A general purpose method for extracting RNA from Dictyostelium cells

Here we present a protocol for the extraction of RNA from Dictyostelium discoideum . Dictyostelium is a social amoeba that undergoes a basic developmental program, and therefore analysis of RNA levels over a time course is a commonly used technique. This procedure is similar to other guanidine thioc...

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Veröffentlicht in:	Nature protocols 2007-06, Vol.2 (6), p.1329-1332
Hauptverfasser:	Chisholm, Rex L, Pilcher, Karen E, Gaudet, Pascale, Fey, Petra, Kowal, Anthony S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amoeba Analytical Chemistry Animals Biological Techniques Biomedical and Life Sciences Chloroform Chloroform - chemistry Computational Biology/Bioinformatics Dictyostelium Dictyostelium - chemistry Dictyostelium - genetics Dictyostelium discoideum DNA microarrays Genetic aspects Life Sciences Microarrays Organic Chemistry Phenol - chemistry Phenols Physiological aspects protocol RNA RNA - chemistry RNA - isolation & purification Time Factors
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