzymes as the cause of Golgi vesiculation. The examination of a fluorescently labeled ilimaquinone analogue in NRK cells suggests that the cellular interactions of ilimaquinone are not localized to the Golgi apparatus. |
| doi_str_mv | 10.1016/S0960-894X(00)00617-X |
| format | Article |
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Some of these activities may arise from ilimaquinone's influence on the activated methyl cycle. To visualize the morphological effects of ilimaquinone on the Golgi apparatus, NRK (normal rat kidney) cells were labeled with fluorescent wheat germ agglutinin and treated with ilimaquinone in the presence and absence of the methylating agent S-adenosylmethionine (SAMe). While ilimaquinone alone fragments the Golgi apparatus, the organelle remains intact when SAMe is included in the incubation mixture. This observation supports ilimaquinone's interaction with methylation enzymes as the cause of Golgi vesiculation. The examination of a fluorescently labeled ilimaquinone analogue in NRK cells suggests that the cellular interactions of ilimaquinone are not localized to the Golgi apparatus.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/S0960-894X(00)00617-X</identifier><identifier>PMID: 11206443</identifier><language>eng</language><publisher>Oxford: Elsevier</publisher><subject>Adenosylhomocysteinase ; Animals ; Biological and medical sciences ; Cell structures and functions ; Cells, Cultured ; Cytoplasmic Vesicles - drug effects ; Cytoplasmic Vesicles - metabolism ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Golgi apparatus ; Golgi Apparatus - drug effects ; Hydrolases - metabolism ; Kidney - cytology ; Methyltransferases - metabolism ; Microscopy, Fluorescence ; Molecular and cellular biology ; Protein Transport - drug effects ; Quinones - chemistry ; Quinones - metabolism ; Quinones - pharmacology ; Rats ; S-Adenosylmethionine - metabolism ; S-Adenosylmethionine - pharmacology ; Structure-Activity Relationship</subject><ispartof>Bioorganic & medicinal chemistry letters, 2001-01, Vol.11 (2), p.133-136</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=871307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11206443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CASAUBON, Rebecca L</creatorcontrib><creatorcontrib>SNAPPER, Marc L</creatorcontrib><title>S-Adenosylmethionine reverses ilimaquinone's vesiculation of the Golgi apparatus: A fluorescence study on the cellular interactions of ilimaquinone</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>The marine sponge metabolite ilimaquinone has a wide range of biological activities, including vesiculation of the Golgi apparatus and interference with intracellular protein trafficking. Some of these activities may arise from ilimaquinone's influence on the activated methyl cycle. To visualize the morphological effects of ilimaquinone on the Golgi apparatus, NRK (normal rat kidney) cells were labeled with fluorescent wheat germ agglutinin and treated with ilimaquinone in the presence and absence of the methylating agent S-adenosylmethionine (SAMe). While ilimaquinone alone fragments the Golgi apparatus, the organelle remains intact when SAMe is included in the incubation mixture. This observation supports ilimaquinone's interaction with methylation enzymes as the cause of Golgi vesiculation. The examination of a fluorescently labeled ilimaquinone analogue in NRK cells suggests that the cellular interactions of ilimaquinone are not localized to the Golgi apparatus.</description><subject>Adenosylhomocysteinase</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Cells, Cultured</subject><subject>Cytoplasmic Vesicles - drug effects</subject><subject>Cytoplasmic Vesicles - metabolism</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Golgi apparatus</subject><subject>Golgi Apparatus - drug effects</subject><subject>Hydrolases - metabolism</subject><subject>Kidney - cytology</subject><subject>Methyltransferases - metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular and cellular biology</subject><subject>Protein Transport - drug effects</subject><subject>Quinones - chemistry</subject><subject>Quinones - metabolism</subject><subject>Quinones - pharmacology</subject><subject>Rats</subject><subject>S-Adenosylmethionine - metabolism</subject><subject>S-Adenosylmethionine - pharmacology</subject><subject>Structure-Activity