Butyrate-Induced Differentiation of Caco-2 Cells Occurs Independently from p27
Butyrate, a short-chain fatty acid produced in the colon, reduces proliferation and increases differentiation of colon cancer cells. p27, an inhibitor of cyclin-dependent kinases and a negative regulator of the cell cycle, is thought to have a key function in the differentiation of various cell line...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2001-02, Vol.281 (2), p.295-299 |
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| creator | Wächtershäuser, Astrid Stein, Jürgen |
| description | Butyrate, a short-chain fatty acid produced in the colon, reduces proliferation and increases differentiation of colon cancer cells. p27, an inhibitor of cyclin-dependent kinases and a negative regulator of the cell cycle, is thought to have a key function in the differentiation of various cell lines. The objective of the present study was to elucidate the role of p27 in butyrate-induced differentiation of the human colorectal carcinoma cell line Caco-2. In this report we show that in spite of the increase in p27 protein expression after incubation with the HMG-CoA reductase inhibitor mevastatin, alkaline phosphatase activity decreases significantly in this cell line. In addition, mevastatin caused a significant increase in the cell cycle inhibitor p21. All effects could be reversed by addition of mevalonate to the medium. Taken together, we provide the first evidence that in Caco-2 cells p27 may have other functions apart from the regulation of cell differentiation. |
| doi_str_mv | 10.1006/bbrc.2001.4346 |
| format | Article |
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The objective of the present study was to elucidate the role of p27 in butyrate-induced differentiation of the human colorectal carcinoma cell line Caco-2. In this report we show that in spite of the increase in p27 protein expression after incubation with the HMG-CoA reductase inhibitor mevastatin, alkaline phosphatase activity decreases significantly in this cell line. In addition, mevastatin caused a significant increase in the cell cycle inhibitor p21. All effects could be reversed by addition of mevalonate to the medium. Taken together, we provide the first evidence that in Caco-2 cells p27 may have other functions apart from the regulation of cell differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2001.4346</identifier><identifier>PMID: 11181044</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Apoptosis - drug effects ; butyrate ; Butyrates - pharmacology ; Caco-2 Cells ; cell cycle ; Cell Cycle Proteins ; Cell Differentiation - drug effects ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclin-Dependent Kinase Inhibitor p27 ; Cyclins - drug effects ; Cyclins - metabolism ; differentiation ; Dose-Response Relationship, Drug ; HMG-CoA reductase ; Humans ; Hydroxymethylglutaryl CoA Reductases - drug effects ; Hydroxymethylglutaryl CoA Reductases - metabolism ; Lovastatin - analogs & derivatives ; Lovastatin - pharmacology ; mevastatin ; Microtubule-Associated Proteins - drug effects ; Microtubule-Associated Proteins - metabolism ; Microtubule-Associated Proteins - physiology ; p27 ; Tumor Suppressor Proteins ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2001-02, Vol.281 (2), p.295-299</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-beb703868d5e0c0ccb4866fd295e0f82f358098ae4244093895e4c630f565b433</citedby><cites>FETCH-LOGICAL-c340t-beb703868d5e0c0ccb4866fd295e0f82f358098ae4244093895e4c630f565b433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X01943463$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11181044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wächtershäuser, Astrid</creatorcontrib><creatorcontrib>Stein, Jürgen</creatorcontrib><title>Butyrate-Induced Differentiation of Caco-2 Cells Occurs Independently from p27</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Butyrate, a short-chain fatty acid produced in the colon, reduces proliferation and increases differentiation of colon cancer cells. p27, an inhibitor of cyclin-dependent kinases and a negative regulator of the cell cycle, is thought to have a key function in the differentiation of various cell lines. The objective of the present study was to elucidate the role of p27 in butyrate-induced differentiation of the human colorectal carcinoma cell line Caco-2. In this report we show that in spite of the increase in p27 protein expression after incubation with the HMG-CoA reductase inhibitor mevastatin, alkaline phosphatase activity decreases significantly in this cell line. In addition, mevastatin caused a significant increase in the cell cycle inhibitor p21. All effects could be reversed by addition of mevalonate to the medium. Taken together, we provide the first evidence that in Caco-2 cells p27 may have other functions apart from the regulation of cell differentiation.