Human antibody recognition of Anisakidae and Trichinella spp. in Greenland
High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antige...
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| Veröffentlicht in: | Clinical microbiology and infection 2007-07, Vol.13 (7), p.702-708 |
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| creator | Møller, L.N. Grove Krause, T. Koch, A. Melbye, M. Kapel, C.M.O. Petersen, E. |
| description | High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antigens from Anisakidae larvae. Western blots with Anisakidae and Trichinella antigens discriminated between Anisakidae and Trichinella infections, enabling cross-reactivity between the two parasite infections to be eliminated. Serum samples from 1012 children in Greenland were analysed for specific antibodies to Anisakidae and Trichinella. Eleven children were IgG-positive for Trichinella and nine were IgG-positive for Anisakidae, indicating a relatively low prevalence of both infections among children in Greenland. Faecal samples from 320 children were also examined for other intestinal parasites. Enterobius vermicularis was found in one sample and Blastocystis hominis in 32 samples, but no other intestinal parasites were identified. In total, 304 children had elevated total IgE levels. There was a significant association between Trichinella seropositivity and high levels of total IgE, but not between Anisakidae seropositivity and total IgE. The data indicate that parasitic infections alone do not explain the high level of total IgE observed among children in Greenland. |
| doi_str_mv | 10.1111/j.1469-0691.2007.01730.x |
| format | Article |
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To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antigens from Anisakidae larvae. Western blots with Anisakidae and Trichinella antigens discriminated between Anisakidae and Trichinella infections, enabling cross-reactivity between the two parasite infections to be eliminated. Serum samples from 1012 children in Greenland were analysed for specific antibodies to Anisakidae and Trichinella. Eleven children were IgG-positive for Trichinella and nine were IgG-positive for Anisakidae, indicating a relatively low prevalence of both infections among children in Greenland. Faecal samples from 320 children were also examined for other intestinal parasites. Enterobius vermicularis was found in one sample and Blastocystis hominis in 32 samples, but no other intestinal parasites were identified. In total, 304 children had elevated total IgE levels. There was a significant association between Trichinella seropositivity and high levels of total IgE, but not between Anisakidae seropositivity and total IgE. The data indicate that parasitic infections alone do not explain the high level of total IgE observed among children in Greenland.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1111/j.1469-0691.2007.01730.x</identifier><identifier>PMID: 17484764</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Adolescent ; Animals ; Anisakidae ; Antibodies, Helminth - blood ; Antibodies, Helminth - immunology ; Antibody Specificity ; Antigens, Helminth - immunology ; Ascaridida Infections - epidemiology ; Ascaridida Infections - immunology ; Ascaridida Infections - parasitology ; Ascaridoidea - growth & development ; Ascaridoidea - immunology ; Biological and medical sciences ; Blastocystis hominis ; Child ; Diseases caused by nematodes ; ELISA ; Enterobius vermicularis ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; General aspects ; Greenland ; Greenland - epidemiology ; Helminthic diseases ; Humans ; IgE ; immunoblot ; Immunoglobulin E - blood ; Immunoglobulin G - blood ; Infectious diseases ; Larva - immunology ; Male ; Medical sciences ; Parasitic diseases ; Trichinella ; Trichinella - immunology ; Trichinella spp ; Trichinellosis - epidemiology ; Trichinellosis - immunology ; Trichinellosis - parasitology ; Trichinosis</subject><ispartof>Clinical microbiology and infection, 2007-07, Vol.13 (7), p.702-708</ispartof><rights>2007 European Society of Clinical Infectious Diseases</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5370-d0277c68182c0d613ba1fc6cfe91b182c6b4780870a399982ccc906603c8cb633</citedby><cites>FETCH-LOGICAL-c5370-d0277c68182c0d613ba1fc6cfe91b182c6b4780870a399982ccc906603c8cb633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1469-0691.2007.01730.