Relationship</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0NtKxDAQBuAgiq6HR1ACgoeL6mSTtol3i3gCwQsVvFuy6VQj2bRmWmGfwxe2i6t4NTff_88wjO0LOBMgivNHMAVk2qiXE4BTgEKU2csaGwlVqEwqyNfZ6I9ssW2idwChQKlNtiXEGAql5Ih9PWaTCmNDizDH7s030UfkCT8xERL3wc_tR-9jE_GY-CeSd32w3eB4U_PuDflNE149t21rk-16uuATXoe-SUgOo0NOXV8t-OCX2GEIQz5xHztM1i2LaNn0f9Eu26htINxbzR32fH31dHmb3T_c3F1O7rN2LPMuM2Mp0dQKC9ClmUGuS22VLXKHxhiXy7xyEosScoSqlq4al_WsQiGMVhYrlDvs6Ke3Tc1Hj9RN556WF9qITU_TIam1lmqAByvYz-ZYTds0HJsW0983DuBwBSw5G-pko_P053QpJJTyG8QjhoM</recordid><startdate>20010122</startdate><enddate>20010122</enddate><creator>CASAUBON, Rebecca L</creator><creator>SNAPPER, Marc L</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010122</creationdate><title>S-Adenosylmethionine reverses ilimaquinone's vesiculation of the Golgi apparatus: A fluorescence study on the cellular interactions of ilimaquinone</title><author>CASAUBON, Rebecca L ; SNAPPER, Marc L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-9233e9f4e60879b05878a4a65ce999c535dc3e6705e0df3cd27fbde11984aede3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenosylhomocysteinase</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Cells, Cultured</topic><topic>Cytoplasmic Vesicles - drug effects</topic><topic>Cytoplasmic Vesicles - metabolism</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Golgi apparatus</topic><topic>Golgi Apparatus - drug effects</topic><topic>Hydrolases - metabolism</topic><topic>Kidney - cytology</topic><topic>Methyltransferases - metabolism</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular and cellular biology</topic><topic>Protein Transport - drug effects</topic><topic>Quinones - chemistry</topic><topic>Quinones - metabolism</topic><topic>Quinones - pharmacology</topic><topic>Rats</topic><topic>S-Adenosylmethionine - metabolism</topic><topic>S-Adenosylmethionine - pharmacology</topic><topic>Structure-Activity Relationship</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CASAUBON, Rebecca L</creatorcontrib><creatorcontrib>SNAPPER, Marc L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CASAUBON, Rebecca L</au><au>SNAPPER, Marc L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>S-Adenosylmethionine reverses ilimaquinone's vesiculation of the Golgi apparatus: A fluorescence study on the cellular interactions of ilimaquinone</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2001-01-22</date><risdate>2001</risdate><volume>11</volume><issue>2</issue><spage>133</spage><epage>136</epage><pages>133-136</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>The marine sponge metabolite ilimaquinone has a wide range of biological activities, including vesiculation of the Golgi apparatus and interference with intracellular protein trafficking. Some of these activities may arise from ilimaquinone's influence on the activated methyl cycle. To visualize the morphological effects of ilimaquinone on the Golgi apparatus, NRK (normal rat kidney) cells were labeled with fluorescent wheat germ agglutinin and treated with ilimaquinone in the presence and absence of the methylating agent S-adenosylmethionine (SAMe). While ilimaquinone alone fragments the Golgi apparatus, the organelle remains intact when SAMe is included in the incubation mixture. This observation supports ilimaquinone's interaction with methylation enzymes as the cause of Golgi vesiculation. The examination of a fluorescently labeled ilimaquinone analogue in NRK cells suggests that the cellular interactions of ilimaquinone are not localized to the Golgi apparatus.</abstract><cop>Oxford</cop><pub>Elsevier</pub><pmid>11206443</pmid><doi>10.1016/S0960-894X(00)00617-X</doi><tpages>4</tpages></addata></record> |
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| ispartof | Bioorganic & medicinal chemistry letters, 2001-01, Vol.11 (2), p.133-136 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosylhomocysteinase Animals Biological and medical sciences Cell structures and functions Cells, Cultured Cytoplasmic Vesicles - drug effects Cytoplasmic Vesicles - metabolism Fluorescent Dyes Fundamental and applied biological sciences. Psychology Golgi apparatus Golgi Apparatus - drug effects Hydrolases - metabolism Kidney - cytology Methyltransferases - metabolism Microscopy, Fluorescence Molecular and cellular biology Protein Transport - drug effects Quinones - chemistry Quinones - metabolism Quinones - pharmacology Rats S-Adenosylmethionine - metabolism S-Adenosylmethionine - pharmacology Structure-Activity Relationship |
| title | S-Adenosylmethionine reverses ilimaquinone's vesiculation of the Golgi apparatus: A fluorescence study on the cellular interactions of ilimaquinone |
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