</description><subject>Apoptosis - drug effects</subject><subject>butyrate</subject><subject>Butyrates - pharmacology</subject><subject>Caco-2 Cells</subject><subject>cell cycle</subject><subject>Cell Cycle Proteins</subject><subject>Cell Differentiation - drug effects</subject><subject>Cyclin-Dependent Kinase Inhibitor p21</subject><subject>Cyclin-Dependent Kinase Inhibitor p27</subject><subject>Cyclins - drug effects</subject><subject>Cyclins - metabolism</subject><subject>differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>HMG-CoA reductase</subject><subject>Humans</subject><subject>Hydroxymethylglutaryl CoA Reductases - drug effects</subject><subject>Hydroxymethylglutaryl CoA Reductases - metabolism</subject><subject>Lovastatin - analogs & derivatives</subject><subject>Lovastatin - pharmacology</subject><subject>mevastatin</subject><subject>Microtubule-Associated Proteins - drug effects</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Microtubule-Associated Proteins - physiology</subject><subject>p27</subject><subject>Tumor Suppressor Proteins</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LxDAQhoMo7rp69Sg9eWudpGk3PWr9Wljci4K30KYTiLRNTVph_70pu-DJS4YMz7zMPIRcU0goQH5X104lDIAmPOX5CVlSKCBmFPgpWUIgYlbQzwW58P4rUJTnxTlZUEpFQPiSvD1M495VI8abvpkUNtGj0Rod9qOpRmP7yOqorJSNWVRi2_pop9TkfBRwHDA8_djuI-1sFw1sfUnOdNV6vDrWFfl4fnovX-Pt7mVT3m9jlXIY4xrrNaQiF02GoECpmos81w0rwl8LptNMQCEq5IxzKFIR-lzlKegsz2qepitye8gdnP2e0I-yM16F_aoe7eTlGjIhBKMBTA6gctZ7h1oOznSV20sKcjYoZ4NyNihng2Hg5pg81R02f_hRWQDEAcBw349BJ70y2Ad1xqEaZWPNf9m_vUx96A</recordid><startdate>20010223</startdate><enddate>20010223</enddate><creator>Wächtershäuser, Astrid</creator><creator>Stein, Jürgen</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010223</creationdate><title>Butyrate-Induced Differentiation of Caco-2 Cells Occurs Independently from p27</title><author>Wächtershäuser, Astrid ; Stein, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-beb703868d5e0c0ccb4866fd295e0f82f358098ae4244093895e4c630f565b433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Apoptosis - drug effects</topic><topic>butyrate</topic><topic>Butyrates - pharmacology</topic><topic>Caco-2 Cells</topic><topic>cell cycle</topic><topic>Cell Cycle Proteins</topic><topic>Cell Differentiation - drug effects</topic><topic>Cyclin-Dependent Kinase Inhibitor p21</topic><topic>Cyclin-Dependent Kinase Inhibitor p27</topic><topic>Cyclins - drug effects</topic><topic>Cyclins - metabolism</topic><topic>differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>HMG-CoA reductase</topic><topic>Humans</topic><topic>Hydroxymethylglutaryl CoA Reductases - drug effects</topic><topic>Hydroxymethylglutaryl CoA Reductases - metabolism</topic><topic>Lovastatin - analogs & derivatives</topic><topic>Lovastatin - pharmacology</topic><topic>mevastatin</topic><topic>Microtubule-Associated Proteins - drug effects</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Microtubule-Associated Proteins - physiology</topic><topic>p27</topic><topic>Tumor Suppressor Proteins</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wächtershäuser, Astrid</creatorcontrib><creatorcontrib>Stein, Jürgen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wächtershäuser, Astrid</au><au>Stein, Jürgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Butyrate-Induced Differentiation of Caco-2 Cells Occurs Independently from p27</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2001-02-23</date><risdate>2001</risdate><volume>281</volume><issue>2</issue><spage>295</spage><epage>299</epage><pages>295-299</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Butyrate, a short-chain fatty acid produced in the colon, reduces proliferation and increases differentiation of colon cancer cells. p27, an inhibitor of cyclin-dependent kinases and a negative regulator of the cell cycle, is thought to have a key function in the differentiation of various cell lines. The objective of the present study was to elucidate the role of p27 in butyrate-induced differentiation of the human colorectal carcinoma cell line Caco-2. In this report we show that in spite of the increase in p27 protein expression after incubation with the HMG-CoA reductase inhibitor mevastatin, alkaline phosphatase activity decreases significantly in this cell line. In addition, mevastatin caused a significant increase in the cell cycle inhibitor p21. All effects could be reversed by addition of mevalonate to the medium. Taken together, we provide the first evidence that in Caco-2 cells p27 may have other functions apart from the regulation of cell differentiation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11181044</pmid><doi>10.1006/bbrc.2001.4346</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2001-02, Vol.281 (2), p.295-299 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Apoptosis - drug effects butyrate Butyrates - pharmacology Caco-2 Cells cell cycle Cell Cycle Proteins Cell Differentiation - drug effects Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclins - drug effects Cyclins - metabolism differentiation Dose-Response Relationship, Drug HMG-CoA reductase Humans Hydroxymethylglutaryl CoA Reductases - drug effects Hydroxymethylglutaryl CoA Reductases - metabolism Lovastatin - analogs & derivatives Lovastatin - pharmacology mevastatin Microtubule-Associated Proteins - drug effects Microtubule-Associated Proteins - metabolism Microtubule-Associated Proteins - physiology p27 Tumor Suppressor Proteins Up-Regulation |
| title | Butyrate-Induced Differentiation of Caco-2 Cells Occurs Independently from p27 |
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