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1469-0691.2007.01730.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18818247$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17484764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Møller, L.N.</creatorcontrib><creatorcontrib>Grove Krause, T.</creatorcontrib><creatorcontrib>Koch, A.</creatorcontrib><creatorcontrib>Melbye, M.</creatorcontrib><creatorcontrib>Kapel, C.M.O.</creatorcontrib><creatorcontrib>Petersen, E.</creatorcontrib><title>Human antibody recognition of Anisakidae and Trichinella spp. in Greenland</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antigens from Anisakidae larvae. Western blots with Anisakidae and Trichinella antigens discriminated between Anisakidae and Trichinella infections, enabling cross-reactivity between the two parasite infections to be eliminated. Serum samples from 1012 children in Greenland were analysed for specific antibodies to Anisakidae and Trichinella. Eleven children were IgG-positive for Trichinella and nine were IgG-positive for Anisakidae, indicating a relatively low prevalence of both infections among children in Greenland. Faecal samples from 320 children were also examined for other intestinal parasites. Enterobius vermicularis was found in one sample and Blastocystis hominis in 32 samples, but no other intestinal parasites were identified. In total, 304 children had elevated total IgE levels. There was a significant association between Trichinella seropositivity and high levels of total IgE, but not between Anisakidae seropositivity and total IgE. The data indicate that parasitic infections alone do not explain the high level of total IgE observed among children in Greenland.</description><subject>Adolescent</subject><subject>Animals</subject><subject>Anisakidae</subject><subject>Antibodies, Helminth - blood</subject><subject>Antibodies, Helminth - immunology</subject><subject>Antibody Specificity</subject><subject>Antigens, Helminth - immunology</subject><subject>Ascaridida Infections - epidemiology</subject><subject>Ascaridida Infections - immunology</subject><subject>Ascaridida Infections - parasitology</subject><subject>Ascaridoidea - growth & development</subject><subject>Ascaridoidea - immunology</subject><subject>Biological and medical sciences</subject><subject>Blastocystis hominis</subject><subject>Child</subject><subject>Diseases caused by nematodes</subject><subject>ELISA</subject><subject>Enterobius vermicularis</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>General aspects</subject><subject>Greenland</subject><subject>Greenland - epidemiology</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>IgE</subject><subject>immunoblot</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious diseases</subject><subject>Larva - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Trichinella</subject><subject>Trichinella - immunology</subject><subject>Trichinella spp</subject><subject>Trichinellosis - epidemiology</subject><subject>Trichinellosis - immunology</subject><subject>Trichinellosis - parasitology</subject><subject>Trichinosis</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vGyEQQFHVqEnc_IWKS3vbzbAQYA89pFbzJUe5pFJuiJ1lE9w164Kd2v8-bG0lx5QLaObNMDwIoQxKltfpvGRC1gXImpUVgCqBKQ7l5gM5ek18zGdW60IJ_nBIjlOaA0DFufhEDpkSWigpjsjN1XphA7Vh5Zuh3dLocHgMfuWHQIeOngef7G_fWpeRlt5Hj08-uL63NC2XJfWBXkbnQp-zn8lBZ_vkTvb7hPy6-Hk_vSpmd5fX0_NZgWdcQdFCpRRKzXSF0ErGG8s6lNi5mjVjUDZCadAKLK_rOgcQa5ASOGpsJOcT8m3XdxmHP2uXVmbhE44zBTesk1FwprUS1btgBUJwyI0nRO9AjENK0XVmGf3Cxq1hYEbhZm5Gr2b0akbh5p9ws8mlX_Z3rJuFa98K94Yz8HUP2IS276IN6NMbp0cRQmXu-47763u3_e8BzHR2O55y_Y9dvcvqn72LJqF3AV3r85-uTDv491_zAqXDr2Q</recordid><startdate>200707</startdate><enddate>200707</enddate><creator>Møller, L.N.</creator><creator>Grove Krause, T.</creator><creator>Koch, A.</creator><creator>Melbye, M.</creator><creator>Kapel, C.M.O.</creator><creator>Petersen, E.