Use of western immunoblot for evaluation of myocardial dystrophin, alpha-sarcoglycan, and beta-dystroglycan in dogs with idiopathic dilated cardiomyopathy

To evaluate the potential importance of dystrophin, alpha-sarcoglycan (adhalin), and beta-dystroglycan, by use of western blot analysis, in several breeds of dogs with dilated cardiomyopathy. Myocardial samples obtained from 12 dogs were evaluated, including tissues from 7 dogs affected with dilated...

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Veröffentlicht in:American journal of veterinary research 2001-01, Vol.62 (1), p.67-71
Hauptverfasser: Spier, A W, Meurs, K M, Coovert, D D, Lehmkuhl, L B, O'Grady, M R, Freeman, L M, Burghes, A H, Towbin, J A
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container_end_page 71
container_issue 1
container_start_page 67
container_title American journal of veterinary research
container_volume 62
creator Spier, A W
Meurs, K M
Coovert, D D
Lehmkuhl, L B
O'Grady, M R
Freeman, L M
Burghes, A H
Towbin, J A
description To evaluate the potential importance of dystrophin, alpha-sarcoglycan (adhalin), and beta-dystroglycan, by use of western blot analysis, in several breeds of dogs with dilated cardiomyopathy. Myocardial samples obtained from 12 dogs were evaluated, including tissues from 7 dogs affected with dilated cardiomyopathy, 4 control dogs with no identifiable heart disease (positive control), and 1 dog affected with Duchenne muscular dystrophy (negative control for dystrophin). Of the affected dogs, 4 breeds were represented (Doberman Pinscher, Dalmatian, Bullmastiff, and Irish Wolfhound). Western blot analysis was used for evaluation of myocardial samples obtained from dogs with and without dilated cardiomyopathy for the presence of dystrophin and 2 of its associated glycoproteins, alpha-sarcoglycan and beta-dystroglycan. Detectable differences were not identified between dogs with and without myocardial disease in any of the proteins evaluated. Abnormalities in dystrophin, alpha-sarcoglycan, and beta-dystroglycan proteins were not associated with the development of dilated cardiomyopathy in the dogs evaluated in this study. In humans, the development of molecular biological techniques has allowed for the identification of specific causes of dilated cardiomyopathy that were once considered to be idiopathic. The use of similar techniques in veterinary medicine may aid in the identification of the cause of idiopathic dilated cardiomyopathy in dogs, and may offer new avenues for therapeutic intervention.
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Myocardial samples obtained from 12 dogs were evaluated, including tissues from 7 dogs affected with dilated cardiomyopathy, 4 control dogs with no identifiable heart disease (positive control), and 1 dog affected with Duchenne muscular dystrophy (negative control for dystrophin). Of the affected dogs, 4 breeds were represented (Doberman Pinscher, Dalmatian, Bullmastiff, and Irish Wolfhound). Western blot analysis was used for evaluation of myocardial samples obtained from dogs with and without dilated cardiomyopathy for the presence of dystrophin and 2 of its associated glycoproteins, alpha-sarcoglycan and beta-dystroglycan. Detectable differences were not identified between dogs with and without myocardial disease in any of the proteins evaluated. Abnormalities in dystrophin, alpha-sarcoglycan, and beta-dystroglycan proteins were not associated with the development of dilated cardiomyopathy in the dogs evaluated in this study. In humans, the development of molecular biological techniques has allowed for the identification of specific causes of dilated cardiomyopathy that were once considered to be idiopathic. 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In humans, the development of molecular biological techniques has allowed for the identification of specific causes of dilated cardiomyopathy that were once considered to be idiopathic. 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subjects Animals
Blotting, Western
Cardiomyopathy, Dilated - metabolism
Cardiomyopathy, Dilated - veterinary
Cytoskeletal Proteins - analysis
Dog Diseases - metabolism
Dogs
Dystroglycans
Dystrophin - analysis
Membrane Glycoproteins - analysis
Muscular Dystrophy, Animal - metabolism
Muscular Dystrophy, Duchenne - metabolism
Myocardium - chemistry
Receptors, Laminin - analysis
Reference Values
Sarcoglycans
title Use of western immunoblot for evaluation of myocardial dystrophin, alpha-sarcoglycan, and beta-dystroglycan in dogs with idiopathic dilated cardiomyopathy
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