</creator><general>Elsevier Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200707</creationdate><title>Human antibody recognition of Anisakidae and Trichinella spp. in Greenland</title><author>Møller, L.N. ; Grove Krause, T. ; Koch, A. ; Melbye, M. ; Kapel, C.M.O. ; Petersen, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5370-d0277c68182c0d613ba1fc6cfe91b182c6b4780870a399982ccc906603c8cb633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Animals</topic><topic>Anisakidae</topic><topic>Antibodies, Helminth - blood</topic><topic>Antibodies, Helminth - immunology</topic><topic>Antibody Specificity</topic><topic>Antigens, Helminth - immunology</topic><topic>Ascaridida Infections - epidemiology</topic><topic>Ascaridida Infections - immunology</topic><topic>Ascaridida Infections - parasitology</topic><topic>Ascaridoidea - growth & development</topic><topic>Ascaridoidea - immunology</topic><topic>Biological and medical sciences</topic><topic>Blastocystis hominis</topic><topic>Child</topic><topic>Diseases caused by nematodes</topic><topic>ELISA</topic><topic>Enterobius vermicularis</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>General aspects</topic><topic>Greenland</topic><topic>Greenland - epidemiology</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>IgE</topic><topic>immunoblot</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin G - blood</topic><topic>Infectious diseases</topic><topic>Larva - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Trichinella</topic><topic>Trichinella - immunology</topic><topic>Trichinella spp</topic><topic>Trichinellosis - epidemiology</topic><topic>Trichinellosis - immunology</topic><topic>Trichinellosis - parasitology</topic><topic>Trichinosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Møller, L.N.</creatorcontrib><creatorcontrib>Grove Krause, T.</creatorcontrib><creatorcontrib>Koch, A.</creatorcontrib><creatorcontrib>Melbye, M.</creatorcontrib><creatorcontrib>Kapel, C.M.O.</creatorcontrib><creatorcontrib>Petersen, E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Møller, L.N.</au><au>Grove Krause, T.</au><au>Koch, A.</au><au>Melbye, M.</au><au>Kapel, C.M.O.</au><au>Petersen, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human antibody recognition of Anisakidae and Trichinella spp. in Greenland</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2007-07</date><risdate>2007</risdate><volume>13</volume><issue>7</issue><spage>702</spage><epage>708</epage><pages>702-708</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antigens from Anisakidae larvae. Western blots with Anisakidae and Trichinella antigens discriminated between Anisakidae and Trichinella infections, enabling cross-reactivity between the two parasite infections to be eliminated. Serum samples from 1012 children in Greenland were analysed for specific antibodies to Anisakidae and Trichinella. Eleven children were IgG-positive for Trichinella and nine were IgG-positive for Anisakidae, indicating a relatively low prevalence of both infections among children in Greenland. Faecal samples from 320 children were also examined for other intestinal parasites. Enterobius vermicularis was found in one sample and Blastocystis hominis in 32 samples, but no other intestinal parasites were identified. In total, 304 children had elevated total IgE levels. There was a significant association between Trichinella seropositivity and high levels of total IgE, but not between Anisakidae seropositivity and total IgE. The data indicate that parasitic infections alone do not explain the high level of total IgE observed among children in Greenland.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>17484764</pmid><doi>10.1111/j.1469-0691.2007.01730.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical microbiology and infection, 2007-07, Vol.13 (7), p.702-708 |
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| subjects | Adolescent Animals Anisakidae Antibodies, Helminth - blood Antibodies, Helminth - immunology Antibody Specificity Antigens, Helminth - immunology Ascaridida Infections - epidemiology Ascaridida Infections - immunology Ascaridida Infections - parasitology Ascaridoidea - growth & development Ascaridoidea - immunology Biological and medical sciences Blastocystis hominis Child Diseases caused by nematodes ELISA Enterobius vermicularis Enzyme-Linked Immunosorbent Assay - methods Female General aspects Greenland Greenland - epidemiology Helminthic diseases Humans IgE immunoblot Immunoglobulin E - blood Immunoglobulin G - blood Infectious diseases Larva - immunology Male Medical sciences Parasitic diseases Trichinella Trichinella - immunology Trichinella spp Trichinellosis - epidemiology Trichinellosis - immunology Trichinellosis - parasitology Trichinosis |
| title | Human antibody recognition of Anisakidae and Trichinella spp. in Greenland |
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